This is a Validated Antibody Database (VAD) review about human TOP2A, based on 19 published articles (read how Labome selects the articles), using TOP2A antibody in all methods. It is aimed to help Labome visitors find the most suited TOP2A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TOP2A synonym: TOP2; TP2A

Abcam
domestic rabbit monoclonal (EP1102Y)
  • western blot; human; 1:1000; loading ...; fig 5d
Abcam TOP2A antibody (Abcam, ab52934) was used in western blot on human samples at 1:1000 (fig 5d). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit monoclonal (EP1102Y)
  • ChIP-Seq; mouse; ; loading ...; fig 4a
Abcam TOP2A antibody (Abcam, EP1102Y) was used in ChIP-Seq on mouse samples at (fig 4a). Nat Commun (2020) ncbi
domestic rabbit monoclonal (EP1102Y)
  • western blot; human; loading ...; fig s10c
Abcam TOP2A antibody (Abcam, EP1102Y) was used in western blot on human samples (fig s10c). Oncogene (2017) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunoprecipitation; mouse; loading ...; fig 7d
  • western blot; mouse; 1:3000; loading ...; fig 6a
  • western blot; human; 1:3000; loading ...; fig 6b
Abcam TOP2A antibody (Abcam, ab52934) was used in immunoprecipitation on mouse samples (fig 7d), in western blot on mouse samples at 1:3000 (fig 6a) and in western blot on human samples at 1:3000 (fig 6b). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 8a
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 8c
In order to address the effects of etoposide on germ cells in the developing ovary, Abcam TOP2A antibody (Abcam, ab52934) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 8a) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 8c). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (EP1102Y)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1e
In order to examine the role of topoisomerase II in mitotic clonal expansion and adipogenesis, Abcam TOP2A antibody (Abcam, ab52934) was used in immunocytochemistry on mouse samples at 1:100 (fig 1b) and in western blot on mouse samples (fig 1e). Cell Signal (2016) ncbi
domestic rabbit monoclonal (EPR5377)
  • western blot; human; 1:1000; loading ...; fig 3a
Abcam TOP2A antibody (Abcam, ab109524) was used in western blot on human samples at 1:1000 (fig 3a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (EPR5377)
  • immunocytochemistry; mouse; 1:200; fig 2
Abcam TOP2A antibody (Epitomics, 3747-1) was used in immunocytochemistry on mouse samples at 1:200 (fig 2). Nat Commun (2015) ncbi
domestic rabbit monoclonal (EP1102Y)
  • chromatin immunoprecipitation; mouse
Abcam TOP2A antibody (Abcam, ab52934) was used in chromatin immunoprecipitation on mouse samples . Cell Cycle (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-6)
  • immunocytochemistry; human; 1:100; loading ...; fig 1c
  • western blot; human; 1:500; loading ...; fig 1b
Santa Cruz Biotechnology TOP2A antibody (Santa Cruz, sc-166934) was used in immunocytochemistry on human samples at 1:100 (fig 1c) and in western blot on human samples at 1:500 (fig 1b). Proc Natl Acad Sci U S A (2020) ncbi
mouse monoclonal (A-8)
  • immunohistochemistry; human; loading ...; fig 1a
Santa Cruz Biotechnology TOP2A antibody (Santa Cruz, sc-165986) was used in immunohistochemistry on human samples (fig 1a). Oncogene (2018) ncbi
mouse monoclonal (G-6)
  • flow cytometry; human; fig 4
  • western blot; human; fig 4
Santa Cruz Biotechnology TOP2A antibody (Santa Cruz, sc-166934) was used in flow cytometry on human samples (fig 4) and in western blot on human samples (fig 4). Mol Med Rep (2015) ncbi
mouse monoclonal (F-12)
  • immunohistochemistry; mouse; fig 3
  • western blot; mouse; fig 3
In order to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion, Santa Cruz Biotechnology TOP2A antibody (Santa Cruz, sc-365916) was used in immunohistochemistry on mouse samples (fig 3) and in western blot on mouse samples (fig 3). Nucleic Acids Res (2014) ncbi
Dako
mouse monoclonal (Ki-S1)
  • western blot; human; fig 2
In order to study granzyme M-induced cell death, Dako TOP2A antibody (DAKO, Ki-S1) was used in western blot on human samples (fig 2). Cell Death Differ (2014) ncbi
BD Biosciences
mouse monoclonal (40/Topo II)
  • western blot; mouse; fig 3
BD Biosciences TOP2A antibody (BD Pharmingen, 611492) was used in western blot on mouse samples (fig 3). Cell Death Differ (2016) ncbi
mouse monoclonal (40/Topo II)
  • western blot; human; 1:1000; fig 7
BD Biosciences TOP2A antibody (BD Transduction Labs, 611493) was used in western blot on human samples at 1:1000 (fig 7). Cancers (Basel) (2015) ncbi
mouse monoclonal (31/Topo IIa)
  • ELISA; human; 1:500
BD Biosciences TOP2A antibody (BD Transduction Laboratories, 611326) was used in ELISA on human samples at 1:500. Nucleic Acids Res (2014) ncbi
MBL International
  • immunocytochemistry; human; loading ...; fig s2b
  • western blot; human; loading ...; fig 2b
  • western blot; mouse; loading ...; fig 2b
MBL International TOP2A antibody (MBL, M042-3) was used in immunocytochemistry on human samples (fig s2b), in western blot on human samples (fig 2b) and in western blot on mouse samples (fig 2b). Sci Rep (2015) ncbi
monoclonal
  • chromatin immunoprecipitation; human
MBL International TOP2A antibody (MBL, D081-1) was used in chromatin immunoprecipitation on human samples . Mol Biol Cell (2014) ncbi
Articles Reviewed
  1. Lei X, Cao K, Chen Y, Shen H, Liu Z, Qin H, et al. Nuclear Transglutaminase 2 interacts with topoisomerase II⍺ to promote DNA damage repair in lung cancer cells. J Exp Clin Cancer Res. 2021;40:224 pubmed publisher
  2. Nielsen C, Zhang T, Barisic M, Kalitsis P, Hudson D. Topoisomerase IIα is essential for maintenance of mitotic chromosome structure. Proc Natl Acad Sci U S A. 2020;117:12131-12142 pubmed publisher
  3. Alvarez Quilón A, Terron Bautista J, Delgado Sainz I, Serrano Benítez A, Romero Granados R, Martínez García P, et al. Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency. Nat Commun. 2020;11:910 pubmed publisher
  4. Fielding A, Concannon M, Darling S, Rusilowicz Jones E, Sacco J, Prior I, et al. The deubiquitylase USP15 regulates topoisomerase II alpha to maintain genome integrity. Oncogene. 2018;37:2326-2342 pubmed publisher
  5. Senturk J, Bohlman S, Manfredi J. Mdm2 selectively suppresses DNA damage arising from inhibition of topoisomerase II independent of p53. Oncogene. 2017;36:6085-6096 pubmed publisher
  6. Guturi K, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, et al. RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation. Nat Commun. 2016;7:12638 pubmed publisher
  7. Stefansdottir A, Johnston Z, Powles Glover N, Anderson R, Adams I, Spears N. Etoposide damages female germ cells in the developing ovary. BMC Cancer. 2016;16:482 pubmed publisher
  8. Jacobsen R, Mazloumi Gavgani F, Mellgren G, Lewis A. DNA Topoisomerase II? contributes to the early steps of adipogenesis in 3T3-L1 cells. Cell Signal. 2016;28:1593-603 pubmed publisher
  9. Morris J, Moseley V, Cabang A, Coleman K, Wei W, Garrett Mayer E, et al. Reduction in promotor methylation utilizing EGCG (epigallocatechin-3-gallate) restores RXR? expression in human colon cancer cells. Oncotarget. 2016;7:35313-26 pubmed publisher
  10. Barroso González J, Auclair S, Luan S, Thomas L, Atkins K, Aslan J, et al. PACS-2 mediates the ATM and NF-κB-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage. Cell Death Differ. 2016;23:1448-57 pubmed publisher
  11. Kang X, Song C, Du X, Zhang C, Liu Y, Liang L, et al. PTEN stabilizes TOP2A and regulates the DNA decatenation. Sci Rep. 2015;5:17873 pubmed publisher
  12. Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  13. Simpson D, Lemonie N, Morgan D, Gaddameedhi S, Kaufmann W. Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity. Cancers (Basel). 2015;7:1072-90 pubmed publisher
  14. Liu Y, Ban L, Su X, Gao S, Liu J, Cui X. Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells. Mol Med Rep. 2015;12:1598-604 pubmed publisher
  15. Kiianitsa K, Maizels N. Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay. Nucleic Acids Res. 2014;42:e108 pubmed publisher
  16. Zhang H, Zhang Y, Yasukawa T, Dalla Rosa I, Khiati S, Pommier Y. Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria. Nucleic Acids Res. 2014;42:7259-67 pubmed publisher
  17. d Alcontres M, Palacios J, Mejias D, Blasco M. TopoII? prevents telomere fragility and formation of ultra thin DNA bridges during mitosis through TRF1-dependent binding to telomeres. Cell Cycle. 2014;13:1463-81 pubmed publisher
  18. Gallego Paez L, Tanaka H, Bando M, Takahashi M, Nozaki N, Nakato R, et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell. 2014;25:302-17 pubmed publisher
  19. de Poot S, Lai K, van der Wal L, Plasman K, Van Damme P, Porter A, et al. Granzyme M targets topoisomerase II alpha to trigger cell cycle arrest and caspase-dependent apoptosis. Cell Death Differ. 2014;21:416-26 pubmed publisher