This is a Validated Antibody Database (VAD) review about human TREM2, based on 18 published articles (read how Labome selects the articles), using TREM2 antibody in all methods. It is aimed to help Labome visitors find the most suited TREM2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TREM2 synonym: PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c

R&D Systems
domestic sheep polyclonal
  • immunohistochemistry; mouse; 1:150; fig 8c
R&D Systems TREM2 antibody (R&D Systems, AF1729) was used in immunohistochemistry on mouse samples at 1:150 (fig 8c). Acta Neuropathol Commun (2021) ncbi
domestic sheep polyclonal
  • immunohistochemistry; mouse; loading ...; fig 4j
R&D Systems TREM2 antibody (R&D, AF1729) was used in immunohistochemistry on mouse samples (fig 4j). Sci Adv (2021) ncbi
domestic sheep polyclonal
  • western blot; mouse; 1:400; loading ...; fig 6b
R&D Systems TREM2 antibody (R&D Systems, AF1729) was used in western blot on mouse samples at 1:400 (fig 6b). Acta Neuropathol Commun (2021) ncbi
domestic sheep polyclonal
  • immunohistochemistry; human; 1:50; loading ...; fig 1d
R&D Systems TREM2 antibody (R&D systems, BAF 1729) was used in immunohistochemistry on human samples at 1:50 (fig 1d). Nat Immunol (2021) ncbi
domestic sheep polyclonal
  • immunohistochemistry; human; 1:200; loading ...; fig 1d
R&D Systems TREM2 antibody (R&D systems, AF1729) was used in immunohistochemistry on human samples at 1:200 (fig 1d). Nat Immunol (2021) ncbi
domestic sheep polyclonal
  • western blot; mouse; fig 4a
R&D Systems TREM2 antibody (R&D, AF-1729) was used in western blot on mouse samples (fig 4a). Alzheimers Res Ther (2021) ncbi
domestic sheep polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2d
R&D Systems TREM2 antibody (R&D, AF1729) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 2d). Aging (Albany NY) (2020) ncbi
domestic sheep polyclonal
  • ELISA; mouse; 1:3000; loading ...; fig 6b, s2b
R&D Systems TREM2 antibody (R&D Systems, BAF1729) was used in ELISA on mouse samples at 1:3000 (fig 6b, s2b). Nat Commun (2019) ncbi
domestic goat polyclonal
  • ELISA; human; 1:3000; loading ...; fig 6b, s2b
R&D Systems TREM2 antibody (R&D Systems, BAF1828) was used in ELISA on human samples at 1:3000 (fig 6b, s2b). Nat Commun (2019) ncbi
domestic sheep polyclonal
  • immunocytochemistry; mouse; loading ...; fig 3d
R&D Systems TREM2 antibody (R&D Systems, AF1729) was used in immunocytochemistry on mouse samples (fig 3d). Neuron (2018) ncbi
domestic sheep polyclonal
  • ELISA; mouse; loading ...; fig 1a
In order to quantify soluble triggering receptor expressed on myeloid cells 2 and amyloid levels in Alzheimer's disease mouse model brains, R&D Systems TREM2 antibody (R&D Systems, BAF1729) was used in ELISA on mouse samples (fig 1a). Front Aging Neurosci (2017) ncbi
Abcam
rat monoclonal (RM0139-5J46)
  • western blot; mouse; 1:100
Abcam TREM2 antibody (Abcam, ab86491) was used in western blot on mouse samples at 1:100. Sci Adv (2022) ncbi
domestic rabbit monoclonal (EPR20243)
  • western blot; human; 1:500; fig 1g
Abcam TREM2 antibody (Abcam, EPR20243) was used in western blot on human samples at 1:500 (fig 1g). Alzheimers Res Ther (2020) ncbi
rat monoclonal (RM0139-5J46)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig ex8a
Abcam TREM2 antibody (Abcam, ab86491) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig ex8a). Nature (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-3)
  • ELISA; human; 1 ug/ml; fig 3f
Santa Cruz Biotechnology TREM2 antibody (Santa Cruz, 373828) was used in ELISA on human samples at 1 ug/ml (fig 3f). EMBO J (2022) ncbi
mouse monoclonal (B-3)
  • immunohistochemistry - frozen section; human; loading ...; fig 5c
Santa Cruz Biotechnology TREM2 antibody (SantaCruz, sc-373828) was used in immunohistochemistry - frozen section on human samples (fig 5c). J Exp Med (2017) ncbi
mouse monoclonal (B-3)
  • western blot; human; fig 1
Santa Cruz Biotechnology TREM2 antibody (Santa Cruz, sc-373828) was used in western blot on human samples (fig 1). PLoS ONE (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • proximity ligation assay; human; fig 5g
Invitrogen TREM2 antibody (Thermo Fisher, PA5-87933) was used in proximity ligation assay on human samples (fig 5g). Mol Neurodegener (2022) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8I4C)
  • immunoprecipitation; human; loading ...; fig 1a
Cell Signaling Technology TREM2 antibody (Cell Signaling Technology, 91068) was used in immunoprecipitation on human samples (fig 1a). Neuron (2018) ncbi
Fitzgerald Industries
  • western blot; human; 1:1000
In order to correlate TREM2 expression with neurodegenerative markers in Alzheimer's disease, Fitzgerald Industries TREM2 antibody (Fitzgerald, 10R-1115) was used in western blot on human samples at 1:1000. Brain Pathol (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; loading ...; fig 5c
MilliporeSigma TREM2 antibody (Sigma, HPA012571) was used in immunohistochemistry on human samples at 1:100 (fig 5c). Cell Mol Gastroenterol Hepatol (2018) ncbi
Articles Reviewed
  1. Singh N, Das B, Zhou J, Hu X, Yan R. Targeted BACE-1 inhibition in microglia enhances amyloid clearance and improved cognitive performance. Sci Adv. 2022;8:eabo3610 pubmed publisher
  2. Zhao P, Xu Y, Jiang L, Fan X, Ku Z, Li L, et al. LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Mol Neurodegener. 2022;17:44 pubmed publisher
  3. Reifschneider A, Robinson S, van Lengerich B, Gnörich J, Logan T, Heindl S, et al. Loss of TREM2 rescues hyperactivation of microglia, but not lysosomal deficits and neurotoxicity in models of progranulin deficiency. EMBO J. 2022;41:e109108 pubmed publisher
  4. Pankiewicz J, Lizińczyk A, Franco L, Díaz J, Martá Ariza M, Sadowski M. Absence of Apolipoprotein E is associated with exacerbation of prion pathology and promotes microglial neurodegenerative phenotype. Acta Neuropathol Commun. 2021;9:157 pubmed publisher
  5. Cheng J, Dong Y, Ma J, Pan R, Liao Y, Kong X, et al. Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology. Sci Adv. 2021;7: pubmed publisher
  6. Emre C, Do K, Jun B, Hjorth E, Alcalde S, Kautzmann M, et al. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease. Acta Neuropathol Commun. 2021;9:116 pubmed publisher
  7. Huang Y, Happonen K, Burrola P, O Connor C, Hah N, Huang L, et al. Microglia use TAM receptors to detect and engulf amyloid β plaques. Nat Immunol. 2021;22:586-594 pubmed publisher
  8. Pons V, Lévesque P, Plante M, Rivest S. Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer's disease. Alzheimers Res Ther. 2021;13:8 pubmed publisher
  9. Hall Roberts H, Agarwal D, Obst J, Smith T, Monzón Sandoval J, Di Daniel E, et al. TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages. Alzheimers Res Ther. 2020;12:151 pubmed publisher
  10. Wang Y, Lin Y, Wang L, Zhan H, Luo X, Zeng Y, et al. TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice. Aging (Albany NY). 2020;12:20862-20879 pubmed publisher
  11. Culemann S, Grüneboom A, Nicolás Ávila J, Weidner D, Lämmle K, Rothe T, et al. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature. 2019;572:670-675 pubmed publisher
  12. Zhong L, Xu Y, Zhuo R, Wang T, Wang K, Huang R, et al. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model. Nat Commun. 2019;10:1365 pubmed publisher
  13. Duggan S, Garry C, Behan F, Phipps S, Kudo H, Kirca M, et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol. 2018;5:569-590 pubmed publisher
  14. Zhao Y, Wu X, Li X, Jiang L, Gui X, Liu Y, et al. TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function. Neuron. 2018;97:1023-1031.e7 pubmed publisher
  15. Zhong L, Chen X, Wang T, Wang Z, Liao C, Wang Z, et al. Soluble TREM2 induces inflammatory responses and enhances microglial survival. J Exp Med. 2017;214:597-607 pubmed publisher
  16. Brendel M, Kleinberger G, Probst F, Jaworska A, Overhoff F, Blume T, et al. Increase of TREM2 during Aging of an Alzheimer's Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis. Front Aging Neurosci. 2017;9:8 pubmed publisher
  17. Bhattacharjee S, Zhao Y, Dua P, Rogaev E, Lukiw W. microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration. PLoS ONE. 2016;11:e0150211 pubmed publisher
  18. Lue L, Schmitz C, Serrano G, Sue L, Beach T, Walker D. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol. 2015;25:469-80 pubmed publisher