This is a Validated Antibody Database (VAD) review about human UBA1, based on 8 published articles (read how Labome selects the articles), using UBA1 antibody in all methods. It is aimed to help Labome visitors find the most suited UBA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
UBA1 synonym: A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X

Abcam
domestic rabbit monoclonal (EPR14204(B))
  • western blot; human; loading ...; fig e8n
Abcam UBA1 antibody (Abcam, ab181225) was used in western blot on human samples (fig e8n). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7a
In order to discuss targeting UBA1 as a means to treat spinal muscular atrophy, Abcam UBA1 antibody (Abcam, ab34711) was used in western blot on mouse samples at 1:1000 (fig 7a). JCI Insight (2016) ncbi
BioLegend
mouse monoclonal (2G2.3.5)
  • western blot; domestic rabbit; 1:500; fig 6
In order to characterize the role of endolysosomes in skeletal muscle pathology observed in a model of Alzheimer's disease with a cholesterol-fed rabbit, BioLegend UBA1 antibody (Signet, 9320) was used in western blot on domestic rabbit samples at 1:500 (fig 6). Front Aging Neurosci (2016) ncbi
mouse monoclonal (2G2.3.5)
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 3
BioLegend UBA1 antibody (Signet, 9320-02) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 3). Front Neurosci (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
  • western blot; mouse; 1:1000; loading ...; fig 7e
  • western blot; zebrafish ; 1:1000; loading ...; fig 1d
In order to discuss targeting UBA1 as a means to treat spinal muscular atrophy, Invitrogen UBA1 antibody (ThermoFischer, PA5-17274) was used in western blot on human samples at 1:1000 (fig 1a), in western blot on mouse samples at 1:1000 (fig 7e) and in western blot on zebrafish samples at 1:1000 (fig 1d). JCI Insight (2016) ncbi
MilliporeSigma
mouse monoclonal (2G2.3-5)
  • western blot; human; 1:2000; loading ...; fig 2f
MilliporeSigma UBA1 antibody (Sigma-Aldrich, E3152) was used in western blot on human samples at 1:2000 (fig 2f). Nat Cell Biol (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6h
Cell Signaling Technology UBA1 antibody (Cell Signaling Technology, 4890) was used in western blot on human samples (fig 6h). J Clin Invest (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1h
Cell Signaling Technology UBA1 antibody (Cell Signaling, 4891) was used in western blot on human samples (fig 1h). Nat Struct Mol Biol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 6
Cell Signaling Technology UBA1 antibody (Cell Signaling, 4890) was used in western blot on human samples (fig 6). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Wang J, Liu Y, Liu Y, Zheng S, Wang X, Zhao J, et al. Time-resolved protein activation by proximal decaging in living systems. Nature. 2019;569:509-513 pubmed publisher
  2. Li Y, Hu Q, Li C, Liang K, Xiang Y, Hsiao H, et al. PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. J Clin Invest. 2019;129:1129-1151 pubmed publisher
  3. Powis R, Karyka E, Boyd P, Côme J, Jones R, Zheng Y, et al. Systemic restoration of UBA1 ameliorates disease in spinal muscular atrophy. JCI Insight. 2016;1:e87908 pubmed publisher
  4. Chen X, Wagener J, Ghribi O, Geiger J. Role of Endolysosomes in Skeletal Muscle Pathology Observed in a Cholesterol-Fed Rabbit Model of Alzheimer's Disease. Front Aging Neurosci. 2016;8:129 pubmed publisher
  5. Mo Z, Zhang Q, Liu Z, Lauer J, Shi Y, Sun L, et al. Neddylation requires glycyl-tRNA synthetase to protect activated E2. Nat Struct Mol Biol. 2016;23:730-7 pubmed publisher
  6. Löffler T, Flunkert S, Temmel M, Hutter Paier B. Decreased Plasma A? in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front Neurosci. 2016;10:232 pubmed publisher
  7. Lee J, Takahama S, Zhang G, Tomarev S, Ye Y. Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells. Nat Cell Biol. 2016;18:765-76 pubmed publisher
  8. Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher