This is a Validated Antibody Database (VAD) review about human U2AF1, based on 6 published articles (read how Labome selects the articles), using U2AF1 antibody in all methods. It is aimed to help Labome visitors find the most suited U2AF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
U2AF1 synonym: FP793; RNU2AF1; U2AF35; U2AFBP; splicing factor U2AF 35 kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; splicing factor U2AF 35kDa subunit

Proteintech Group
rabbit polyclonal
  • western blot; human; fig s1
Proteintech Group U2AF1 antibody (Proteintech, 10334-1-AP) was used in western blot on human samples (fig s1). Nucleic Acids Res (2017) ncbi
mouse monoclonal (2B7F11)
  • western blot; human; fig s2c
Proteintech Group U2AF1 antibody (Proteintech, 60289) was used in western blot on human samples (fig s2c). Nucleic Acids Res (2017) ncbi
rabbit polyclonal
  • western blot; human
Proteintech Group U2AF1 antibody (Protein Tech Group, 10334-1-AP) was used in western blot on human samples . Nucleic Acids Res (2015) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl U2AF1 antibody (Bethyl, A302-079A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3
Bethyl U2AF1 antibody (Bethyl, A302-079A-1) was used in western blot on human samples (fig 3). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
In order to test how mutations affecting the 3' splice site recognition factor of U2AF1 alter its ability to splice RNA, Bethyl U2AF1 antibody (Bethyl Laboratories, A302-080A) was used in western blot on human samples at 1:1000 (fig 2). Genome Res (2015) ncbi
rabbit polyclonal
  • western blot; human
Bethyl U2AF1 antibody (Bethyl, A307-079A) was used in western blot on human samples . Mol Cell (2014) ncbi
Articles Reviewed
  1. Chen L, Weinmeister R, Kralovicova J, Eperon L, Vorechovsky I, Hudson A, et al. Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. Nucleic Acids Res. 2017;45:2051-2067 pubmed publisher
  2. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  3. Whisenant T, Peralta E, Aarreberg L, Gao N, Head S, Ordoukhanian P, et al. The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells. PLoS ONE. 2015;10:e0144409 pubmed publisher
  4. Kralovicova J, Knut M, Cross N, Vorechovsky I. Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins. Nucleic Acids Res. 2015;43:3747-63 pubmed publisher
  5. Ilagan J, Ramakrishnan A, Hayes B, Murphy M, Zebari A, Bradley P, et al. U2AF1 mutations alter splice site recognition in hematological malignancies. Genome Res. 2015;25:14-26 pubmed publisher
  6. Savage K, Gorski J, Barros E, Irwin G, Manti L, Powell A, et al. Identification of a BRCA1-mRNA splicing complex required for efficient DNA repair and maintenance of genomic stability. Mol Cell. 2014;54:445-59 pubmed publisher