This is a Validated Antibody Database (VAD) review about human TRPV4, based on 13 published articles (read how Labome selects the articles), using TRPV4 antibody in all methods. It is aimed to help Labome visitors find the most suited TRPV4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TRPV4 synonym: BCYM3; CMT2C; HMSN2C; OTRPC4; SMAL; SPSMA; SSQTL1; TRP12; VRL2; VROAC; transient receptor potential cation channel subfamily V member 4; OSM9-like transient receptor potential channel 4; osm-9-like TRP channel 4; osmosensitive transient receptor potential channel 4; transient receptor potential protein 12; vanilloid receptor-like channel 2; vanilloid receptor-related osmotically activated channel

Abcam
rabbit polyclonal
  • western blot; human; fig 4
In order to study osteochondrodysplasia in Scottish fold cats by an underlying dominant TRPV4 variant, Abcam TRPV4 antibody (Abcam, ab39260) was used in western blot on human samples (fig 4). Osteoarthritis Cartilage (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200
  • immunocytochemistry; mouse; 1:200
  • western blot; mouse; 1:500
  • immunohistochemistry - frozen section; pig; 1:200
  • western blot; pig; 1:500
In order to examine the regulatory mechanism of blood-cerebrospinal fluid barrier, Abcam TRPV4 antibody (Abcam, ab39260) was used in immunohistochemistry - frozen section on mouse samples at 1:200, in immunocytochemistry on mouse samples at 1:200, in western blot on mouse samples at 1:500, in immunohistochemistry - frozen section on pig samples at 1:200 and in western blot on pig samples at 1:500. FASEB J (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:500
  • western blot; human; 1:500
Abcam TRPV4 antibody (Abcam, ab39260) was used in immunohistochemistry - frozen section on human samples at 1:500 and in western blot on human samples at 1:500. Biomed Res Int (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:1000
In order to determine the expression and role of aquaporins in spinal cord, Abcam TRPV4 antibody (Abcam, AB39260) was used in immunohistochemistry - paraffin section on rat samples at 1:1000. J Histochem Cytochem (2014) ncbi
rabbit polyclonal
  • western blot; rat; 5 ug/ml
Abcam TRPV4 antibody (Abcam, Ab39260) was used in western blot on rat samples at 5 ug/ml. Neuroscience (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000
In order to study the elevated urinary bladder and lumbosacral dorsal root ganglion expression of TRPV4 in transgenic mice overexpressing NGF in the urothelium, Abcam TRPV4 antibody (Abcam, Ab39260) was used in immunohistochemistry - frozen section on mouse samples at 1:1000. J Mol Neurosci (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse
Abcam TRPV4 antibody (Abcam, ab39260) was used in immunohistochemistry on mouse samples . J Clin Invest (2013) ncbi
Alomone Labs
rabbit polyclonal
  • immunocytochemistry; rat; 1:1500; fig 6
Alomone Labs TRPV4 antibody (Alomone Labs, ACC-034) was used in immunocytochemistry on rat samples at 1:1500 (fig 6). J Cell Biochem (2018) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 1c
Alomone Labs TRPV4 antibody (Alomone Labs, ACC-034) was used in immunohistochemistry on mouse samples (fig 1c). Sci Rep (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig 5
  • immunocytochemistry; mouse; 1:100; fig 3
  • western blot; mouse; 1:1000; fig 1
In order to utilize the mechanosensitive TRPV4 channel in the collecting duct system of kidney that is coupled to expression of a diverse array of Ca2+-activated K+ channels (SK1/3, IK1, BK), Alomone Labs TRPV4 antibody (Alomone, ACC-034) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5), in immunocytochemistry on mouse samples at 1:100 (fig 3) and in western blot on mouse samples at 1:1000 (fig 1). PLoS ONE (2016) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 1e
  • immunocytochemistry; human; 1:1000; fig 1c
  • immunohistochemistry; human; 1:1000; loading ...; fig 1f
  • western blot; human; fig 1b
In order to suggest that TRPV4 channels are involved in the mechanosensitive, calcium-signaling machinery within the TM, LifeSpan Biosciences TRPV4 antibody (Lifespan, C94498) was used in immunohistochemistry on mouse samples at 1:1000 (fig 1e), in immunocytochemistry on human samples at 1:1000 (fig 1c), in immunohistochemistry on human samples at 1:1000 (fig 1f) and in western blot on human samples (fig 1b). Sci Rep (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; rat; 1:500
In order to investigate the role of TRPV4 in stress-induced bladder dysfunction using an a rat model, Invitrogen TRPV4 antibody (Fisher Scientific, OSR00136W) was used in western blot on rat samples at 1:500. Am J Physiol Regul Integr Comp Physiol (2014) ncbi
MilliporeSigma
rabbit polyclonal
  • immunohistochemistry; rat; 1:100
MilliporeSigma TRPV4 antibody (Sigma, HPA007150) was used in immunohistochemistry on rat samples at 1:100. BJU Int (2015) ncbi
Articles Reviewed
  1. Pizzoni A, López González M, Di Giusto G, Rivarola V, Capurro C, Ford P. AQP2 can modulate the pattern of Ca2+ transients induced by store-operated Ca2+ entry under TRPV4 activation. J Cell Biochem. 2018;119:4120-4133 pubmed publisher
  2. Dong Q, Li J, Wu Q, Zhao N, Qian C, Ding D, et al. Blockage of transient receptor potential vanilloid 4 alleviates myocardial ischemia/reperfusion injury in mice. Sci Rep. 2017;7:42678 pubmed publisher
  3. Ryskamp D, Frye A, Phuong T, Yarishkin O, Jo A, Xu Y, et al. TRPV4 regulates calcium homeostasis, cytoskeletal remodeling, conventional outflow and intraocular pressure in the mammalian eye. Sci Rep. 2016;6:30583 pubmed publisher
  4. Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
  5. Gandolfi B, Alamri S, Darby W, Adhikari B, Lattimer J, Malik R, et al. A dominant TRPV4 variant underlies osteochondrodysplasia in Scottish fold cats. Osteoarthritis Cartilage. 2016;24:1441-50 pubmed publisher
  6. Narita K, Sasamoto S, Koizumi S, Okazaki S, Nakamura H, Inoue T, et al. TRPV4 regulates the integrity of the blood-cerebrospinal fluid barrier and modulates transepithelial protein transport. FASEB J. 2015;29:2247-59 pubmed publisher
  7. Charrua A, Cruz C, Jansen D, Rozenberg B, Heesakkers J, Cruz F. Co-administration of transient receptor potential vanilloid 4 (TRPV4) and TRPV1 antagonists potentiate the effect of each drug in a rat model of cystitis. BJU Int. 2015;115:452-60 pubmed publisher
  8. Merrill L, Vizzard M. Intravesical TRPV4 blockade reduces repeated variate stress-induced bladder dysfunction by increasing bladder capacity and decreasing voiding frequency in male rats. Am J Physiol Regul Integr Comp Physiol. 2014;307:R471-80 pubmed publisher
  9. Sterle I, Zupancic D, Romih R. Correlation between urothelial differentiation and sensory proteins P2X3, P2X5, TRPV1, and TRPV4 in normal urothelium and papillary carcinoma of human bladder. Biomed Res Int. 2014;2014:805236 pubmed publisher
  10. Oklinski M, Lim J, Choi H, Oklinska P, Skowronski M, Kwon T. Immunolocalization of Water Channel Proteins AQP1 and AQP4 in Rat Spinal Cord. J Histochem Cytochem. 2014;62:598-611 pubmed publisher
  11. Shenton F, Pyner S. Expression of transient receptor potential channels TRPC1 and TRPV4 in venoatrial endocardium of the rat heart. Neuroscience. 2014;267:195-204 pubmed publisher
  12. Girard B, Merrill L, Malley S, Vizzard M. Increased TRPV4 expression in urinary bladder and lumbosacral dorsal root ganglia in mice with chronic overexpression of NGF in urothelium. J Mol Neurosci. 2013;51:602-14 pubmed publisher
  13. Mittag J, Lyons D, Sällström J, Vujovic M, Dudazy Gralla S, Warner A, et al. Thyroid hormone is required for hypothalamic neurons regulating cardiovascular functions. J Clin Invest. 2013;123:509-16 pubmed publisher