This is a Validated Antibody Database (VAD) review about human UBE2I, based on 14 published articles (read how Labome selects the articles), using UBE2I antibody in all methods. It is aimed to help Labome visitors find the most suited UBE2I antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UBE2I synonym: C358B7.1; P18; UBC9; SUMO-conjugating enzyme UBC9; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I

Santa Cruz Biotechnology
mouse monoclonal (C-12)
  • western blot; human; 1:250; loading ...; fig 1c
Santa Cruz Biotechnology UBE2I antibody (Santa Cruz, sc-271057) was used in western blot on human samples at 1:250 (fig 1c). Exp Cell Res (2018) ncbi
mouse monoclonal (C-12)
  • western blot; human; fig 2
Santa Cruz Biotechnology UBE2I antibody (santa Cruz, sc-271057) was used in western blot on human samples (fig 2). PLoS ONE (2016) ncbi
mouse monoclonal (C-12)
  • western blot; human; 1:500; fig st2
In order to study inhibition of growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex by ON 01910.Na, Santa Cruz Biotechnology UBE2I antibody (Santa Cruz, sc-271057) was used in western blot on human samples at 1:500 (fig st2). Transl Res (2016) ncbi
Abcam
rabbit monoclonal (EP2938Y)
  • western blot; human; loading ...; fig 2e
Abcam UBE2I antibody (abcam, ab75854) was used in western blot on human samples (fig 2e). elife (2019) ncbi
rabbit polyclonal
  • western blot; human; fig 1a
Abcam UBE2I antibody (Abcam, ab33044) was used in western blot on human samples (fig 1a). Stem Cell Reports (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1a
  • western blot; human; fig 4f
Abcam UBE2I antibody (Abcam, ab33044) was used in immunocytochemistry on human samples (fig 1a) and in western blot on human samples (fig 4f). Mol Biol Cell (2014) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 1e
In order to assess inhibition of the SUMOylation of VCP that leads to impaired stress response by pathogenic mutations in the valosin-containing protein/p97(VCP) N-domain, Proteintech Group UBE2I antibody (Proteintech, 51018-2-AP) was used in western blot on human samples (fig 1e). J Biol Chem (2016) ncbi
MilliporeSigma
rabbit polyclonal
  • immunocytochemistry; purple sea urchin; 1:100; fig s6
In order to study Nanos localization and stability in the sea urchin, MilliporeSigma UBE2I antibody (Sigma Aldrich, AV43021) was used in immunocytochemistry on purple sea urchin samples at 1:100 (fig s6). Dev Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
In order to report that Salmonella enterica serovar Typhimurium modulates host SUMOylation, MilliporeSigma UBE2I antibody (Sigma, U2634) was used in western blot on human samples (fig 5). Mol Cell Biol (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; fig 7
Cell Signaling Technology UBE2I antibody (Cell Signaling Tech, 4918) was used in western blot on human samples at 1:1000 (fig 7). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1i
Cell Signaling Technology UBE2I antibody (Cell Signaling, 4918) was used in western blot on human samples (fig 1i). Nat Struct Mol Biol (2016) ncbi
rabbit monoclonal (D26F2)
  • western blot; mouse; loading ...; fig 1f
Cell Signaling Technology UBE2I antibody (Cell signaling, 4786) was used in western blot on mouse samples (fig 1f). Nature (2015) ncbi
rabbit monoclonal (D26F2)
  • immunohistochemistry - paraffin section; mouse; 1:100 for IHC, 1:250
  • western blot; mouse; 1:500
In order to profile SUMOylation components during mouse brain development, Cell Signaling Technology UBE2I antibody (Cell Signaling Technology, 4786) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 for IHC, 1:250 and in western blot on mouse samples at 1:500. J Comp Neurol (2014) ncbi
BD Biosciences
mouse monoclonal (50/Ubc9)
  • western blot; human; 1:1000; loading ...; fig 5b
BD Biosciences UBE2I antibody (BD Transduction Lab, 610748) was used in western blot on human samples at 1:1000 (fig 5b). Cell Microbiol (2017) ncbi
Articles Reviewed
  1. Gao K, Li Y, Hu S, Liu Y. SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension. elife. 2019;8: pubmed publisher
  2. Huang Z, Barker D, Gibbins J, Dash P. Talin is a substrate for SUMOylation in migrating cancer cells. Exp Cell Res. 2018;370:417-425 pubmed publisher
  3. Bogachek M, Park J, De Andrade J, Lorenzen A, Kulak M, White J, et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports. 2016;7:1140-1151 pubmed publisher
  4. Malet J, Cossart P, Ribet D. Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. Cell Microbiol. 2017;19: pubmed publisher
  5. Wieczorek E, Kedracka Krok S, Sołtys K, Jankowska U, Hołubowicz R, Seliga J, et al. Is Transthyretin a Regulator of Ubc9 SUMOylation?. PLoS ONE. 2016;11:e0160536 pubmed publisher
  6. Du L, LI Y, Fakih M, Wiatrek R, Duldulao M, Chen Z, et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun. 2016;7:12326 pubmed publisher
  7. Oulhen N, Wessel G. Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin. Dev Biol. 2016;418:146-156 pubmed publisher
  8. Mo Z, Zhang Q, Liu Z, Lauer J, Shi Y, Sun L, et al. Neddylation requires glycyl-tRNA synthetase to protect activated E2. Nat Struct Mol Biol. 2016;23:730-7 pubmed publisher
  9. Wang T, Xu W, Qin M, Yang Y, Bao P, Shen F, et al. Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response. J Biol Chem. 2016;291:14373-84 pubmed publisher
  10. Dai Y, Hung L, Chen R, Lai C, Chang K. ON 01910.Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex. Transl Res. 2016;175:129-143.e13 pubmed publisher
  11. Cheloufi S, Elling U, Hopfgartner B, Jung Y, Murn J, Ninova M, et al. The histone chaperone CAF-1 safeguards somatic cell identity. Nature. 2015;528:218-24 pubmed publisher
  12. Verma S, Mohapatra G, Ahmad S, Rana S, Jain S, Khalsa J, et al. Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival. Mol Cell Biol. 2015;35:2932-46 pubmed publisher
  13. Hasegawa Y, Yoshida D, Nakamura Y, Sakakibara S. Spatiotemporal distribution of SUMOylation components during mouse brain development. J Comp Neurol. 2014;522:3020-36 pubmed publisher
  14. Datta S, Snow C, Paschal B. A pathway linking oxidative stress and the Ran GTPase system in progeria. Mol Biol Cell. 2014;25:1202-15 pubmed publisher