This is a Validated Antibody Database (VAD) review about human TRIM28, based on 31 published articles (read how Labome selects the articles), using TRIM28 antibody in all methods. It is aimed to help Labome visitors find the most suited TRIM28 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TRIM28 synonym: KAP1; PPP1R157; RNF96; TF1B; TIF1B

Knockout validation
Abcam
mouse monoclonal (20C1)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 3c
Abcam TRIM28 antibody (Abcam, ab22553) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 3c). Stem Cell Reports (2020) ncbi
Abcam
domestic rabbit polyclonal
  • immunoprecipitation; human; 1:1000; loading ...; fig 2c
  • immunocytochemistry; human; loading ...; fig 2e
  • western blot; human; loading ...; fig 2g
Abcam TRIM28 antibody (Abcam, ab10484) was used in immunoprecipitation on human samples at 1:1000 (fig 2c), in immunocytochemistry on human samples (fig 2e) and in western blot on human samples (fig 2g). NAR Cancer (2021) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6c
  • western blot; human; 1:1000; loading ...; fig 4c
Abcam TRIM28 antibody (Abcam, ab10484) was used in immunoprecipitation on human samples (fig 6c) and in western blot on human samples at 1:1000 (fig 4c). Mol Med Rep (2021) ncbi
mouse monoclonal (20C1)
  • western blot; human; loading ...; fig 2c, 2h
Abcam TRIM28 antibody (Abcam, Ab22553) was used in western blot on human samples (fig 2c, 2h). Nucleic Acids Res (2020) ncbi
mouse monoclonal (20C1)
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 3c
Abcam TRIM28 antibody (Abcam, ab22553) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 3c). Stem Cell Reports (2020) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2a
Abcam TRIM28 antibody (Abcam, 20C1) was used in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 2a). PLoS Pathog (2019) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; mouse; 1:1000; loading ...; fig 4b
Abcam TRIM28 antibody (Abcam, ab22553) was used in immunoprecipitation on mouse samples at 1:1000 (fig 4b). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4f
Abcam TRIM28 antibody (Abcam, ab10484) was used in western blot on mouse samples (fig 4f). Sci Adv (2019) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3a
Abcam TRIM28 antibody (Abcam, ab22553) was used in immunoprecipitation on human samples (fig 3d) and in western blot on human samples (fig 3a). Proc Natl Acad Sci U S A (2019) ncbi
mouse monoclonal (20C1)
  • western blot; human; loading ...; fig 2d
Abcam TRIM28 antibody (Abcam, ab22553) was used in western blot on human samples (fig 2d). Sci Signal (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7b
In order to study the contribution of PAXX to non-homologous end-joining, Abcam TRIM28 antibody (Abcam, ab10484) was used in western blot on mouse samples at 1:1000 (fig 7b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
  • western blot; mouse; loading ...; fig 4c
In order to identify PEG3 as a KRAB-containing zinc finger protein that interacts with KAP1, Abcam TRIM28 antibody (Abcam, ab10484) was used in chromatin immunoprecipitation on mouse samples (fig 5a) and in western blot on mouse samples (fig 4c). PLoS ONE (2016) ncbi
mouse monoclonal (20C1)
  • immunocytochemistry; mouse; 1:500
  • western blot; mouse; 1:1000
In order to characterize TRIM28 as an epigenetic barrier to generate reprogramming of pluripotent stem cells, Abcam TRIM28 antibody (Abcam, ab22553) was used in immunocytochemistry on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. Stem Cells (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 6
Abcam TRIM28 antibody (Abcam, ab10483) was used in western blot on human samples (fig 6). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s3
Abcam TRIM28 antibody (Abcam, ab10484) was used in western blot on mouse samples (fig s3). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 4
In order to elucidate the complex role of the BAHD1 c hromatin-repressive complex in regulation of steroid metabolism and placental development, Abcam TRIM28 antibody (Abcam, ab10483) was used in western blot on mouse samples (fig 4). PLoS Genet (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig s1
Abcam TRIM28 antibody (Abcam, ab10483) was used in western blot on mouse samples at 1:1000 (fig s1). elife (2016) ncbi
mouse monoclonal (20C1)
  • EMSA; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 5a
Abcam TRIM28 antibody (Abcam, ab22553) was used in EMSA on human samples (fig 4a) and in western blot on human samples (fig 5a). PLoS ONE (2015) ncbi
mouse monoclonal (20C1)
  • western blot; mouse
Abcam TRIM28 antibody (Abcam, ab22553) was used in western blot on mouse samples . DNA Res (2015) ncbi
mouse monoclonal (20C1)
  • immunoprecipitation; human; fig s3e
  • immunocytochemistry; human; 1:500; fig s4b
  • western blot; human; fig s3c
Abcam TRIM28 antibody (Abcam, ab22553) was used in immunoprecipitation on human samples (fig s3e), in immunocytochemistry on human samples at 1:500 (fig s4b) and in western blot on human samples (fig s3c). Cell Rep (2015) ncbi
mouse monoclonal (20C1)
  • chromatin immunoprecipitation; mouse
Abcam TRIM28 antibody (Abcam, ab22553) was used in chromatin immunoprecipitation on mouse samples . J Virol (2014) ncbi
mouse monoclonal (20C1)
  • western blot; human
Abcam TRIM28 antibody (Abcam, ab22553) was used in western blot on human samples . PLoS ONE (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • proximity ligation assay; human; loading ...; fig 4b
Santa Cruz Biotechnology TRIM28 antibody (Santa Cruz, sc-515790) was used in proximity ligation assay on human samples (fig 4b). Sci Rep (2020) ncbi
Invitrogen
domestic goat polyclonal
  • western blot; human; loading ...; fig s5l
In order to demonstrate that iPS-derived cardiomyocytes with a heterozygous GATA4-G296S missense mutation show impaired contractility, calcium handling, and metabolic activity, Invitrogen TRIM28 antibody (Thermo Fisher, PA1-9059) was used in western blot on human samples (fig s5l). Cell (2016) ncbi
Enzo Life Sciences
mouse monoclonal (20A1)
  • western blot; human
In order to identify targets of SUMO2/3 during mitosis, Enzo Life Sciences TRIM28 antibody (Enzo Life Sciences, ADI-KAM-TF200) was used in western blot on human samples . Proteomics (2015) ncbi
Novus Biologicals
domestic rabbit polyclonal (2D8)
Novus Biologicals TRIM28 antibody (Novus Biologicals, NB500-158) was used . EMBO J (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C42G12)
  • western blot; human; 1:1000; loading ...; fig s4a
Cell Signaling Technology TRIM28 antibody (Cell Signaling Technology, 4124) was used in western blot on human samples at 1:1000 (fig s4a). Front Oncol (2020) ncbi
domestic rabbit monoclonal (C42G12)
  • immunoprecipitation; human; fig 3h
Cell Signaling Technology TRIM28 antibody (Cell Signaling technology, 4124S) was used in immunoprecipitation on human samples (fig 3h). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology TRIM28 antibody (Cell Signaling, 4123) was used in western blot on human samples (fig 1a). Mol Cell (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology TRIM28 antibody (Cell Signaling, 4127) was used in western blot on mouse samples (fig 5a). Mol Cell Biol (2019) ncbi
domestic rabbit monoclonal (C42G12)
  • immunocytochemistry; mouse; 1:1000; fig s6
  • western blot; mouse; fig s3
Cell Signaling Technology TRIM28 antibody (Cell Signaling, 4124) was used in immunocytochemistry on mouse samples at 1:1000 (fig s6) and in western blot on mouse samples (fig s3). Nat Struct Mol Biol (2015) ncbi
BD Biosciences
mouse monoclonal (23/KRIP1)
  • western blot; human; fig 2i
BD Biosciences TRIM28 antibody (BD Biosciences, 610680) was used in western blot on human samples (fig 2i). J Biol Chem (2015) ncbi
Articles Reviewed
  1. Zhu X, Wang X, Yan W, Yang H, Xiang Y, Lv F, et al. Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response. NAR Cancer. 2021;3:zcab010 pubmed publisher
  2. Zhang X, Zhao H, Wang Y, Di L, Liu X, Qian F, et al. Zenglv Fumai Granule protects cardiomyocytes against hypoxia/reoxygenation-induced apoptosis via inhibiting TRIM28 expression. Mol Med Rep. 2021;23: pubmed publisher
  3. Fang M, Zhang M, Wang Y, Wei F, Wu J, Mou X, et al. Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Front Oncol. 2020;10:601055 pubmed publisher
  4. Oo J, Irmer B, Günther S, Warwick T, Palfi K, Izquierdo Ponce J, et al. ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting. Sci Rep. 2020;10:19079 pubmed publisher
  5. Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, et al. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Res. 2020;48:6001-6018 pubmed publisher
  6. Tan J, Wollmann H, van Pelt A, Kaldis P, Messerschmidt D. Infertility-Causing Haploinsufficiency Reveals TRIM28/KAP1 Requirement in Spermatogonia. Stem Cell Reports. 2020;14:818-827 pubmed publisher
  7. Ding L, Zhu Q, Zhou F, Tan H, Xu W, Pan C, et al. Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma. PLoS Pathog. 2019;15:e1008174 pubmed publisher
  8. Peter C, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun. 2019;10:4112 pubmed publisher
  9. Colomer C, Margalef P, Villanueva A, Vert A, Pecharroman I, Sole L, et al. IKKα Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. Mol Cell. 2019;75:669-682.e5 pubmed publisher
  10. Chen W, Schwalie P, Pankevich E, Gubelmann C, Raghav S, Dainese R, et al. ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer. Nat Commun. 2019;10:1809 pubmed publisher
  11. Ao Y, Zhang J, Liu Z, Qian M, Li Y, Wu Z, et al. Lamin A buffers CK2 kinase activity to modulate aging in a progeria mouse model. Sci Adv. 2019;5:eaav5078 pubmed publisher
  12. So C, Ramachandran S, Martin A. E3 Ubiquitin Ligases RNF20 and RNF40 Are Required for Double-Stranded Break (DSB) Repair: Evidence for Monoubiquitination of Histone H2B Lysine 120 as a Novel Axis of DSB Signaling and Repair. Mol Cell Biol. 2019;39: pubmed publisher
  13. Taura M, Song E, Ho Y, Iwasaki A. Apobec3A maintains HIV-1 latency through recruitment of epigenetic silencing machinery to the long terminal repeat. Proc Natl Acad Sci U S A. 2019;116:2282-2289 pubmed publisher
  14. Lee J, Mand M, Kao C, Zhou Y, Ryu S, Richards A, et al. ATM directs DNA damage responses and proteostasis via genetically separable pathways. Sci Signal. 2018;11: pubmed publisher
  15. Liu X, Shao Z, Jiang W, Lee B, Zha S. PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice. Nat Commun. 2017;8:13816 pubmed publisher
  16. Ang Y, Rivas R, Ribeiro A, Srivas R, Rivera J, Stone N, et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell. 2016;167:1734-1749.e22 pubmed publisher
  17. He H, Ye A, Kim H, Kim J. PEG3 Interacts with KAP1 through KRAB-A. PLoS ONE. 2016;11:e0167541 pubmed publisher
  18. Miles D, de Vries N, Gisler S, Lieftink C, Akhtar W, Gogola E, et al. TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming. Stem Cells. 2017;35:147-157 pubmed publisher
  19. Chiang T, le Sage C, Larrieu D, Demir M, Jackson S. CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016;6:24356 pubmed publisher
  20. Li T, Liu X, Jiang L, MANFREDI J, Zha S, Gu W. Loss of p53-mediated cell-cycle arrest, senescence and apoptosis promotes genomic instability and premature aging. Oncotarget. 2016;7:11838-49 pubmed publisher
  21. Lakisic G, Lebreton A, Pourpre R, Wendling O, Libertini E, Radford E, et al. Role of the BAHD1 Chromatin-Repressive Complex in Placental Development and Regulation of Steroid Metabolism. PLoS Genet. 2016;12:e1005898 pubmed publisher
  22. Walter M, Teissandier A, Pérez Palacios R, Bourc his D. An epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells. elife. 2016;5: pubmed publisher
  23. Guo Y, Feng W, Sy S, Huen M. ATM-dependent Phosphorylation of the Fanconi Anemia Protein PALB2 Promotes the DNA Damage Response. J Biol Chem. 2015;290:27545-56 pubmed publisher
  24. Ichida Y, Utsunomiya Y, Yasuda T, Nakabayashi K, Sato T, Onodera M. Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing. PLoS ONE. 2015;10:e0139274 pubmed publisher
  25. Akiyama T, Xin L, Oda M, Sharov A, Amano M, Piao Y, et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res. 2015;22:307-18 pubmed publisher
  26. Hartlerode A, Morgan M, Wu Y, Buis J, Ferguson D. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat Struct Mol Biol. 2015;22:736-43 pubmed publisher
  27. Xu Y, Zhang H, Nguyen V, Angelopoulos N, Nunes J, Reid A, et al. LMTK3 Represses Tumor Suppressor-like Genes through Chromatin Remodeling in Breast Cancer. Cell Rep. 2015;12:837-49 pubmed publisher
  28. Batenburg N, Thompson E, Hendrickson E, Zhu X. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015;34:1399-416 pubmed publisher
  29. Cubeñas Potts C, Srikumar T, Lee C, Osula O, Subramonian D, Zhang X, et al. Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes. Proteomics. 2015;15:763-72 pubmed publisher
  30. Kao T, Liao H, Wolf D, Tai K, Chuang C, Lee H, et al. Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells. J Virol. 2014;88:10680-95 pubmed publisher
  31. Lee J, Guo Z, Myler L, Zheng S, Paull T. Direct activation of ATM by resveratrol under oxidizing conditions. PLoS ONE. 2014;9:e97969 pubmed publisher