This is a Validated Antibody Database (VAD) review about human USP4, based on 4 published articles (read how Labome selects the articles), using USP4 antibody in all methods. It is aimed to help Labome visitors find the most suited USP4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
USP4 synonym: UNP; Unph; ubiquitin carboxyl-terminal hydrolase 4; deubiquitinating enzyme 4; ubiquitin carboxyl-terminal esterase 4; ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin specific protease 4 (proto-oncogene); ubiquitin thioesterase 4; ubiquitin thiolesterase 4; ubiquitin-specific processing protease 4; ubiquitous nuclear protein homolog

Bethyl
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4b
In order to develop a method to assess deubiquitylating enzyme activity in mouse tissues and use it to evaluate deubiquitylating enzyme inhibitors, Bethyl USP4 antibody (Bethyl, A300- 830A) was used in western blot on mouse samples at 1:1000 (fig 4b). Methods Mol Biol (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 3e
  • western blot; human; fig 3c
Bethyl USP4 antibody (Bethyl Laboratories, A300-830A) was used in immunoprecipitation on human samples (fig 3e) and in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig s4
Bethyl USP4 antibody (Bethyl, A300-830A) was used in immunoprecipitation on human samples (fig s4). Sci Rep (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-3)
  • immunohistochemistry - paraffin section; human; fig 6
  • western blot; human; fig 1
Santa Cruz Biotechnology USP4 antibody (Santa Cruz Biotechnology, SC-376000) was used in immunohistochemistry - paraffin section on human samples (fig 6) and in western blot on human samples (fig 1). Oncogene (2016) ncbi
Articles Reviewed
  1. McLellan L, Forder C, Cranston A, Harrigan J, Jacq X. Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues. Methods Mol Biol. 2016;1449:411-9 pubmed publisher
  2. Hwang S, Lee H, Kim H, Lee H, Shin C, Yun S, et al. Ubiquitin-specific protease 4 controls metastatic potential through β-catenin stabilization in brain metastatic lung adenocarcinoma. Sci Rep. 2016;6:21596 pubmed publisher
  3. Li Z, Hao Q, Luo J, Xiong J, Zhang S, Wang T, et al. USP4 inhibits p53 and NF-κB through deubiquitinating and stabilizing HDAC2. Oncogene. 2016;35:2902-12 pubmed publisher
  4. Grou C, Pinto M, Mendes A, Domingues P, Azevedo J. The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors. Sci Rep. 2015;5:12836 pubmed publisher