This is a Validated Antibody Database (VAD) review about human TOP2B, based on 10 published articles (read how Labome selects the articles), using TOP2B antibody in all methods. It is aimed to help Labome visitors find the most suited TOP2B antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TOP2B synonym: TOPIIB; top2beta; DNA topoisomerase 2-beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; U937 associated antigen; antigen MLAA-44; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb

R&D Systems
mouse monoclonal (681417)
  • other; mouse; 1:100; loading ...; fig 8j
  • western blot; mouse; 1:1000; loading ...; fig 8a
R&D Systems TOP2B antibody (R&D Systems, MAB6348) was used in other on mouse samples at 1:100 (fig 8j) and in western blot on mouse samples at 1:1000 (fig 8a). Nat Commun (2018) ncbi
mouse monoclonal (681417)
  • immunocytochemistry; human; fig 3
R&D Systems TOP2B antibody (R&D systems, MAB6348) was used in immunocytochemistry on human samples (fig 3). Biochem Pharmacol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-8)
  • immunohistochemistry; mouse; 1:300; loading ...; fig 4b
Santa Cruz Biotechnology TOP2B antibody (Santa Cruz, sc-25330) was used in immunohistochemistry on mouse samples at 1:300 (fig 4b). J Neurosci Res (2017) ncbi
mouse monoclonal (H-8)
  • western blot; human; 1:1000
Santa Cruz Biotechnology TOP2B antibody (Santa Cruz Biotechnology, sc-25330) was used in western blot on human samples at 1:1000. J Neuroimmunol (2014) ncbi
mouse monoclonal (H-8)
  • immunohistochemistry; human; fig 3
  • western blot; human; fig 3
  • immunohistochemistry; mouse; fig 3
  • western blot; mouse; fig 3
In order to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion, Santa Cruz Biotechnology TOP2B antibody (Santa Cruz, sc-25330) was used in immunohistochemistry on human samples (fig 3), in western blot on human samples (fig 3), in immunohistochemistry on mouse samples (fig 3) and in western blot on mouse samples (fig 3). Nucleic Acids Res (2014) ncbi
mouse monoclonal (H-8)
  • western blot; mouse
Santa Cruz Biotechnology TOP2B antibody (Santa Cruz, sc-25330) was used in western blot on mouse samples . Drug Discov Ther (2014) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Bethyl TOP2B antibody (Bethyl, A300-950A) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2017) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 10
  • western blot; human; fig 7
Bethyl TOP2B antibody (Bethyl, A300-950A) was used in chromatin immunoprecipitation on human samples (fig 10) and in western blot on human samples (fig 7). Nucleic Acids Res (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1e
In order to examine the role of topoisomerase II in mitotic clonal expansion and adipogenesis, Sigma-Aldrich TOP2B antibody (Sigma, HPA024120) was used in immunocytochemistry on mouse samples at 1:100 (fig 1b) and in western blot on mouse samples (fig 1e). Cell Signal (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:250; fig s11
Sigma-Aldrich TOP2B antibody (Prestige antibodies, Sigma, HPA024120) was used in western blot on human samples at 1:250 (fig s11). PLoS Genet (2015) ncbi
Articles Reviewed
  1. Hsu J, Xia W, Hsu Y, Chan L, Yu W, Cha J, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018;9:1908 pubmed publisher
  2. Li Y, Hao H, Swerdel M, Cho H, Lee K, Hart R, et al. Top2b is involved in the formation of outer segment and synapse during late-stage photoreceptor differentiation by controlling key genes of photoreceptor transcriptional regulatory network. J Neurosci Res. 2017;95:1951-1964 pubmed publisher
  3. Ahmad M, Shen W, Li W, Xue Y, Zou S, Xu D, et al. Topoisomerase 3? is the major topoisomerase for mRNAs and linked to neurodevelopment and mental dysfunction. Nucleic Acids Res. 2017;45:2704-2713 pubmed publisher
  4. Jacobsen R, Mazloumi Gavgani F, Mellgren G, Lewis A. DNA Topoisomerase II? contributes to the early steps of adipogenesis in 3T3-L1 cells. Cell Signal. 2016;28:1593-603 pubmed publisher
  5. Lee K, Bramley R, Cowell I, Jackson G, Austin C. Proteasomal inhibition potentiates drugs targeting DNA topoisomerase II. Biochem Pharmacol. 2016;103:29-39 pubmed publisher
  6. Aviner R, Shenoy A, Elroy Stein O, Geiger T. Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis. PLoS Genet. 2015;11:e1005554 pubmed publisher
  7. Kong L, Wei J, Haider A, Liebelt B, Ling X, Conrad C, et al. Therapeutic targets in subependymoma. J Neuroimmunol. 2014;277:168-75 pubmed publisher
  8. Fan A, Papadopoulos G, HOSSAIN M, Lin I, Hu J, Tang T, et al. Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress. Nucleic Acids Res. 2014;42:7625-41 pubmed publisher
  9. Zhang H, Zhang Y, Yasukawa T, Dalla Rosa I, Khiati S, Pommier Y. Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria. Nucleic Acids Res. 2014;42:7259-67 pubmed publisher
  10. Wang R, Wang Y, Gao Z, Qu X. The comparative study of acetyl-11-keto-beta-boswellic acid (AKBA) and aspirin in the prevention of intestinal adenomatous polyposis in APC(Min/+) mice. Drug Discov Ther. 2014;8:25-32 pubmed