This is a Validated Antibody Database (VAD) review about human UBQLN2, based on 9 published articles (read how Labome selects the articles), using UBQLN2 antibody in all methods. It is aimed to help Labome visitors find the most suited UBQLN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UBQLN2 synonym: ALS15; CHAP1; DSK2; HRIHFB2157; N4BP4; PLIC2

Knockout validation
Novus Biologicals
mouse monoclonal (6H9)
  • western blot knockout validation; human; 1:2000; loading ...; fig 6h
  • immunocytochemistry; human; 1:1000; loading ...; fig 1e
Novus Biologicals UBQLN2 antibody (Novus, NBP2-25164) was used in western blot knockout validation on human samples at 1:2000 (fig 6h) and in immunocytochemistry on human samples at 1:1000 (fig 1e). Cell Mol Life Sci (2022) ncbi
Novus Biologicals
mouse monoclonal (6H9)
  • western blot knockout validation; human; 1:2000; loading ...; fig 6h
  • immunocytochemistry; human; 1:1000; loading ...; fig 1e
Novus Biologicals UBQLN2 antibody (Novus, NBP2-25164) was used in western blot knockout validation on human samples at 1:2000 (fig 6h) and in immunocytochemistry on human samples at 1:1000 (fig 1e). Cell Mol Life Sci (2022) ncbi
mouse monoclonal (6H9)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1, 3
  • western blot; mouse; loading ...; fig s11a, s12a
Novus Biologicals UBQLN2 antibody (Novus, NBP2-25164) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 1, 3) and in western blot on mouse samples (fig s11a, s12a). Brain Pathol (2021) ncbi
Abnova
mouse monoclonal (5F5)
  • immunohistochemistry - paraffin section; human; 1:5000; loading ...; tbl 1
In order to examine eosinophilic neuronal cytoplasmic inclusions, Abnova UBQLN2 antibody (Abnova, 5F5) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (tbl 1). Neuropathology (2016) ncbi
mouse monoclonal (5F5)
  • immunohistochemistry - paraffin section; human; 1:250
Abnova UBQLN2 antibody (Abnova, 5F5) was used in immunohistochemistry - paraffin section on human samples at 1:250. Neurobiol Dis (2015) ncbi
mouse monoclonal (5F5)
  • immunohistochemistry - paraffin section; human; 1:10,000
Abnova UBQLN2 antibody (Abnova, H00029978-M03) was used in immunohistochemistry - paraffin section on human samples at 1:10,000. J Neuropathol Exp Neurol (2015) ncbi
mouse monoclonal (5F5)
  • immunohistochemistry - paraffin section; human; 1:5000
In order to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases, Abnova UBQLN2 antibody (Abnova, 5F5) was used in immunohistochemistry - paraffin section on human samples at 1:5000. Neuropathology (2014) ncbi
Invitrogen
mouse monoclonal (3D5E2)
  • immunohistochemistry; mouse
  • western blot; mouse
In order to evaluate the diagnostic significance of ubiquilin expression in Huntington disease, Invitrogen UBQLN2 antibody (Invitrogen, 37-7700) was used in immunohistochemistry on mouse samples and in western blot on mouse samples . Brain Res (2015) ncbi
mouse monoclonal (3D5E2)
  • immunohistochemistry; mouse; 1:1000
  • western blot; mouse
In order to test if overexpression of ubiquilin-1 delays or prevents neurodegeneration in R6/2 mice, Invitrogen UBQLN2 antibody (Invitrogen, clone 3D5E2) was used in immunohistochemistry on mouse samples at 1:1000 and in western blot on mouse samples . PLoS ONE (2014) ncbi
EnCor Biotechnology
domestic rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 4b
EnCor Biotechnology UBQLN2 antibody (EnCor Biotechnology, RPCA-Ubi-1) was used in western blot on human samples at 1:2000 (fig 4b). Am J Pathol (2018) ncbi
Articles Reviewed
  1. Mohan H, Trzeciakiewicz H, Pithadia A, Crowley E, Pacitto R, Safren N, et al. RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control. Cell Mol Life Sci. 2022;79:176 pubmed publisher
  2. Higgins N, Greenslade J, Wu J, Miranda E, Galliciotti G, Monteiro M. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Brain Pathol. 2021;:e12948 pubmed publisher
  3. Lee S, Bazick H, Chittoor Vinod V, Al Salihi M, Xia G, Notterpek L. Elevated Peripheral Myelin Protein 22, Reduced Mitotic Potential, and Proteasome Impairment in Dermal Fibroblasts from Charcot-Marie-Tooth Disease Type 1A Patients. Am J Pathol. 2018;188:728-738 pubmed publisher
  4. Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  5. Zeng L, Wang B, Merillat S, Minakawa E, Perkins M, Ramani B, et al. Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3. Neurobiol Dis. 2015;82:281-288 pubmed publisher
  6. Porta S, Kwong L, Trojanowski J, Lee V. Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases. J Neuropathol Exp Neurol. 2015;74:380-7 pubmed publisher
  7. Safren N, Chang L, Dziki K, Monteiro M. Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease. Brain Res. 2015;1597:37-46 pubmed publisher
  8. Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  9. Safren N, El Ayadi A, Chang L, Terrillion C, Gould T, BOEHNING D, et al. Ubiquilin-1 overexpression increases the lifespan and delays accumulation of Huntingtin aggregates in the R6/2 mouse model of Huntington's disease. PLoS ONE. 2014;9:e87513 pubmed publisher