This is a Validated Antibody Database (VAD) review about human UPF2, based on 6 published articles (read how Labome selects the articles), using UPF2 antibody in all methods. It is aimed to help Labome visitors find the most suited UPF2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UPF2 synonym: HUPF2; RENT2; smg-3; regulator of nonsense transcripts 2; FRS2/UPF2/LEMD3 fusion; LEMD3/UPF2 fusion; UPF2 regulator of nonsense transcripts homolog; nonsense mRNA reducing factor 2; smg-3 homolog, nonsense mediated mRNA decay factor; up-frameshift suppressor 2 homolog; yeast Upf2p homolog

Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl UPF2 antibody (Bethyl, A303-929A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • proximity ligation assay; human; 1:1000; loading ...; fig 8c
  • western blot; human; 1:1000; loading ...; fig 2c
Bethyl UPF2 antibody (Bethyl Laboratories, A303-929A) was used in proximity ligation assay on human samples at 1:1000 (fig 8c) and in western blot on human samples at 1:1000 (fig 2c). PLoS ONE (2016) ncbi
GeneTex
rabbit polyclonal
  • western blot; mouse; fig 1c
GeneTex UPF2 antibody (GeneTex, GTX107694) was used in western blot on mouse samples (fig 1c). Sci Rep (2017) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • flow cytometry; human; fig 6
In order to characterize enhancement of transcriptome variability and metastatic fitness by highly variable cancer subpopulations, LifeSpan Biosciences UPF2 antibody (LSBio, LS-C160443) was used in flow cytometry on human samples (fig 6). Nat Commun (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D3B10)
  • immunoprecipitation; mouse; loading ...; fig 5a
Cell Signaling Technology UPF2 antibody (Cell Signaling Technology, D3B10) was used in immunoprecipitation on mouse samples (fig 5a). Proc Natl Acad Sci U S A (2018) ncbi
rabbit monoclonal (D3B10)
  • western blot; mouse; fig s1
Cell Signaling Technology UPF2 antibody (Cell Signaling, D3B10) was used in western blot on mouse samples (fig s1). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Higa M, Oka M, Fujihara Y, Masuda K, Yoneda Y, Kishimoto T. Regulation of inflammatory responses by dynamic subcellular localization of RNA-binding protein Arid5a. Proc Natl Acad Sci U S A. 2018;115:E1214-E1220 pubmed publisher
  2. Mooney C, Jimenez Mateos E, Engel T, Mooney C, Diviney M, Venø M, et al. RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 2017;7:41517 pubmed publisher
  3. Nguyen A, Yoshida M, Goodarzi H, Tavazoie S. Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitness. Nat Commun. 2016;7:11246 pubmed publisher
  4. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  5. Schweingruber C, Soffientini P, Ruepp M, Bachi A, Mühlemann O. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID). PLoS ONE. 2016;11:e0150239 pubmed publisher
  6. Sanchez G, Bondy Chorney E, Laframboise J, Paris G, Didillon A, Jasmin B, et al. A novel role for CARM1 in promoting nonsense-mediated mRNA decay: potential implications for spinal muscular atrophy. Nucleic Acids Res. 2016;44:2661-76 pubmed publisher