This is a Validated Antibody Database (VAD) review about human UCP1, based on 26 published articles (read how Labome selects the articles), using UCP1 antibody in all methods. It is aimed to help Labome visitors find the most suited UCP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UCP1 synonym: SLC25A7; UCP

Knockout validation
R&D Systems
mouse monoclonal (536435)
  • immunocytochemistry knockout validation; mouse; 1:300; loading ...; fig 6a
  • flow cytometry; mouse; loading ...; fig 6b
R&D Systems UCP1 antibody (R&D Systems, MAB6158) was used in immunocytochemistry knockout validation on mouse samples at 1:300 (fig 6a) and in flow cytometry on mouse samples (fig 6b). Cell Rep (2018) ncbi
Abcam
domestic rabbit monoclonal
  • immunohistochemistry; mouse; fig 1e
  • western blot; mouse; 1:1000; fig 1f
Abcam UCP1 antibody (AbCam, ab209483) was used in immunohistochemistry on mouse samples (fig 1e) and in western blot on mouse samples at 1:1000 (fig 1f). Oxid Med Cell Longev (2021) ncbi
domestic rabbit monoclonal
  • immunohistochemistry; mouse; loading ...; fig 4d
Abcam UCP1 antibody (Abcam, ab209483) was used in immunohistochemistry on mouse samples (fig 4d). Cells (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2f
  • western blot; mouse; 1:1000; loading ...; fig 1a
Abcam UCP1 antibody (Abcam, ab155117) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 2f) and in western blot on mouse samples at 1:1000 (fig 1a). Redox Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Abcam UCP1 antibody (Abcam, ab155117) was used in western blot on human samples at 1:1000 (fig 3b). PLoS ONE (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Abcam UCP1 antibody (Abcam, ab155117) was used in western blot on human samples at 1:1000 (fig 3b). Stem Cells Int (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5f
Abcam UCP1 antibody (Abcam, ab155117) was used in western blot on mouse samples (fig 5f). Stem Cells Int (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; 1:5000; loading ...; fig 3h
Invitrogen UCP1 antibody (Thermo Fisher Scientific, PA124894) was used in western blot on mouse samples at 1:5000 (fig 3h). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4c
Invitrogen UCP1 antibody (Thermo Fisher Scientific, PA1-24894) was used in western blot on mouse samples at 1:1000 (fig 4c). EMBO Mol Med (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2b
Invitrogen UCP1 antibody (Pierce, PA1-24894) was used in western blot on mouse samples at 1:1000 (fig 2b). J Biol Chem (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2e
In order to report that maternal resveratrol intervention protects offspring against high-fat diet-induced obesity, Invitrogen UCP1 antibody (Thermo Scientific, PA1-24894) was used in western blot on mouse samples at 1:1000 (fig 2e). J Physiol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 1
In order to determine use of coupled and uncoupled energy expenditure pathways by proteomic analysis of human brown adipose tissue, Invitrogen UCP1 antibody (Thermo Fisher, PA1-24894) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 1
In order to research how beige and brown adipose tissue are inhibited in the Gq signalling pathway, Invitrogen UCP1 antibody (Thermo Fisher Scientific, PA1-24894) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
In order to elucidate how the microbiota-fat signaling axis affects beige-fat development, Invitrogen UCP1 antibody (Pierce, PA1- 24894) was used . Nat Med (2015) ncbi
domestic rabbit polyclonal
In order to examine the effect of Roux-en-Y gastric bypass surgery on natriuretic peptides, Invitrogen UCP1 antibody (Fisher Scientific, PA1-24894) was used . Mol Metab (2015) ncbi
domestic rabbit polyclonal
In order to investigate apelin signaling in brown adipocyte tissue, Invitrogen UCP1 antibody (Fisher, PA1-24894) was used . J Biol Chem (2015) ncbi
R&D Systems
mouse monoclonal (536435)
  • immunocytochemistry knockout validation; mouse; 1:300; loading ...; fig 6a
  • flow cytometry; mouse; loading ...; fig 6b
R&D Systems UCP1 antibody (R&D Systems, MAB6158) was used in immunocytochemistry knockout validation on mouse samples at 1:300 (fig 6a) and in flow cytometry on mouse samples (fig 6b). Cell Rep (2018) ncbi
mouse monoclonal (536435)
  • western blot; mouse; loading ...; fig s3f
R&D Systems UCP1 antibody (R&D Systems, MAB6158) was used in western blot on mouse samples (fig s3f). Nature (2017) ncbi
mouse monoclonal (536435)
  • western blot; human; 1:1000; fig 2
R&D Systems UCP1 antibody (R&D Systems, mab6158) was used in western blot on human samples at 1:1000 (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (536435)
  • immunohistochemistry; mouse; 1:1000; fig 1c
  • western blot; mouse; fig 2h
R&D Systems UCP1 antibody (R&D systems, MAB6158) was used in immunohistochemistry on mouse samples at 1:1000 (fig 1c) and in western blot on mouse samples (fig 2h). Mol Metab (2016) ncbi
mouse monoclonal (536435)
  • immunohistochemistry; mouse
R&D Systems UCP1 antibody (R&D Systems, MAB6158) was used in immunohistochemistry on mouse samples . Mol Cell Biol (2014) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 1k
MilliporeSigma UCP1 antibody (Sigma, U6382) was used in western blot on mouse samples at 1:500 (fig 1k). Nat Commun (2018) ncbi
mouse monoclonal (4B7)
  • western blot; rat; 1:500; loading ...; fig 5b
In order to report the effects of dietary calcium supplementation on brown adipose tissue, autonomic nerve activity, sympathoadrenal function, and cardiovascular parameters in adult obese rats that were raised in small litters, MilliporeSigma UCP1 antibody (Sigma Aldrich, SAB1404511) was used in western blot on rat samples at 1:500 (fig 5b). J Nutr Biochem (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b
In order to investigate the differentiation of mesenchymal stem cells into beige/brown adipocytes, MilliporeSigma UCP1 antibody (Sigma, U6382) was used in western blot on human samples (fig 1b). Biochem Biophys Res Commun (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 3a
In order to assess the ability of agrimol B to modulate SIRT1 activity and fat metabolism, MilliporeSigma UCP1 antibody (Sigma, U6382) was used in immunocytochemistry on mouse samples (fig 3a). Biochem Biophys Res Commun (2016) ncbi
domestic rabbit polyclonal
In order to investigate the role of the gustatory signaling pathway in diet-induced obesity, MilliporeSigma UCP1 antibody (Sigma-Aldrich, U6382) was used . PLoS ONE (2015) ncbi
domestic rabbit polyclonal
MilliporeSigma UCP1 antibody (Sigma-Aldrich, U6382) was used . J Endocrinol (2015) ncbi
Articles Reviewed
  1. Pan J, Lin H, Yu J, Zhang H, Zhang J, Wang C, et al. MiR-21-3p Inhibits Adipose Browning by Targeting FGFR1 and Aggravates Atrial Fibrosis in Diabetes. Oxid Med Cell Longev. 2021;2021:9987219 pubmed publisher
  2. Proniewski B, Bar A, Kieronska Rudek A, Suraj Prazmowska J, Buczek E, Czamara K, et al. Systemic Administration of Insulin Receptor Antagonist Results in Endothelial and Perivascular Adipose Tissue Dysfunction in Mice. Cells. 2021;10: pubmed publisher
  3. Mu W, Qian S, Song Y, Yang L, Song S, Yang Q, et al. BMP4-mediated browning of perivascular adipose tissue governs an anti-inflammatory program and prevents atherosclerosis. Redox Biol. 2021;43:101979 pubmed publisher
  4. Galmozzi A, Kok B, Kim A, Montenegro Burke J, Lee J, Spreafico R, et al. PGRMC2 is an intracellular haem chaperone critical for adipocyte function. Nature. 2019;576:138-142 pubmed publisher
  5. Hagberg C, Li Q, Kutschke M, Bhowmick D, Kiss E, Shabalina I, et al. Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity. Cell Rep. 2018;24:2746-2756.e5 pubmed publisher
  6. Bayindir Buchhalter I, Wolff G, Lerch S, Sijmonsma T, Schuster M, Gronych J, et al. Cited4 is a sex-biased mediator of the antidiabetic glitazone response in adipocyte progenitors. EMBO Mol Med. 2018;10: pubmed publisher
  7. Vargas D, Lopez C, Acero E, Benítez E, Wintaco A, Camacho J, et al. Thermogenic capacity of human periaortic adipose tissue is transformed by body weight. PLoS ONE. 2018;13:e0194269 pubmed publisher
  8. Velázquez Villegas L, Perino A, Lemos V, Zietak M, Nomura M, Pols T, et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun. 2018;9:245 pubmed publisher
  9. Lizcano F, Vargas D, Gomez A, Torrado A. Human ADMC-Derived Adipocyte Thermogenic Capacity Is Regulated by IL-4 Receptor. Stem Cells Int. 2017;2017:2767916 pubmed publisher
  10. Emmett M, Lim H, Jager J, Richter H, Adlanmerini M, Peed L, et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature. 2017;546:544-548 pubmed publisher
  11. Song Z, Xiaoli A, Zhang Q, Zhang Y, Yang E, Wang S, et al. Cyclin C regulates adipogenesis by stimulating transcriptional activity of CCAAT/enhancer-binding protein ?. J Biol Chem. 2017;292:8918-8932 pubmed publisher
  12. Zou T, Chen D, Yang Q, Wang B, Zhu M, Nathanielsz P, et al. Resveratrol supplementation of high-fat diet-fed pregnant mice promotes brown and beige adipocyte development and prevents obesity in male offspring. J Physiol. 2017;595:1547-1562 pubmed publisher
  13. Conceição E, Moura E, Oliveira E, Guarda D, Figueiredo M, Quitete F, et al. Dietary calcium supplementation in adult rats reverts brown adipose tissue dysfunction programmed by postnatal early overfeeding. J Nutr Biochem. 2017;39:117-125 pubmed publisher
  14. Wang Y, Lin S, Hsieh P, Hung S. Concomitant beige adipocyte differentiation upon induction of mesenchymal stem cells into brown adipocytes. Biochem Biophys Res Commun. 2016;478:689-95 pubmed publisher
  15. Müller S, Balaz M, Stefanicka P, Varga L, Amri E, Ukropec J, et al. Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways. Sci Rep. 2016;6:30030 pubmed publisher
  16. Wang S, Zhang Q, Zhang Y, Shen C, Wang Z, Wu Q, et al. Agrimol B suppresses adipogenesis through modulation of SIRT1-PPAR gamma signal pathway. Biochem Biophys Res Commun. 2016;477:454-60 pubmed publisher
  17. Zhang C, Weng Y, Shi F, Jin W. The Engrailed-1 Gene Stimulates Brown Adipogenesis. Stem Cells Int. 2016;2016:7369491 pubmed publisher
  18. Klepac K, Kilić A, Gnad T, Brown L, Herrmann B, Wilderman A, et al. The Gq signalling pathway inhibits brown and beige adipose tissue. Nat Commun. 2016;7:10895 pubmed publisher
  19. Lee M, Goralczyk A, Kriszt R, Ang X, Badowski C, Li Y, et al. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Sci Rep. 2016;6:21173 pubmed publisher
  20. Stine R, Shapira S, Lim H, Ishibashi J, Harms M, Won K, et al. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue. Mol Metab. 2016;5:57-65 pubmed publisher
  21. Avau B, Bauters D, Steensels S, Vancleef L, Laermans J, Lesuisse J, et al. The Gustatory Signaling Pathway and Bitter Taste Receptors Affect the Development of Obesity and Adipocyte Metabolism in Mice. PLoS ONE. 2015;10:e0145538 pubmed publisher
  22. Suárez Zamorano N, Fabbiano S, Chevalier C, Stojanović O, Colin D, Stevanović A, et al. Microbiota depletion promotes browning of white adipose tissue and reduces obesity. Nat Med. 2015;21:1497-1501 pubmed publisher
  23. Neinast M, Frank A, Zechner J, Li Q, Vishvanath L, Palmer B, et al. Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning. Mol Metab. 2015;4:427-36 pubmed publisher
  24. Than A, He H, Chua S, Xu D, Sun L, Leow M, et al. Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes. J Biol Chem. 2015;290:14679-91 pubmed publisher
  25. Lisboa P, de Oliveira E, Manhães A, Santos Silva A, Pinheiro C, Younes Rapozo V, et al. Effects of maternal nicotine exposure on thyroid hormone metabolism and function in adult rat progeny. J Endocrinol. 2015;224:315-25 pubmed publisher
  26. Fu T, Seok S, Choi S, Huang Z, Suino Powell K, Xu H, et al. MicroRNA 34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte fibroblast growth factor 21 signaling and SIRT1 function. Mol Cell Biol. 2014;34:4130-42 pubmed publisher