This is a Validated Antibody Database (VAD) review about human TRIM25, based on 9 published articles (read how Labome selects the articles), using TRIM25 antibody in all methods. It is aimed to help Labome visitors find the most suited TRIM25 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TRIM25 synonym: EFP; RNF147; Z147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; estrogen-responsive finger protein; tripartite motif protein TRIM25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147

Knockout validation
BD Biosciences
mouse monoclonal (2/EFP)
  • western blot knockout validation; human; loading ...; fig 5b
BD Biosciences TRIM25 antibody (BD Biosciences, 2/EFP) was used in western blot knockout validation on human samples (fig 5b). J Virol (2018) ncbi
BD Biosciences
mouse monoclonal (2/EFP)
  • western blot knockout validation; human; 1:5000; loading ...; fig s2a
In order to investigate the antiviral mechanism of host factor and interferon-stimulated gene product, zinc-finger antiviral protein, BD Biosciences TRIM25 antibody (BD Biosciences, 610570) was used in western blot knockout validation on human samples at 1:5000 (fig s2a). PLoS Pathog (2017) ncbi
Abcam
rabbit monoclonal (EPR7315)
  • other; human; loading ...; fig 4c
Abcam TRIM25 antibody (Abcam, ab167154) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
rabbit monoclonal (EPR7315)
  • western blot; mouse; 1:1000; loading ...; fig 4k
Abcam TRIM25 antibody (Abcam, ab167154) was used in western blot on mouse samples at 1:1000 (fig 4k). Dis Model Mech (2017) ncbi
rabbit monoclonal (EPR7315)
  • proximity ligation assay; human; loading ...; fig 3c
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 1a
Abcam TRIM25 antibody (Abcam, ab167154) was used in proximity ligation assay on human samples (fig 3c), in immunocytochemistry on human samples (fig 1e) and in western blot on human samples (fig 1a). J Virol (2017) ncbi
rabbit monoclonal (EPR7315)
  • western blot; human; fig 3
Abcam TRIM25 antibody (Abcam, ab167154) was used in western blot on human samples (fig 3). elife (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 7d
  • western blot; mouse; 1:1000; loading ...; fig 6a
In order to examine the contribution of USP15-TRIM25 to neuroinflammation, Proteintech Group TRIM25 antibody (Proteintech, 12573-1-AP) was used in western blot on human samples at 1:1000 (fig 7d) and in western blot on mouse samples at 1:1000 (fig 6a). Nat Immunol (2017) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, Bethyl TRIM25 antibody (Bethyl, A301-856A) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
BD Biosciences
mouse monoclonal (2/EFP)
  • western blot knockout validation; human; loading ...; fig 5b
BD Biosciences TRIM25 antibody (BD Biosciences, 2/EFP) was used in western blot knockout validation on human samples (fig 5b). J Virol (2018) ncbi
mouse monoclonal (2/EFP)
BD Biosciences TRIM25 antibody (BD Biosciences, 610570) was used . Cell Host Microbe (2017) ncbi
mouse monoclonal (2/EFP)
  • western blot knockout validation; human; 1:5000; loading ...; fig s2a
In order to investigate the antiviral mechanism of host factor and interferon-stimulated gene product, zinc-finger antiviral protein, BD Biosciences TRIM25 antibody (BD Biosciences, 610570) was used in western blot knockout validation on human samples at 1:5000 (fig s2a). PLoS Pathog (2017) ncbi
Articles Reviewed
  1. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  2. Chiang C, Pauli E, Biryukov J, Feister K, Meng M, White E, et al. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling. J Virol. 2018;92: pubmed publisher
  3. Meyerson N, Zhou L, Guo Y, Zhao C, Tao Y, Krug R, et al. Nuclear TRIM25 Specifically Targets Influenza Virus Ribonucleoproteins to Block the Onset of RNA Chain Elongation. Cell Host Microbe. 2017;22:627-638.e7 pubmed publisher
  4. Lang F, Aravamudhan S, Nolte H, Türk C, Hölper S, Muller S, et al. Dynamic changes in the mouse skeletal muscle proteome during denervation-induced atrophy. Dis Model Mech. 2017;10:881-896 pubmed publisher
  5. Hu Y, Li W, Gao T, Cui Y, Jin Y, Li P, et al. The Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Inhibits Type I Interferon Production by Interfering with TRIM25-Mediated RIG-I Ubiquitination. J Virol. 2017;91: pubmed publisher
  6. Li M, Lau Z, Cheung P, Aguilar E, Schneider W, Bozzacco L, et al. TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). PLoS Pathog. 2017;13:e1006145 pubmed publisher
  7. Torre S, Polyak M, Langlais D, Fodil N, Kennedy J, Radovanovic I, et al. USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation. Nat Immunol. 2017;18:54-63 pubmed publisher
  8. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  9. Radoshevich L, Impens F, Ribet D, Quereda J, Nam Tham T, Nahori M, et al. ISG15 counteracts Listeria monocytogenes infection. elife. 2015;4: pubmed publisher