This is a Validated Antibody Database (VAD) review about human UPF1, based on 14 published articles (read how Labome selects the articles), using UPF1 antibody in all methods. It is aimed to help Labome visitors find the most suited UPF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UPF1 synonym: HUPF1; NORF1; RENT1; pNORF1; smg-2

Abcam
domestic rabbit monoclonal (EPR4681)
  • western blot; human; 1:1000; loading ...; fig 2e
Abcam UPF1 antibody (Abcam, ab109363) was used in western blot on human samples at 1:1000 (fig 2e). elife (2021) ncbi
domestic rabbit monoclonal (EPR4681)
  • other; human; 1:5000; loading ...; fig 4e
Abcam UPF1 antibody (Abcam, ab109363) was used in other on human samples at 1:5000 (fig 4e). elife (2020) ncbi
domestic rabbit monoclonal (EPR4681)
  • western blot; human; 1:1000; loading ...; fig e6g
Abcam UPF1 antibody (Abcam, ab109363) was used in western blot on human samples at 1:1000 (fig e6g). Nature (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 7b
  • western blot; human; loading ...; fig 7a
Abcam UPF1 antibody (Abcam, ab86057) was used in immunocytochemistry on human samples (fig 7b) and in western blot on human samples (fig 7a). Mol Cell (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • western blot; mouse; fig 4
In order to propose that a special mechanism of splicing occurs in the testis and brain whereby H2A.B.3 recruits RNA processing factors from splicing speckles, Abcam UPF1 antibody (Abcam, ab109363) was used in western blot on mouse samples (fig 4). PLoS Genet (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
  • western blot; mouse; fig 1a
Abcam UPF1 antibody (Abcam, ab109363) was used in immunohistochemistry - paraffin section on mouse samples (fig 2a) and in western blot on mouse samples (fig 1a). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EPR4681)
  • western blot; human; loading ...; fig s8d
Abcam UPF1 antibody (Abcam, ab109363) was used in western blot on human samples (fig s8d). BMC Genomics (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-3)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1d
Santa Cruz Biotechnology UPF1 antibody (Santa Cruz Biotechnology, sc-166092) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1d). Int J Mol Med (2020) ncbi
mouse monoclonal (G-3)
  • immunoprecipitation; mouse; loading ...; fig 5a
  • immunocytochemistry; mouse; loading ...; fig 5d
Santa Cruz Biotechnology UPF1 antibody (Santa Cruz Biotechnology Inc, sc-166092) was used in immunoprecipitation on mouse samples (fig 5a) and in immunocytochemistry on mouse samples (fig 5d). Proc Natl Acad Sci U S A (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D15G6)
  • western blot; mouse; 1:1000; loading ...; fig e4a
  • western blot; human; 1:1000; loading ...; fig e7c
Cell Signaling Technology UPF1 antibody (Cell Signaling, 12040) was used in western blot on mouse samples at 1:1000 (fig e4a) and in western blot on human samples at 1:1000 (fig e7c). Nat Med (2022) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1s1c
Cell Signaling Technology UPF1 antibody (CST, 9435) was used in western blot on mouse samples at 1:1000 (fig 1s1c). elife (2021) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human; loading ...; fig 4c
Cell Signaling Technology UPF1 antibody (cst, 12040) was used in western blot on human samples (fig 4c). PLoS ONE (2018) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human; loading ...; fig 4c
Cell Signaling Technology UPF1 antibody (Cell signaling, D15G6) was used in western blot on human samples (fig 4c). J Virol (2017) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human; fig 4a
In order to identify residues of Y14 that are needed for binding to the mRNA cap, Cell Signaling Technology UPF1 antibody (Cell Signaling Technology, 12040) was used in western blot on human samples (fig 4a). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D15G6)
  • western blot; human
Cell Signaling Technology UPF1 antibody (Cell Signaling Technology, 12040S) was used in western blot on human samples . Mol Cancer (2015) ncbi
Articles Reviewed
  1. Korobeynikov V, Lyashchenko A, Blanco Redondo B, Jafar Nejad P, Shneider N. Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis. Nat Med. 2022;28:104-116 pubmed publisher
  2. Polaski J, Udy D, Escobar Hoyos L, Askan G, Leach S, Ventura A, et al. The origins and consequences of UPF1 variants in pancreatic adenosquamous carcinoma. elife. 2021;10: pubmed publisher
  3. Li M, Xie Z, Li J, Lin J, Zheng G, Liu W, et al. GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation. elife. 2020;9: pubmed publisher
  4. Yaojia Cheng Y, Lu Q, Shi N, Zhou Q, Rong J, Li L, et al. Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG. Int J Mol Med. 2020;45:1163-1175 pubmed publisher
  5. Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
  6. Cheruiyot A, Li S, Nickless A, Roth R, Fitzpatrick J, You Z. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS ONE. 2018;13:e0204978 pubmed publisher
  7. Higa M, Oka M, Fujihara Y, Masuda K, Yoneda Y, Kishimoto T. Regulation of inflammatory responses by dynamic subcellular localization of RNA-binding protein Arid5a. Proc Natl Acad Sci U S A. 2018;115:E1214-E1220 pubmed publisher
  8. Feng Q, Jagannathan S, Bradley R. The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 Ubiquitin Ligase Activity. Mol Cell. 2017;67:239-251.e6 pubmed publisher
  9. Soboleva T, Parker B, Nekrasov M, Hart Smith G, Tay Y, Tng W, et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet. 2017;13:e1006633 pubmed publisher
  10. Mooney C, Jimenez Mateos E, Engel T, Mooney C, Diviney M, Venø M, et al. RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 2017;7:41517 pubmed publisher
  11. Balinsky C, Schmeisser H, Wells A, Ganesan S, Jin T, Singh K, et al. IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10. J Virol. 2017;91: pubmed publisher
  12. Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
  13. He X, Zhang P. Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair. Mol Cancer. 2015;14:158 pubmed publisher
  14. Maekawa S, Imamachi N, Irie T, Tani H, Matsumoto K, Mizutani R, et al. Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance. BMC Genomics. 2015;16:154 pubmed publisher