This is a Validated Antibody Database (VAD) review about human TTK, based on 17 published articles (read how Labome selects the articles), using TTK antibody in all methods. It is aimed to help Labome visitors find the most suited TTK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TTK synonym: CT96; ESK; MPH1; MPS1; MPS1L1; PYT; dual specificity protein kinase TTK; cancer/testis antigen 96; monopolar spindle 1 kinase; monopolar spindle 1-like 1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase

Knockout validation
EMD Millipore
mouse monoclonal (3-472-1)
  • western blot knockout validation; mouse; fig 2
EMD Millipore TTK antibody (Millipore, 05-682) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
EMD Millipore
mouse monoclonal (4-112-3)
  • western blot knockout validation; mouse; fig 2
EMD Millipore TTK antibody (Millipore, 05-683) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; mouse; fig 2
Sigma-Aldrich TTK antibody (Sigma, HPA016834) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
Sigma-Aldrich
mouse monoclonal (3G7)
  • western blot knockout validation; mouse; fig 2
Sigma-Aldrich TTK antibody (Sigma, WH0007272M1) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
Invitrogen
mouse monoclonal (N1)
  • western blot; human; loading ...; fig 1b
In order to assess the effects of CCT271850 on MPS1 kinase activity in cancer cells, Invitrogen TTK antibody (Invitrogen, 35-9100) was used in western blot on human samples (fig 1b). Br J Cancer (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to assess Mps1 kinase inhibitors as cancer treatments, Invitrogen TTK antibody (Life Technologies, 44-1325G) was used in immunocytochemistry on human samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1
In order to identify mutations in the kinase domain of MPS1 that confer resistance against multiple inhibitors, Invitrogen TTK antibody (Life Technologies, 44-1325G) was used in immunocytochemistry on human samples (fig 1). Cancer Res (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to study the structure of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1), Invitrogen TTK antibody (Invitrogen, 44-1325G) was used in immunocytochemistry on human samples . J Med Chem (2013) ncbi
mouse monoclonal (N1)
  • immunocytochemistry; human
In order to study the structure of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1), Invitrogen TTK antibody (Invitrogen, 35-9100) was used in immunocytochemistry on human samples . J Med Chem (2013) ncbi
mouse monoclonal (N1)
  • immunohistochemistry - paraffin section; human; fig 1
In order to characterize nonsense-mediated decay-escaping frameshift mutations in microsatellite-unstable colorectal cancers, Invitrogen TTK antibody (Zymed, 35-9100) was used in immunohistochemistry - paraffin section on human samples (fig 1). Carcinogenesis (2011) ncbi
Abcam
mouse monoclonal (N1)
  • western blot; human; fig 5e
Abcam TTK antibody (Abcam, ab11108) was used in western blot on human samples (fig 5e). Biochim Biophys Acta (2016) ncbi
mouse monoclonal (N1)
  • western blot; human; fig 5
Abcam TTK antibody (Abcam, ab11108) was used in western blot on human samples (fig 5). Cell Rep (2016) ncbi
mouse monoclonal (N1)
  • western blot; human
Abcam TTK antibody (Abcam, ab11108) was used in western blot on human samples . Genetics (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (N1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology TTK antibody (SCBT, N1) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human
Cell Signaling Technology TTK antibody (Cell Signaling, 3255S) was used in western blot on human samples . Cell Div (2014) ncbi
EMD Millipore
mouse monoclonal (3-472-1)
  • western blot; human; loading ...; fig 1d
EMD Millipore TTK antibody (Millipore, 05-682) was used in western blot on human samples (fig 1d). Theranostics (2018) ncbi
mouse monoclonal (3-472-1)
  • western blot; human; loading ...; fig s2b
EMD Millipore TTK antibody (Millipore, 05?C682) was used in western blot on human samples (fig s2b). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (4-112-3)
  • western blot knockout validation; mouse; fig 2
EMD Millipore TTK antibody (Millipore, 05-683) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
mouse monoclonal (3-472-1)
  • western blot knockout validation; mouse; fig 2
EMD Millipore TTK antibody (Millipore, 05-682) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
mouse monoclonal (3-472-1)
  • immunocytochemistry; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4c
EMD Millipore TTK antibody (Millipore, 05-682) was used in immunocytochemistry on human samples (fig 4a) and in western blot on human samples (fig 4c). Oncotarget (2015) ncbi
mouse monoclonal (3-472-1)
  • immunocytochemistry; human; fig 1
In order to identify mutations in the kinase domain of MPS1 that confer resistance against multiple inhibitors, EMD Millipore TTK antibody (Millipore, 05-682) was used in immunocytochemistry on human samples (fig 1). Cancer Res (2015) ncbi
mouse monoclonal (4-112-3)
  • western blot; human; 1:1000; loading ...; fig 2a
EMD Millipore TTK antibody (Millipore, 05-683) was used in western blot on human samples at 1:1000 (fig 2a). Oncotarget (2016) ncbi
mouse monoclonal (3-472-1)
  • western blot; human
EMD Millipore TTK antibody (Millipore, 05-682) was used in western blot on human samples . Br J Cancer (2014) ncbi
mouse monoclonal (3-472-1)
  • immunocytochemistry; human; 1:100
EMD Millipore TTK antibody (EMD Millipore, 05-682) was used in immunocytochemistry on human samples at 1:100. J Cell Biol (2014) ncbi
Sigma-Aldrich
mouse monoclonal (3G7)
  • western blot knockout validation; mouse; fig 2
Sigma-Aldrich TTK antibody (Sigma, WH0007272M1) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; fig 2
Sigma-Aldrich TTK antibody (Sigma, HPA016834) was used in western blot knockout validation on mouse samples (fig 2). PLoS Genet (2016) ncbi
Articles Reviewed
  1. Chen X, Yu C, Gao J, Zhu H, Cui B, Zhang T, et al. A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer. Theranostics. 2018;8:2348-2360 pubmed publisher
  2. Faisal A, Mak G, Gurden M, Xavier C, Anderhub S, Innocenti P, et al. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy. Br J Cancer. 2017;116:1166-1176 pubmed publisher
  3. Mason J, Wei X, Fletcher G, Kiarash R, Brokx R, Hodgson R, et al. Functional characterization of CFI-402257, a potent and selective Mps1/TTK kinase inhibitor, for the treatment of cancer. Proc Natl Acad Sci U S A. 2017;114:3127-3132 pubmed publisher
  4. Diril M, Bisteau X, Kitagawa M, Caldez M, Wee S, Gunaratne J, et al. Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet. 2016;12:e1006310 pubmed publisher
  5. Kim J, Ki S, Joung J, Scott E, Heynen Genel S, Aza Blanc P, et al. Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation. Biochim Biophys Acta. 2016;1863:1307-18 pubmed publisher
  6. Wild T, Larsen M, Narita T, Schou J, Nilsson J, Choudhary C. The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity. Cell Rep. 2016;14:1829-40 pubmed publisher
  7. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  8. Zhang P, Zhang Y, Gao K, Wang Y, Jin X, Wei Y, et al. ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. Oncotarget. 2015;6:41550-65 pubmed publisher
  9. Martinez R, Blasina A, Hallin J, Hu W, Rymer I, Fan J, et al. Mitotic Checkpoint Kinase Mps1 Has a Role in Normal Physiology which Impacts Clinical Utility. PLoS ONE. 2015;10:e0138616 pubmed publisher
  10. Bailey M, Singh T, Mero P, Moffat J, Hieter P. Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint. Genetics. 2015;201:885-95 pubmed publisher
  11. Gurden M, Westwood I, Faisal A, Naud S, Cheung K, McAndrew C, et al. Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance. Cancer Res. 2015;75:3340-54 pubmed publisher
  12. Maachani U, Tandle A, Shankavaram U, Kramp T, Camphausen K. Modulation of miR-21 signaling by MPS1 in human glioblastoma. Oncotarget. 2016;7:52912-52927 pubmed publisher
  13. Lee M, Marina M, King J, Saavedra H. Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells. Cell Div. 2014;9:3 pubmed publisher
  14. Kaistha B, Honstein T, Muller V, Bielak S, Sauer M, Kreider R, et al. Key role of dual specificity kinase TTK in proliferation and survival of pancreatic cancer cells. Br J Cancer. 2014;111:1780-7 pubmed publisher
  15. Ballister E, Riegman M, Lampson M. Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. J Cell Biol. 2014;204:901-8 pubmed publisher
  16. Naud S, Westwood I, Faisal A, Sheldrake P, Bavetsias V, Atrash B, et al. Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1). J Med Chem. 2013;56:10045-65 pubmed publisher
  17. Niittymäki I, Gylfe A, Laine L, Laakso M, Lehtonen H, Kondelin J, et al. High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle assembly checkpoint. Carcinogenesis. 2011;32:305-11 pubmed publisher