This is a Validated Antibody Database (VAD) review about human TSC1, based on 35 published articles (read how Labome selects the articles), using TSC1 antibody in all methods. It is aimed to help Labome visitors find the most suited TSC1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TSC1 synonym: LAM; TSC

Knockout validation
Invitrogen
domestic goat polyclonal
  • immunocytochemistry knockout validation; mouse; 1:200; fig 1
  • western blot; mouse; 1:200; fig 1
In order to study the control of homoeostasis and survival of myelinating oligodendrocytes by regulation of PERK-eIF2alpha signaling by tuberous sclerosis complex-1, Invitrogen TSC1 antibody (Pierce, PA5-18506) was used in immunocytochemistry knockout validation on mouse samples at 1:200 (fig 1) and in western blot on mouse samples at 1:200 (fig 1). Nat Commun (2016) ncbi
Invitrogen
mouse monoclonal (5C8A12)
  • western blot knockout validation; mouse; fig 1
In order to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation, Invitrogen TSC1 antibody (Invitrogen, 37-0400) was used in western blot knockout validation on mouse samples (fig 1). Cell Cycle (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; loading ...; fig 3f
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot knockout validation on mouse samples (fig 3f). Cell Death Dis (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; 1:200; loading ...; fig 1a
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 6935) was used in western blot knockout validation on mouse samples at 1:200 (fig 1a). Front Immunol (2021) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 1a
In order to determine the effect of constitutive activation of mammalian target of rapamycin complex 1 on premature senescence activation in fibroblasts, Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot knockout validation on mouse samples (fig 1a). EMBO J (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; loading ...; fig 2b
In order to show that pituitary tumor transforming gene 1 expression is upregulated in an mammalian/mechanistic target of rapamycin complex 1-dependent manner, Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot knockout validation on mouse samples (fig 2b). Oncogene (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig s7c
  • western blot; human; 1:1000; loading ...; fig s9a
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig s7a
Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in immunocytochemistry on human samples at 1:200 (fig s7c), in western blot on human samples at 1:1000 (fig s9a) and in immunocytochemistry knockout validation on mouse samples at 1:200 (fig s7a). Nat Commun (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; loading ...; fig 5c
  • western blot; mouse; 1:1000; loading ...; fig 5a
Invitrogen TSC1 antibody (ThermoFisher, PA5-20131) was used in immunohistochemistry on mouse samples at 1:500 (fig 5c) and in western blot on mouse samples at 1:1000 (fig 5a). Nature (2019) ncbi
domestic goat polyclonal
  • immunocytochemistry knockout validation; mouse; 1:200; fig 1
  • western blot; mouse; 1:200; fig 1
In order to study the control of homoeostasis and survival of myelinating oligodendrocytes by regulation of PERK-eIF2alpha signaling by tuberous sclerosis complex-1, Invitrogen TSC1 antibody (Pierce, PA5-18506) was used in immunocytochemistry knockout validation on mouse samples at 1:200 (fig 1) and in western blot on mouse samples at 1:200 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (5C8A12)
  • western blot knockout validation; mouse; fig 1
In order to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation, Invitrogen TSC1 antibody (Invitrogen, 37-0400) was used in western blot knockout validation on mouse samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (5C8A12)
  • western blot; human; fig 5
In order to investigate if and how mTOR regulates YAP, Invitrogen TSC1 antibody (Invitrogen, 37-0400) was used in western blot on human samples (fig 5). J Exp Med (2014) ncbi
mouse monoclonal (5C8A12)
  • western blot; human; fig 1
In order to study signaling pathways underlying HBx-induced liver tumorigenesis, Invitrogen TSC1 antibody (ZYMED, 37-0400) was used in western blot on human samples (fig 1). PLoS ONE (2012) ncbi
mouse monoclonal (5C8A12)
  • western blot; human; fig 3
In order to elucidate how leucine is sensed and transmitted to mTOR, Invitrogen TSC1 antibody (Zymed, 37-0400) was used in western blot on human samples (fig 3). Genes Cells (2010) ncbi
mouse monoclonal (5C8A12)
  • western blot; human; fig 4d
In order to suggest ARD1 inhibits the mTOR pathway, Invitrogen TSC1 antibody (Zymed, 37-0400) was used in western blot on human samples (fig 4d). Sci Signal (2010) ncbi
mouse monoclonal (5C8A12)
  • immunocytochemistry; human; 1:100; fig 1
  • western blot; human; fig 2
In order to characterize the ciliary disruption in tuberous sclerosis complex syndrome, Invitrogen TSC1 antibody (Zymed, 37-0400) was used in immunocytochemistry on human samples at 1:100 (fig 1) and in western blot on human samples (fig 2). Hum Mol Genet (2009) ncbi
mouse monoclonal (5C8A12)
  • immunohistochemistry - paraffin section; human; 1:70; tbl 1
In order to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene, Invitrogen TSC1 antibody (Zymed, 5C8A12) was used in immunohistochemistry - paraffin section on human samples at 1:70 (tbl 1). Neuropathology (2008) ncbi
mouse monoclonal (5C8A12)
  • western blot; mouse; fig 4a
In order to investigate inflammation-mediated tumor angiogenesis, Invitrogen TSC1 antibody (Zymed, 37-0400) was used in western blot on mouse samples (fig 4a). Cell (2007) ncbi
R&D Systems
mouse monoclonal (488915)
  • immunoprecipitation; human
In order to identify molecular targets that suppress tumorigenesis in triple negative breast cancers, R&D Systems TSC1 antibody (R&D Systems, MAB4379) was used in immunoprecipitation on human samples . J Clin Invest (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; 1:1000; loading ...; fig 1c, s1a, 4d
Cell Signaling Technology TSC1 antibody (CST, 6935) was used in western blot on mouse samples at 1:1000 (fig 1c, s1a, 4d). Cell Rep (2022) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; loading ...; fig 2a
Cell Signaling Technology TSC1 antibody (Cell signaling, 6935) was used in western blot on mouse samples (fig 2a). Mol Metab (2022) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; loading ...; fig 3f
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot knockout validation on mouse samples (fig 3f). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2f
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 4906) was used in western blot on human samples (fig 2f). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; 1:1000; loading ...; fig s12
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on mouse samples at 1:1000 (fig s12). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; 1:200; loading ...; fig 1a
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 6935) was used in western blot knockout validation on mouse samples at 1:200 (fig 1a). Front Immunol (2021) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology TSC1 antibody (Cell Signaling Technology, 6935) was used in western blot on human samples at 1:1000. Nat Commun (2021) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; 1:1000; loading ...; fig 2b
Cell Signaling Technology TSC1 antibody (Cell signaling, 6935) was used in western blot on mouse samples at 1:1000 (fig 2b). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3a
Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot on human samples (fig 3a). Sci Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig ev3e
Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot on human samples (fig ev3e). EMBO J (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; loading ...; fig 3d
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on mouse samples (fig 3d). Biochem Biophys Res Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1a
In order to research the role of tuberous sclerosis complex 1 in epilepsy and its mechanism, Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot on mouse samples (fig 1a). Epilepsia (2017) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; human; 1:1000; loading ...; fig 5a
In order to research the roles of WIPI3 and WIPI4 in autophagy, Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on human samples at 1:1000 (fig 5a). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology TSC1 antibody (CST, 4906) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 1a
In order to determine the effect of constitutive activation of mammalian target of rapamycin complex 1 on premature senescence activation in fibroblasts, Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot knockout validation on mouse samples (fig 1a). EMBO J (2017) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; mouse; loading ...; fig 1d
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on mouse samples (fig 1d). Hum Mol Genet (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1a
Cell Signaling Technology TSC1 antibody (cell signalling, 4906) was used in western blot on human samples (fig 1a). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot knockout validation; mouse; loading ...; fig 2b
In order to show that pituitary tumor transforming gene 1 expression is upregulated in an mammalian/mechanistic target of rapamycin complex 1-dependent manner, Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot knockout validation on mouse samples (fig 2b). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 6
In order to assess the inhibition of mammalian target of rapamycin complex 1 in mice due to fibroblast growth factor 21 improving hepatic insulin sensitivity, Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in western blot on mouse samples (fig 6). Hepatology (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig s7c
  • western blot; human; 1:1000; loading ...; fig s9a
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig s7a
Cell Signaling Technology TSC1 antibody (Cell Signaling, 4906) was used in immunocytochemistry on human samples at 1:200 (fig s7c), in western blot on human samples at 1:1000 (fig s9a) and in immunocytochemistry knockout validation on mouse samples at 1:200 (fig s7a). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 2e
In order to survey mTORC1 activity reguled by control of TSC2-Rheb signaling by arginine, Cell Signaling Technology TSC1 antibody (Cell Signaling Technologies, 4906) was used in western blot on human samples at 1:1000 (fig 2e). elife (2016) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; human; fig 1
Cell Signaling Technology TSC1 antibody (Cell Signaling Tech, 6935s) was used in western blot on human samples (fig 1). Int J Mol Med (2015) ncbi
domestic rabbit monoclonal (D43E2)
  • western blot; rat
Cell Signaling Technology TSC1 antibody (Cell Signaling, 6935) was used in western blot on rat samples . Mol Cell Biol (2015) ncbi
Articles Reviewed
  1. Schr xf6 tter S, Yuskaitis C, MacArthur M, Mitchell S, Hosios A, Osipovich M, et al. The non-essential TSC complex component TBC1D7 restricts tissue mTORC1 signaling and brain and neuron growth. Cell Rep. 2022;39:110824 pubmed publisher
  2. Han H, Kim S, Kim Y, Jang S, Kwon Y, Choi D, et al. A novel role of CRTC2 in promoting nonalcoholic fatty liver disease. Mol Metab. 2022;55:101402 pubmed publisher
  3. Chen X, Miao M, Zhou M, Chen J, Li D, Zhang L, et al. Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway. Cell Death Dis. 2021;12:761 pubmed publisher
  4. Alesi N, Akl E, Khabibullin D, Liu H, Nidhiry A, Garner E, et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021;12:4245 pubmed publisher
  5. Zhang T, He M, Zhao L, Qin S, Zhu Z, Du X, et al. HDAC6 regulates primordial follicle activation through mTOR signaling pathway. Cell Death Dis. 2021;12:559 pubmed publisher
  6. Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  7. Zhang Y, Swanda R, Nie L, Liu X, Wang C, Lee H, et al. mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation. Nat Commun. 2021;12:1589 pubmed publisher
  8. Jewell J, Fu V, Hong A, Yu F, Meng D, Melick C, et al. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. elife. 2019;8: pubmed publisher
  9. Mukhopadhyay U, Chanda S, Patra U, Mukherjee A, Rana S, Mukherjee A, et al. Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy. Sci Rep. 2019;9:1318 pubmed publisher
  10. Poulopoulos A, Murphy A, Ozkan A, Davis P, Hatch J, Kirchner R, et al. Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex. Nature. 2019;565:356-360 pubmed publisher
  11. Hartleben G, Muller C, Kramer A, Schimmel H, Zidek L, Dornblut C, et al. Tuberous sclerosis complex is required for tumor maintenance in MYC-driven Burkitt's lymphoma. EMBO J. 2018;37: pubmed publisher
  12. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  13. Li H, Ren Y, Mao K, Hua F, Yang Y, Wei N, et al. FTO is involved in Alzheimer's disease by targeting TSC1-mTOR-Tau signaling. Biochem Biophys Res Commun. 2018;498:234-239 pubmed publisher
  14. Zou J, Zhang B, Gutmann D, Wong M. Postnatal reduction of tuberous sclerosis complex 1 expression in astrocytes and neurons causes seizures in an age-dependent manner. Epilepsia. 2017;58:2053-2063 pubmed publisher
  15. Bakula D, Müller A, Zuleger T, Takacs Z, Franz Wachtel M, Thost A, et al. WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy. Nat Commun. 2017;8:15637 pubmed publisher
  16. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  17. Barilari M, Bonfils G, Treins C, Koka V, De Villeneuve D, Fabrega S, et al. ZRF1 is a novel S6 kinase substrate that drives the senescence programme. EMBO J. 2017;36:736-750 pubmed publisher
  18. Jin F, Jiang K, Ji S, Wang L, Ni Z, Huang F, et al. Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex. Hum Mol Genet. 2017;26:407-419 pubmed publisher
  19. Cao J, Tyburczy M, Moss J, Darling T, Widlund H, Kwiatkowski D. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest. 2017;127:349-364 pubmed publisher
  20. Chen R, Duan J, Li L, Ma Q, Sun Q, Ma J, et al. mTOR promotes pituitary tumor development through activation of PTTG1. Oncogene. 2017;36:979-988 pubmed publisher
  21. Jiang M, Liu L, He X, Wang H, Lin W, Wang H, et al. Regulation of PERK-eIF2? signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes. Nat Commun. 2016;7:12185 pubmed publisher
  22. Gong Q, Hu Z, Zhang F, Cui A, Chen X, Jiang H, et al. Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice. Hepatology. 2016;64:425-38 pubmed publisher
  23. Demetriades C, Plescher M, Teleman A. Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nat Commun. 2016;7:10662 pubmed publisher
  24. Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
  25. Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, et al. Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest. 2015;125:2707-20 pubmed publisher
  26. Li L, Qi L, Liang Z, Song W, Liu Y, Wang Y, et al. Transforming growth factor-β1 induces EMT by the transactivation of epidermal growth factor signaling through HA/CD44 in lung and breast cancer cells. Int J Mol Med. 2015;36:113-22 pubmed publisher
  27. Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher
  28. Ma X, Liu H, Murphy J, Foyil S, Godar R, Abuirqeba H, et al. Regulation of the transcription factor EB-PGC1α axis by beclin-1 controls mitochondrial quality and cardiomyocyte death under stress. Mol Cell Biol. 2015;35:956-76 pubmed publisher
  29. Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
  30. Yen C, Lin Y, Yen C, Tsai H, Tsai T, Chang K, et al. Hepatitis B virus X protein upregulates mTOR signaling through IKKβ to increase cell proliferation and VEGF production in hepatocellular carcinoma. PLoS ONE. 2012;7:e41931 pubmed publisher
  31. Kume K, Iizumi Y, Shimada M, Ito Y, Kishi T, Yamaguchi Y, et al. Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway. Genes Cells. 2010;15:339-49 pubmed publisher
  32. Kuo H, Lee D, Chen C, Liu M, Chou C, Lee H, et al. ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. Sci Signal. 2010;3:ra9 pubmed publisher
  33. Hartman T, Liu D, Zilfou J, Robb V, Morrison T, Watnick T, et al. The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway. Hum Mol Genet. 2009;18:151-63 pubmed publisher
  34. Boer K, Troost D, Jansen F, Nellist M, van den Ouweland A, Geurts J, et al. Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex. Neuropathology. 2008;28:577-90 pubmed publisher
  35. Lee D, Kuo H, Chen C, Hsu J, Chou C, Wei Y, et al. IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. Cell. 2007;130:440-55 pubmed