TSC2 antibody | antibody review based on formal publications

This is a Validated Antibody Database (VAD) review about human TSC2, based on 66 published articles (read how Labome selects the articles), using TSC2 antibody in all methods. It is aimed to help Labome visitors find the most suited TSC2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.

TSC2 synonym: LAM; PPP1R160; TSC4

Knockout validation

Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; loading ...; fig 3g	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig 3g). Cell Death Dis (2021) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; human; loading ...; fig 3e	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot knockout validation on human samples (fig 3e). Nat Commun (2021) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; 1:1000; loading ...; fig 4j	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4j). EMBO Mol Med (2019) ncbi
Cell Signaling Technology domestic rabbit polyclonal 3612	western blot knockout validation; mouse; 1:1000; loading ...; fig 2e western blot; rat; 1:1000; loading ...; fig 1i	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3612) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2e) and in western blot on rat samples at 1:1000 (fig 1i). Nature (2019) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; loading ...; fig 1a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig 1a). J Biol Chem (2017) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; human; 1:1000; loading ...; fig s3h	In order to elucidate that mTORC1 presents tumor suppressor features in conditions of nutrient restrictions, Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on human samples at 1:1000 (fig s3h). Nat Commun (2017) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	immunoprecipitation; rat; loading ...; fig 4c western blot; rat; loading ...; fig 4c western blot knockout validation; human; loading ...; fig 1a immunoprecipitation; human; loading ...; fig 2c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in immunoprecipitation on rat samples (fig 4c), in western blot on rat samples (fig 4c), in western blot knockout validation on human samples (fig 1a) and in immunoprecipitation on human samples (fig 2c). Biochim Biophys Acta Mol Cell Res (2017) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; 1:2000; loading ...; fig s10a immunocytochemistry; mouse; 1:200; loading ...; fig s6b immunocytochemistry; human; 1:200; loading ...; fig s4a western blot; human; 1:2000; loading ...; fig s9a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples at 1:2000 (fig s10a), in immunocytochemistry on mouse samples at 1:200 (fig s6b), in immunocytochemistry on human samples at 1:200 (fig s4a) and in western blot on human samples at 1:2000 (fig s9a). Nat Commun (2016) ncbi
Cell Signaling Technology domestic rabbit monoclonal (28A7) 3635	western blot knockout validation; mouse; 1:1000; fig 1	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3635) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Nat Cell Biol (2016) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot; human; 1:2000; fig s1 western blot knockout validation; mouse; 1:2000; fig 3 immunocytochemistry; mouse; fig 5	Cell Signaling Technology TSC2 antibody (Cell signaling, 4308) was used in western blot on human samples at 1:2000 (fig s1), in western blot knockout validation on mouse samples at 1:2000 (fig 3) and in immunocytochemistry on mouse samples (fig 5). Sci Rep (2015) ncbi
Cell Signaling Technology domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; fig s1 western blot; human; fig s1	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig s1) and in western blot on human samples (fig s1). Cell (2014) ncbi

Invitrogen

domestic rabbit monoclonal (T.309.0) MA5-15004	western blot; human; loading ...; fig s5j	In order to study the role of the PTEN-PI3K-mTORC1 pathway in polyamine metabolism in cancer cells, Invitrogen TSC2 antibody (Thermo Scientific, MA5-15004) was used in western blot on human samples (fig s5j). Nature (2017) ncbi
domestic rabbit monoclonal (T.309.0) MA5-15004	western blot; human; 1:1000; fig s7	In order to elucidate the mechanisms by which mutant IDH1/2 contributes to oncogenesis, Invitrogen TSC2 antibody (Thermo Scientific, T.309-0) was used in western blot on human samples at 1:1000 (fig s7). Nat Commun (2016) ncbi
mouse monoclonal (3G9D9) 37-0500	immunoprecipitation; human	In order to identify molecular targets that suppress tumorigenesis in triple negative breast cancers, Invitrogen TSC2 antibody (Life Technology, 37-0500) was used in immunoprecipitation on human samples . J Clin Invest (2015) ncbi
mouse monoclonal (3G9D9) 37-0500	immunohistochemistry - paraffin section; human; 1:20; tbl 1	In order to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene, Invitrogen TSC2 antibody (Zymed, 3G9D9) was used in immunohistochemistry - paraffin section on human samples at 1:20 (tbl 1). Neuropathology (2008) ncbi

Abcam

domestic rabbit monoclonal (Y320) ab32554	western blot; human; 1:1000; loading ...; fig 6d	Abcam TSC2 antibody (Abcam, ab32554) was used in western blot on human samples at 1:1000 (fig 6d). Int J Biol Sci (2019) ncbi
domestic rabbit monoclonal (Y320) ab32554	other; human; loading ...; fig 4c	Abcam TSC2 antibody (Abcam, ab32554) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (EPR8202) ab133465	western blot; mouse; 1:1000; fig 4	Abcam TSC2 antibody (Abcam, ab133465) was used in western blot on mouse samples at 1:1000 (fig 4). Sci Rep (2015) ncbi

Santa Cruz Biotechnology

mouse monoclonal (325.Ser 1798)

sc-293149

western blot; human

Santa Cruz Biotechnology TSC2 antibody (Santa Cruz Biotechnology, sc-293149) was used in western blot on human samples . Cell Signal (2015) ncbi

Cell Signaling Technology

domestic rabbit monoclonal (D93F12) 4308	western blot; human; loading ...; fig 4a, 4b	Cell Signaling Technology TSC2 antibody (CST, 4308) was used in western blot on human samples (fig 4a, 4b). Molecules (2022) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:1000; loading ...; fig 1c, s1a, 4d	Cell Signaling Technology TSC2 antibody (CST, 4308) was used in western blot on mouse samples at 1:1000 (fig 1c, s1a, 4d). Cell Rep (2022) ncbi
domestic rabbit polyclonal 3612	other; mouse; loading ...; fig 6d, 7d	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3612) was used in other on mouse samples (fig 6d, 7d). Autophagy (2022) ncbi
domestic rabbit monoclonal (28A7) 3635	western blot; human; 1:1000; fig 1a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3635) was used in western blot on human samples at 1:1000 (fig 1a). Biomedicines (2021) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; loading ...; fig 2a, 3b	Cell Signaling Technology TSC2 antibody (Cell signaling, 4308) was used in western blot on mouse samples (fig 2a, 3b). Mol Metab (2022) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; loading ...	Cell Signaling Technology TSC2 antibody (Cell signaling technology, 4308) was used in western blot on human samples . Nat Commun (2021) ncbi
domestic rabbit polyclonal 3611	western blot; human; loading ...	Cell Signaling Technology TSC2 antibody (Cell signaling technology, 3611) was used in western blot on human samples . Nat Commun (2021) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; loading ...; fig 3g	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig 3g). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; human; loading ...; fig 3e	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot knockout validation on human samples (fig 3e). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:1000; loading ...; fig s12	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on mouse samples at 1:1000 (fig s12). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal 5584	western blot; mouse; 1:1000; loading ...; fig 5c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 5584) was used in western blot on mouse samples at 1:1000 (fig 5c). Aging Cell (2021) ncbi
domestic rabbit polyclonal 3612	immunocytochemistry; human; loading ...; fig 2b western blot; human; loading ...; fig 2a	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3612) was used in immunocytochemistry on human samples (fig 2b) and in western blot on human samples (fig 2a). Sci Adv (2021) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; loading ...; fig 4a	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308S) was used in western blot on human samples (fig 4a). Cancer Res (2020) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; 1:1000; loading ...; fig 4j	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4j). EMBO Mol Med (2019) ncbi
domestic rabbit polyclonal 3615	western blot; human; 1:1000; loading ...; fig 6d	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3615) was used in western blot on human samples at 1:1000 (fig 6d). Int J Biol Sci (2019) ncbi
domestic rabbit polyclonal 5584	western blot; mouse; 1:1000; loading ...; fig 2e	Cell Signaling Technology TSC2 antibody (Cell Signaling, 5584) was used in western blot on mouse samples at 1:1000 (fig 2e). Nature (2019) ncbi
domestic rabbit polyclonal 3612	western blot knockout validation; mouse; 1:1000; loading ...; fig 2e western blot; rat; 1:1000; loading ...; fig 1i	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3612) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2e) and in western blot on rat samples at 1:1000 (fig 1i). Nature (2019) ncbi
domestic rabbit polyclonal 3612	immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig 2b	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3612) was used in immunohistochemistry - frozen section on mouse samples at 1:2000 (fig 2b). Physiol Rep (2019) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; loading ...; fig 2a	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot on human samples (fig 2a). Autophagy (2019) ncbi
domestic rabbit monoclonal (5B12) 3617	other; human; loading ...; fig 4c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal 5584	western blot; mouse; loading ...; fig 3d	Cell Signaling Technology TSC2 antibody (Cell Signaling, 5584) was used in western blot on mouse samples (fig 3d). Biochem Biophys Res Commun (2018) ncbi
domestic rabbit polyclonal 3612	western blot; mouse; loading ...; fig 3d	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3612) was used in western blot on mouse samples (fig 3d). Biochem Biophys Res Commun (2018) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; mouse; loading ...; fig s1	In order to research the role of tuberous sclerosis complex 1 in epilepsy and its mechanism, Cell Signaling Technology TSC2 antibody (Cell Signaling, 3990) was used in western blot on mouse samples (fig s1). Epilepsia (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; 1:1000; loading ...; fig 3c	In order to research the effect of methyl-beta-cyclodextrin on autophagy flux in Niemann-Pick C1-deficient cells, Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308s) was used in western blot on human samples at 1:1000 (fig 3c). Autophagy (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	immunocytochemistry; human; 1:800; loading ...; fig 5g western blot; human; 1:1000; loading ...; fig 5i	In order to research the roles of WIPI3 and WIPI4 in autophagy, Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in immunocytochemistry on human samples at 1:800 (fig 5g) and in western blot on human samples at 1:1000 (fig 5i). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; loading ...; fig 5c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on human samples (fig 5c). Sci Rep (2017) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; loading ...; fig 5c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on human samples (fig 5c). Sci Rep (2017) ncbi
domestic rabbit monoclonal (5B12) 3617	reverse phase protein lysate microarray; human; loading ...; fig st6	In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology TSC2 antibody (CST, 3617) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; loading ...; fig 1a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig 1a). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; rat; loading ...; fig 4c	In order to probe the role of mTOR-dependent signaling among neuronal and nonneuronal cells in myelin regulation, Cell Signaling Technology TSC2 antibody (Cell Signaling, D93F12) was used in western blot on rat samples (fig 4c). J Exp Med (2017) ncbi
domestic rabbit monoclonal (5B12) 3617	reverse phase protein lysate microarray; human; loading ...; fig 3a	In order to describe the features of 228 primary cervical cancers, Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; human; 1:1000; loading ...; fig s3h	In order to elucidate that mTORC1 presents tumor suppressor features in conditions of nutrient restrictions, Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on human samples at 1:1000 (fig s3h). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; loading ...; fig 1b	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on mouse samples (fig 1b). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; mouse; loading ...; fig 4a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on mouse samples (fig 4a). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; loading ...; fig 6b	In order to confirm that FBXL5 contributes to regulation of neural stem-progenitor cells proliferation during mammalian brain development, Cell Signaling Technology TSC2 antibody (CST, 4308) was used in western blot on mouse samples (fig 6b). Mol Cell Biol (2017) ncbi
domestic rabbit polyclonal 3616	western blot; mouse; loading ...; fig 2a	In order to assess the role of p38 signaling in limiting regulatory T cell induction, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3616) was used in western blot on mouse samples (fig 2a). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; human; fig 6a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3990) was used in western blot on human samples (fig 6a). Cell (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	immunoprecipitation; rat; loading ...; fig 4c western blot; rat; loading ...; fig 4c western blot knockout validation; human; loading ...; fig 1a immunoprecipitation; human; loading ...; fig 2c	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in immunoprecipitation on rat samples (fig 4c), in western blot on rat samples (fig 4c), in western blot knockout validation on human samples (fig 1a) and in immunoprecipitation on human samples (fig 2c). Biochim Biophys Acta Mol Cell Res (2017) ncbi
domestic rabbit polyclonal 3612	western blot; human; fig 1a	Cell Signaling Technology TSC2 antibody (cell signalling, 3612) was used in western blot on human samples (fig 1a). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; loading ...; fig 8b	Cell Signaling Technology TSC2 antibody (Cell Signaling, 5B12) was used in western blot on human samples (fig 8b). Oncotarget (2016) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; mouse; loading ...; fig 3b	In order to propose that PTEN's role in axonal polarity can be independent of PIP3, Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on mouse samples (fig 3b). Biochim Biophys Acta (2016) ncbi
domestic rabbit polyclonal 3612	western blot; mouse; loading ...; fig 3b	In order to propose that PTEN's role in axonal polarity can be independent of PIP3, Cell Signaling Technology TSC2 antibody (Cell Signaling, 93612) was used in western blot on mouse samples (fig 3b). Biochim Biophys Acta (2016) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; 1:1000; fig 4	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
domestic rabbit polyclonal 5584	western blot; human; 1:1000; fig 4	Cell Signaling Technology TSC2 antibody (Cell Signaling, 5584) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; 1:1000; fig 4 western blot; mouse; 1:1000; fig 4	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on human samples at 1:1000 (fig 4) and in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:1000; fig 3	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on mouse samples at 1:1000 (fig 3). Hum Mol Genet (2016) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; mouse; 1:1000; fig 3	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on mouse samples at 1:1000 (fig 3). Hum Mol Genet (2016) ncbi
domestic rabbit polyclonal 3611	western blot; mouse; 1:1000; loading ...; fig s10b	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3611) was used in western blot on mouse samples at 1:1000 (fig s10b). Nat Commun (2016) ncbi
domestic rabbit polyclonal 3615	western blot; mouse; 1:1000; loading ...; fig s10b	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3615) was used in western blot on mouse samples at 1:1000 (fig s10b). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; 1:2000; loading ...; fig s10a immunocytochemistry; mouse; 1:200; loading ...; fig s6b immunocytochemistry; human; 1:200; loading ...; fig s4a western blot; human; 1:2000; loading ...; fig s9a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples at 1:2000 (fig s10a), in immunocytochemistry on mouse samples at 1:200 (fig s6b), in immunocytochemistry on human samples at 1:200 (fig s4a) and in western blot on human samples at 1:2000 (fig s9a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (28A7) 3635	western blot knockout validation; mouse; 1:1000; fig 1	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3635) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Nat Cell Biol (2016) ncbi
domestic rabbit polyclonal 3612	western blot; mouse; fig 1a	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3612) was used in western blot on mouse samples (fig 1a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; mouse; fig 5	Cell Signaling Technology TSC2 antibody (Cell signaling, 3617) was used in western blot on mouse samples (fig 5). Cell Death Dis (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	immunocytochemistry; human; 1:1000; fig 2a western blot; human; 1:1000; fig 2e immunocytochemistry; mouse; 1:1000; fig 2c western blot; mouse; 1:1000; fig 1s3i	In order to survey mTORC1 activity reguled by control of TSC2-Rheb signaling by arginine, Cell Signaling Technology TSC2 antibody (Cell Signaling Technologies, 4308) was used in immunocytochemistry on human samples at 1:1000 (fig 2a), in western blot on human samples at 1:1000 (fig 2e), in immunocytochemistry on mouse samples at 1:1000 (fig 2c) and in western blot on mouse samples at 1:1000 (fig 1s3i). elife (2016) ncbi
domestic rabbit polyclonal 3615	western blot; human; 1:1000; fig 1s3h western blot; mouse; 1:1000; fig 1s3i	In order to survey mTORC1 activity reguled by control of TSC2-Rheb signaling by arginine, Cell Signaling Technology TSC2 antibody (Cell Signaling Technologies, 3615S) was used in western blot on human samples at 1:1000 (fig 1s3h) and in western blot on mouse samples at 1:1000 (fig 1s3i). elife (2016) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; fig 5 western blot; mouse; fig 5	Cell Signaling Technology TSC2 antibody (Cell signaling, 5B12) was used in western blot on human samples (fig 5) and in western blot on mouse samples (fig 5). J Immunol (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; fig 5 western blot; mouse; fig 5	Cell Signaling Technology TSC2 antibody (Cell signaling, D93F12) was used in western blot on human samples (fig 5) and in western blot on mouse samples (fig 5). J Immunol (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:1000; fig 2b	In order to determine how reuglations of m TORC1 signalling occurs by SUMOylation of AMPK alpha 1 through PIAS4, Cell Signaling Technology TSC2 antibody (Cell Signaling technology, 4308) was used in western blot on mouse samples at 1:1000 (fig 2b). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; fig 2	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on human samples (fig 2). Sci Rep (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:2500; fig 5	In order to characterize TGF Beta1-induced apoptosis in podocytes via extracellular signal-regulated kinase-mammalian target of rapamycin complex 1-NADPH oxidase 4 axis, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot on mouse samples at 1:2500 (fig 5). J Biol Chem (2015) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; mouse; 1:2000; fig 5	In order to characterize TGF Beta1-induced apoptosis in podocytes via extracellular signal-regulated kinase-mammalian target of rapamycin complex 1-NADPH oxidase 4 axis, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3617) was used in western blot on mouse samples at 1:2000 (fig 5). J Biol Chem (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	immunocytochemistry; human; loading ...; fig 5d	Cell Signaling Technology TSC2 antibody (Cell Signalling, 4308) was used in immunocytochemistry on human samples (fig 5d). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; human; fig 6	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3990S) was used in western blot on human samples (fig 6). Mol Cancer Res (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	immunocytochemistry; human; 1:800; fig 5	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in immunocytochemistry on human samples at 1:800 (fig 5). Mol Cancer Res (2016) ncbi
domestic rabbit polyclonal 5584	western blot; human; fig 3	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 5584S) was used in western blot on human samples (fig 3). Mol Cancer Res (2016) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; 1:2000; fig s1 western blot knockout validation; mouse; 1:2000; fig 3 immunocytochemistry; mouse; fig 5	Cell Signaling Technology TSC2 antibody (Cell signaling, 4308) was used in western blot on human samples at 1:2000 (fig s1), in western blot knockout validation on mouse samples at 1:2000 (fig 3) and in immunocytochemistry on mouse samples (fig 5). Sci Rep (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; fig 5	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on human samples (fig 5). Nat Immunol (2015) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; fig 5	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on human samples (fig 5). Nat Immunol (2015) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; human	In order to identify molecular targets that suppress tumorigenesis in triple negative breast cancers, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3990) was used in western blot on human samples . J Clin Invest (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human; 1:1000	Cell Signaling Technology TSC2 antibody (Cell Signalling, 4308) was used in western blot on human samples at 1:1000. Sci Rep (2015) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; fig s1	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on human samples (fig s1). Cell Death Dis (2015) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; mouse; fig s3	In order to characterize the modulation of lysosomal function and autophagy due to CAV1/caveolin-1 in human breast cancer cells after cell stress response, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 3990s) was used in western blot on mouse samples (fig s3). Autophagy (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; rat; 1:1000	Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot on rat samples at 1:1000. Neurobiol Dis (2015) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human	Cell Signaling Technology TSC2 antibody (Cell Signaling Technologies, 3617) was used in western blot on human samples . J Biol Chem (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; human	Cell Signaling Technology TSC2 antibody (Cell Signaling Technologies, 4308) was used in western blot on human samples . J Biol Chem (2015) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; fig 5	In order to investigate if and how mTOR regulates YAP, Cell Signaling Technology TSC2 antibody (Cell Signaling Technology, 4308) was used in western blot on mouse samples (fig 5). J Exp Med (2014) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot knockout validation; mouse; fig s1 western blot; human; fig s1	Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot knockout validation on mouse samples (fig s1) and in western blot on human samples (fig s1). Cell (2014) ncbi
domestic rabbit monoclonal (D93F12) 4308	western blot; mouse; 1:5000; fig 2	In order to determine how FMRP S499 is phosphorylated separately from mTORC1-S6K1 activity, Cell Signaling Technology TSC2 antibody (Cell Signaling, 4308) was used in western blot on mouse samples at 1:5000 (fig 2). PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D57A9) 3990	western blot; mouse; fig 2	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3990) was used in western blot on mouse samples (fig 2). Autophagy (2014) ncbi
domestic rabbit monoclonal (5B12) 3617	western blot; human; fig 2	Cell Signaling Technology TSC2 antibody (Cell Signaling, 3617) was used in western blot on human samples (fig 2). J Physiol (2013) ncbi

Articles Reviewed

Ye G, Xu M, Shu Y, Sun X, Mai Y, Hong Y, et al. A Quassinoid Diterpenoid Eurycomanone from Eurycoma longifolia Jack Exerts Anti-Cancer Effect through Autophagy Inhibition. Molecules. 2022;27: pubmed publisher
Schr xf6 tter S, Yuskaitis C, MacArthur M, Mitchell S, Hosios A, Osipovich M, et al. The non-essential TSC complex component TBC1D7 restricts tissue mTORC1 signaling and brain and neuron growth. Cell Rep. 2022;39:110824 pubmed publisher
Vessey K, Jobling A, Tran M, Wang A, Greferath U, Fletcher E. Treatments targeting autophagy ameliorate the age-related macular degeneration phenotype in mice lacking APOE (apolipoprotein E). Autophagy. 2022;18:2368-2384 pubmed publisher
Ancona S, Orpianesi E, Bernardelli C, Chiaramonte E, Chiaramonte R, Terraneo S, et al. Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM/TSC Cells. Biomedicines. 2021;9: pubmed publisher
Han H, Kim S, Kim Y, Jang S, Kwon Y, Choi D, et al. A novel role of CRTC2 in promoting nonalcoholic fatty liver disease. Mol Metab. 2022;55:101402 pubmed publisher
Yuan T, Annamalai K, Naik S, Lupse B, Geravandi S, Pal A, et al. The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis. Nat Commun. 2021;12:4928 pubmed publisher
Chen X, Miao M, Zhou M, Chen J, Li D, Zhang L, et al. Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway. Cell Death Dis. 2021;12:761 pubmed publisher
Alesi N, Akl E, Khabibullin D, Liu H, Nidhiry A, Garner E, et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021;12:4245 pubmed publisher
Zhang T, He M, Zhao L, Qin S, Zhu Z, Du X, et al. HDAC6 regulates primordial follicle activation through mTOR signaling pathway. Cell Death Dis. 2021;12:559 pubmed publisher
Wallace M, Aguirre N, Marcotte G, Marshall A, Baehr L, Hughes D, et al. The ketogenic diet preserves skeletal muscle with aging in mice. Aging Cell. 2021;20:e13322 pubmed publisher
Cheah P, Prabhakar S, Yellen D, Beauchamp R, Zhang X, Kasamatsu S, et al. Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin. Sci Adv. 2021;7: pubmed publisher
Feng Y, Mischler W, Gurung A, Kavanagh T, Androsov G, Sadow P, et al. Therapeutic Targeting of the Secreted Lysophospholipase D Autotaxin Suppresses Tuberous Sclerosis Complex-Associated Tumorigenesis. Cancer Res. 2020;80:2751-2763 pubmed publisher
Chen G, Xie W, Nah J, Sauvat A, Liu P, Pietrocola F, et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med. 2019;11:e10469 pubmed publisher
Wang H, Huang F, Zhang Z, Wang P, Luo Y, Li H, et al. Feedback Activation of SGK3 and AKT Contributes to Rapamycin Resistance by Reactivating mTORC1/4EBP1 Axis via TSC2 in Breast Cancer. Int J Biol Sci. 2019;15:929-941 pubmed publisher
Ranek M, Kokkonen Simon K, Chen A, Dunkerly Eyring B, Vera M, Oeing C, et al. PKG1-modified TSC2 regulates mTORC1 activity to counter adverse cardiac stress. Nature. 2019;566:264-269 pubmed publisher
Bissler J, Zadjali F, Bridges D, Astrinidis A, Barone S, Yao Y, et al. Tuberous sclerosis complex exhibits a new renal cystogenic mechanism. Physiol Rep. 2019;7:e13983 pubmed publisher
Nnah I, Wang B, Saqcena C, Weber G, Bonder E, Bagley D, et al. TFEB-driven endocytosis coordinates MTORC1 signaling and autophagy. Autophagy. 2019;15:151-164 pubmed publisher
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
Li H, Ren Y, Mao K, Hua F, Yang Y, Wei N, et al. FTO is involved in Alzheimer's disease by targeting TSC1-mTOR-Tau signaling. Biochem Biophys Res Commun. 2018;498:234-239 pubmed publisher
Zou J, Zhang B, Gutmann D, Wong M. Postnatal reduction of tuberous sclerosis complex 1 expression in astrocytes and neurons causes seizures in an age-dependent manner. Epilepsia. 2017;58:2053-2063 pubmed publisher
Zabala Letona A, Arruabarrena Aristorena A, Martin Martin N, Fernandez Ruiz S, Sutherland J, Clasquin M, et al. mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer. Nature. 2017;547:109-113 pubmed publisher
Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-Î²-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
Bakula D, Müller A, Zuleger T, Takacs Z, Franz Wachtel M, Thost A, et al. WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy. Nat Commun. 2017;8:15637 pubmed publisher
Merhi A, Delree P, Marini A. The metabolic waste ammonium regulates mTORC2 and mTORC1 signaling. Sci Rep. 2017;7:44602 pubmed publisher
Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
Jacobs B, McNally R, Kim K, Blanco R, Privett R, You J, et al. Identification of mechanically regulated phosphorylation sites on tuberin (TSC2) that control mechanistic target of rapamycin (mTOR) signaling. J Biol Chem. 2017;292:6987-6997 pubmed publisher
Ercan E, Han J, Di Nardo A, Winden K, Han M, Hoyo L, et al. Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. J Exp Med. 2017;214:681-697 pubmed publisher
. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
Villar V, Nguyen T, Delcroix V, Terés S, Bouchecareilh M, Salin B, et al. mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation. Nat Commun. 2017;8:14124 pubmed publisher
Rahman A, Haugh J. Kinetic Modeling and Analysis of the Akt/Mechanistic Target of Rapamycin Complex 1 (mTORC1) Signaling Axis Reveals Cooperative, Feedforward Regulation. J Biol Chem. 2017;292:2866-2872 pubmed publisher
Yamauchi T, Nishiyama M, Moroishi T, Kawamura A, Nakayama K. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol. 2017;37: pubmed publisher
Hayakawa M, Hayakawa H, Petrova T, Ritprajak P, Sutavani R, Jiménez Andrade G, et al. Loss of Functionally Redundant p38 Isoforms in T Cells Enhances Regulatory T Cell Induction. J Biol Chem. 2017;292:1762-1772 pubmed publisher
Wu L, Zhou B, Oshiro Rapley N, Li M, Paulo J, Webster C, et al. An Ancient, Unified Mechanism for Metformin Growth Inhibition in C. elegans and Cancer. Cell. 2016;167:1705-1718.e13 pubmed publisher
Fettweis G, Di Valentin E, L homme L, Lassence C, Dequiedt F, Fillet M, et al. RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death. Biochim Biophys Acta Mol Cell Res. 2017;1864:113-124 pubmed publisher
Cao J, Tyburczy M, Moss J, Darling T, Widlund H, Kwiatkowski D. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest. 2017;127:349-364 pubmed publisher
Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
Mirkheshti N, Park S, Jiang S, Cropper J, Werner S, Song C, et al. Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer. Oncotarget. 2016;7:62240-62254 pubmed publisher
Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher
Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
Hakim S, Dyson J, Feeney S, Davies E, Sriratana A, Koenig M, et al. Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling. Hum Mol Genet. 2016;25:2295-2313 pubmed
Demetriades C, Plescher M, Teleman A. Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nat Commun. 2016;7:10662 pubmed publisher
Ding M, Bruick R, Yu Y. Secreted IGFBP5 mediates mTORC1-dependent feedback inhibition of IGF-1 signalling. Nat Cell Biol. 2016;18:319-27 pubmed publisher
Llanos S, GarcÃa Pedrero J, Morgado Palacin L, Rodrigo J, Serrano M. Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers. Nat Commun. 2016;7:10438 pubmed publisher
Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
Vural A, Al Khodor S, Cheung G, Shi C, Srinivasan L, McQuiston T, et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol. 2016;196:846-56 pubmed publisher
Yan Y, Ollila S, Wong I, Vallenius T, Palvimo J, Vaahtomeri K, et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 2015;6:8979 pubmed publisher
Ye Z, Al Aidaroos A, Park J, Yuen H, Zhang S, Gupta A, et al. PRL-3 activates mTORC1 in Cancer Progression. Sci Rep. 2015;5:17046 pubmed publisher
Das R, Xu S, Nguyen T, Quan X, Choi S, Kim S, et al. Transforming Growth Factor Î²1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis. J Biol Chem. 2015;290:30830-42 pubmed publisher
Zhang S, Schneider L, Vick B, Grunert M, Jeremias I, Menche D, et al. Anti-leukemic effects of the V-ATPase inhibitor Archazolid A. Oncotarget. 2015;6:43508-28 pubmed publisher
Agarwal S, Bell C, Taylor S, Moran R. p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res. 2016;14:66-77 pubmed publisher
Plescher M, Teleman A, Demetriades C. TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1. Sci Rep. 2015;5:13828 pubmed publisher
Su X, Yu Y, Zhong Y, Giannopoulou E, Hu X, Liu H, et al. Interferon-Î³ regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol. 2015;16:838-849 pubmed publisher
Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, et al. Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest. 2015;125:2707-20 pubmed publisher
Espana Agusti J, Tuveson D, Adams D, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep. 2015;5:11061 pubmed publisher
Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
Shi Y, Tan S, Ng S, Zhou J, Yang N, Koo G, et al. Critical role of CAV1/caveolin-1 in cell stress responses in human breast cancer cells via modulation of lysosomal function and autophagy. Autophagy. 2015;11:769-84 pubmed publisher
RoffÃ© M, Lupinacci F, Soares L, Hajj G, Martins V. Two widely used RSK inhibitors, BI-D1870 and SL0101, alter mTORC1 signaling in a RSK-independent manner. Cell Signal. 2015;27:1630-42 pubmed publisher
Harris White M, Ferbas K, Johnson M, Eslami P, Poteshkina A, Venkova K, et al. A cell-penetrating ester of the neural metabolite lanthionine ketimine stimulates autophagy through the mTORC1 pathway: Evidence for a mechanism of action with pharmacological implications for neurodegenerative pathologies. Neurobiol Dis. 2015;84:60-8 pubmed publisher
Verma R, Marchese A. The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. J Biol Chem. 2015;290:6810-24 pubmed publisher
Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
Peng M, Yin N, Li M. Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Cell. 2014;159:122-133 pubmed publisher
Bartley C, O Keefe R, Bordey A. FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS ONE. 2014;9:e96956 pubmed publisher
Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher
Areta J, Burke L, Ross M, Camera D, West D, Broad E, et al. Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. J Physiol. 2013;591:2319-31 pubmed publisher
Boer K, Troost D, Jansen F, Nellist M, van den Ouweland A, Geurts J, et al. Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex. Neuropathology. 2008;28:577-90 pubmed publisher

articles

contribute

If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.

related gene review

questions and comments