This is a Validated Antibody Database (VAD) review about human TNIK, based on 5 published articles (read how Labome selects the articles), using TNIK antibody in all methods. It is aimed to help Labome visitors find the most suited TNIK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TNIK synonym: MRT54; TRAF2 and NCK-interacting protein kinase

Invitrogen
rabbit polyclonal
  • western blot; human; fig 3
In order to elucidate a novel locus for intellectual disability due to a null mutation in TNIK, Invitrogen TNIK antibody (Thermo Fisher Scientific, PA5-15181) was used in western blot on human samples (fig 3). Hum Genet (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4b
In order to study the role of GAP and GEF complexing in GTP signaling, Bethyl TNIK antibody (Bethyl Laboratories, A302-695A) was used in western blot on mouse samples at 1:1000 (fig 4b). Sci Rep (2017) ncbi
BD Biosciences
mouse monoclonal (53/TNIK)
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, BD Biosciences TNIK antibody (BD Biosciences, 612250) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:400; fig 1
Sigma-Aldrich TNIK antibody (Sigma-Aldrich, HPA012128) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 1). BMC Cancer (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:1000
Sigma-Aldrich TNIK antibody (Sigma, HPA012297) was used in immunohistochemistry - free floating section on mouse samples at 1:1000. J Comp Neurol (2015) ncbi
Articles Reviewed
  1. Wilkinson B, Li J, Coba M. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep. 2017;7:5272 pubmed publisher
  2. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  3. Anazi S, Shamseldin H, Alnaqeb D, Abouelhoda M, Monies D, Salih M, et al. A null mutation in TNIK defines a novel locus for intellectual disability. Hum Genet. 2016;135:773-8 pubmed publisher
  4. Takahashi H, Ishikawa T, Ishiguro M, Okazaki S, Mogushi K, Kobayashi H, et al. Prognostic significance of Traf2- and Nck- interacting kinase (TNIK) in colorectal cancer. BMC Cancer. 2015;15:794 pubmed publisher
  5. Burette A, Phend K, Burette S, Lin Q, Liang M, Foltz G, et al. Organization of TNIK in dendritic spines. J Comp Neurol. 2015;523:1913-24 pubmed publisher