This is a Validated Antibody Database (VAD) review about human USP14, based on 7 published articles (read how Labome selects the articles), using USP14 antibody in all methods. It is aimed to help Labome visitors find the most suited USP14 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
USP14 synonym: TGT; ubiquitin carboxyl-terminal hydrolase 14; deubiquitinating enzyme 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA-guanine transglycosylase); ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14

Bethyl
rabbit polyclonal
  • western blot; human; fig 2
Bethyl USP14 antibody (Bethyl Laboratories, A300-919A) was used in western blot on human samples (fig 2). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Bethyl USP14 antibody (Bethyl laboratories, A300-920A) was used in western blot on human samples (fig 5). Chem Biol Drug Des (2015) ncbi
Abcam
rabbit monoclonal (EPR15943)
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam USP14 antibody (Abcam, ab192618) was used in western blot on human samples at 1:1000 (fig 4d). Biochem Pharmacol (2017) ncbi
GeneTex
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 5a
In order to elucidate the role of HAP40 in Huntington's disease, GeneTex USP14 antibody (GeneTex, GTX115186) was used in western blot on mouse samples at 1:1000 (fig 5a). Mol Neurobiol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (6E6)
  • western blot; mouse
Santa Cruz Biotechnology USP14 antibody (Santa Cruz Biotechnology, sc-100630) was used in western blot on mouse samples . J Neuroinflammation (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D8Q6S)
  • western blot; mouse; 1:1000; loading ...; fig 4k
Cell Signaling Technology USP14 antibody (Cell Signaling, 11931) was used in western blot on mouse samples at 1:1000 (fig 4k). Dis Model Mech (2017) ncbi
rabbit monoclonal (D8Q6S)
  • western blot; mouse; 1:2000; loading ...; fig 4b
In order to develop a method to assess deubiquitylating enzyme activity in mouse tissues and use it to evaluate deubiquitylating enzyme inhibitors, Cell Signaling Technology USP14 antibody (Cell Signaling, 11931S) was used in western blot on mouse samples at 1:2000 (fig 4b). Methods Mol Biol (2016) ncbi
Articles Reviewed
  1. Lang F, Aravamudhan S, Nolte H, Türk C, Hölper S, Muller S, et al. Dynamic changes in the mouse skeletal muscle proteome during denervation-induced atrophy. Dis Model Mech. 2017;10:881-896 pubmed publisher
  2. Yue X, Zuo Y, Ke H, Luo J, Lou L, Qin W, et al. Identification of 4-arylidene curcumin analogues as novel proteasome inhibitors for potential anticancer agents targeting 19S regulatory particle associated deubiquitinase. Biochem Pharmacol. 2017;137:29-50 pubmed publisher
  3. Huang Z, Her L. The Ubiquitin Receptor ADRM1 Modulates HAP40-Induced Proteasome Activity. Mol Neurobiol. 2017;54:7382-7400 pubmed publisher
  4. McLellan L, Forder C, Cranston A, Harrigan J, Jacq X. Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues. Methods Mol Biol. 2016;1449:411-9 pubmed publisher
  5. Wang X, Mazurkiewicz M, Hillert E, Olofsson M, Pierrou S, Hillertz P, et al. The proteasome deubiquitinase inhibitor VLX1570 shows selectivity for ubiquitin-specific protease-14 and induces apoptosis of multiple myeloma cells. Sci Rep. 2016;6:26979 pubmed publisher
  6. Wang X, D Arcy P, Caulfield T, Paulus A, Chitta K, Mohanty C, et al. Synthesis and evaluation of derivatives of the proteasome deubiquitinase inhibitor b-AP15. Chem Biol Drug Des. 2015;86:1036-48 pubmed publisher
  7. Qu Z, Meng F, Zhou H, Li J, Wang Q, Wei F, et al. NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells. J Neuroinflammation. 2014;11:17 pubmed publisher