This is a Validated Antibody Database (VAD) review about human TOPBP1, based on 16 published articles (read how Labome selects the articles), using TOPBP1 antibody in all methods. It is aimed to help Labome visitors find the most suited TOPBP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TOPBP1 synonym: TOP2BP1; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; topoisomerase (DNA) II binding protein 1

Bethyl
rabbit polyclonal
  • western blot; human; loading ...; fig s15a
Bethyl TOPBP1 antibody (Bethyl Laboratories, A300-111A-1) was used in western blot on human samples (fig s15a). Science (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
Bethyl TOPBP1 antibody (Bethyl, A300-111A) was used in western blot on human samples at 1:1000 (fig 2). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 6
  • western blot; human; 1:1000; fig 3
Bethyl TOPBP1 antibody (Bethyl, A300-111A) was used in immunoprecipitation on human samples (fig 6) and in western blot on human samples at 1:1000 (fig 3). Viruses (2016) ncbi
rabbit polyclonal
  • western blot; chicken; 1:1000; fig 6
Bethyl TOPBP1 antibody (Bethyl Laboratories, A300-111A) was used in western blot on chicken samples at 1:1000 (fig 6). Oncotarget (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 1
Bethyl TOPBP1 antibody (Bethyl Laboratories, A300-111A) was used in immunocytochemistry on human samples at 1:200 (fig 1). J Cell Biol (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:5000; fig s4
In order to determine how PAXX interacts with Ku to promote DNA double-strand break repair, Bethyl TOPBP1 antibody (Bethyl Laboratories, A300-111A) was used in western blot on human samples at 1:5000 (fig s4). Science (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; human; loading ...; fig 2a, 2b
Abcam TOPBP1 antibody (Abcam, ab2402) was used in western blot on human samples (fig 2a, 2b). Cell Rep (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig s3
  • immunohistochemistry; human; 1:4000; fig s1
  • western blot; human; fig 1
In order to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer, Abcam TOPBP1 antibody (Abcam, ab2402) was used in immunoprecipitation on human samples (fig s3), in immunohistochemistry on human samples at 1:4000 (fig s1) and in western blot on human samples (fig 1). J Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig s5d
Abcam TOPBP1 antibody (Abcam, ab2402) was used in western blot on mouse samples (fig s5d). Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human
Abcam TOPBP1 antibody (Abcam, ab2402) was used in western blot on human samples . Biochem Biophys Res Commun (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam TOPBP1 antibody (Abcam, ab2402) was used in western blot on human samples . FEBS Lett (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-7)
  • immunocytochemistry; human; loading ...; fig 1d
Santa Cruz Biotechnology TOPBP1 antibody (Santa Cruz, B-7) was used in immunocytochemistry on human samples (fig 1d). Cell Rep (2016) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; human; fig 5d
Novus Biologicals TOPBP1 antibody (Novus Biologicals, NB100-217) was used in western blot on human samples (fig 5d). Nucleic Acids Res (2013) ncbi
BD Biosciences
mouse monoclonal (33/TopBP1)
  • immunocytochemistry; mouse; fig 7
BD Biosciences TOPBP1 antibody (BD Bioscience, 611875) was used in immunocytochemistry on mouse samples (fig 7). BMC Mol Biol (2016) ncbi
mouse monoclonal (33/TopBP1)
  • immunocytochemistry; mouse; fig 2
BD Biosciences TOPBP1 antibody (BD Bioscience, 611875) was used in immunocytochemistry on mouse samples (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (33/TopBP1)
  • flow cytometry; mouse
BD Biosciences TOPBP1 antibody (BD PharMingen, BP-1) was used in flow cytometry on mouse samples . Nat Immunol (2006) ncbi
Articles Reviewed
  1. Saldivar J, Hamperl S, Bocek M, Chung M, Bass T, Cisneros Soberanis F, et al. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018;361:806-810 pubmed publisher
  2. Karanika S, Karantanos T, Li L, Wang J, Park S, Yang G, et al. Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling. Cell Rep. 2017;18:1970-1981 pubmed publisher
  3. Yang C, Suzuki M, Yamakawa S, Uno S, Ishii A, Yamazaki S, et al. Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells. Nat Commun. 2016;7:12135 pubmed publisher
  4. Bristol M, Wang X, Smith N, Son M, Evans M, Morgan I. DNA Damage Reduces the Quality, but Not the Quantity of Human Papillomavirus 16 E1 and E2 DNA Replication. Viruses. 2016;8: pubmed publisher
  5. Kais Z, Rondinelli B, Holmes A, O Leary C, Kozono D, D Andrea A, et al. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair. Cell Rep. 2016;15:2488-99 pubmed publisher
  6. Cao N, Li J, Rao Y, Liu H, Wu J, Li B, et al. A potential role for protein palmitoylation and zDHHC16 in DNA damage response. BMC Mol Biol. 2016;17:12 pubmed publisher
  7. He D, Xiang J, Li B, Liu H. The dynamic behavior of Ect2 in response to DNA damage. Sci Rep. 2016;6:24504 pubmed publisher
  8. Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  9. Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
  10. Kliszczak M, Sedlackova H, Pitchai G, Streicher W, Krejci L, Hickson I. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget. 2015;6:40464-79 pubmed publisher
  11. Pedersen R, Kruse T, Nilsson J, Oestergaard V, Lisby M. TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. J Cell Biol. 2015;210:565-82 pubmed publisher
  12. Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  13. Fukumoto Y, Miura T, Morii M, Kubota S, Honda T, Kubota S, et al. v-Src inhibits the interaction between Rad17 and Rad9 and induces replication fork collapse. Biochem Biophys Res Commun. 2014;450:885-90 pubmed publisher
  14. Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res. 2013;41:6930-41 pubmed publisher
  15. Ding G, Liu H, Liang H, Ni R, Ding Z, Ni G, et al. HIF1-regulated ATRIP expression is required for hypoxia induced ATR activation. FEBS Lett. 2013;587:930-5 pubmed publisher
  16. Hu H, Wang B, Borde M, Nardone J, Maika S, Allred L, et al. Foxp1 is an essential transcriptional regulator of B cell development. Nat Immunol. 2006;7:819-26 pubmed