This is a Validated Antibody Database (VAD) review about human TNC, based on 15 published articles (read how Labome selects the articles), using TNC antibody in all methods. It is aimed to help Labome visitors find the most suited TNC antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TNC synonym: 150-225; DFNA56; GMEM; GP; HXB; JI; TN; TN-C

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D16C4)
  • immunocytochemistry; human; loading ...; fig 5g
  • western blot knockout validation; mouse; fig 3d
Cell Signaling Technology TNC antibody (Cell Signaling Technology, 12221) was used in immunocytochemistry on human samples (fig 5g) and in western blot knockout validation on mouse samples (fig 3d). Nat Commun (2020) ncbi
Abcam
mouse monoclonal (T2H5)
  • western blot; mouse; loading ...; fig 4g
Abcam TNC antibody (Abcam, ab3970) was used in western blot on mouse samples (fig 4g). Cell Res (2020) ncbi
domestic rabbit monoclonal (EPR4219)
  • western blot; mouse; loading ...; fig 4g
Abcam TNC antibody (Abcam, ab108930) was used in western blot on mouse samples (fig 4g). Cell Res (2020) ncbi
mouse monoclonal (EB2)
  • immunohistochemistry - frozen section; human; loading ...; fig 2
Abcam TNC antibody (Abcam, ab88280) was used in immunohistochemistry - frozen section on human samples (fig 2). Arch Toxicol (2020) ncbi
domestic rabbit monoclonal (EPR4219)
  • western blot; mouse; 1:1000; loading ...; fig 1, 2, 3, 5, 6, 7, 8
Abcam TNC antibody (Abcam, Cambridge, UK, #AB108930) was used in western blot on mouse samples at 1:1000 (fig 1, 2, 3, 5, 6, 7, 8). Sci Rep (2020) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunohistochemistry - paraffin section; mouse; 1:4000; loading ...; fig 3b
Abcam TNC antibody (Abcam, EPR4219) was used in immunohistochemistry - paraffin section on mouse samples at 1:4000 (fig 3b). Cancers (Basel) (2020) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunocytochemistry; human; fig 5f
Abcam TNC antibody (Abcam, Ab108930) was used in immunocytochemistry on human samples (fig 5f). Cell Stem Cell (2017) ncbi
mouse monoclonal (T2H5)
  • western blot; human; 1:50; loading ...; fig 2a
Abcam TNC antibody (Abcam, 3970) was used in western blot on human samples at 1:50 (fig 2a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunohistochemistry - frozen section; human; 1:500; loading ...
In order to examine Sonic hedgehog signaling in the human fetal neocortex, Abcam TNC antibody (Abcam, ab108930) was used in immunohistochemistry - frozen section on human samples at 1:500. Nat Neurosci (2016) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunohistochemistry; mouse; 1:500; fig 2
In order to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors, Abcam TNC antibody (Abcam, AB108930) was used in immunohistochemistry on mouse samples at 1:500 (fig 2). Nat Med (2016) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunohistochemistry - paraffin section; human; fig 2
  • flow cytometry; human; fig 5
  • western blot; human; fig 1
Abcam TNC antibody (Abcam, ab108930) was used in immunohistochemistry - paraffin section on human samples (fig 2), in flow cytometry on human samples (fig 5) and in western blot on human samples (fig 1). J Proteome Res (2015) ncbi
domestic rabbit monoclonal (EPR4219)
  • western blot; human; 1:1000
Abcam TNC antibody (Abcam, ab108930) was used in western blot on human samples at 1:1000. Cancer Res (2015) ncbi
domestic rabbit monoclonal (EPR4219)
  • immunocytochemistry; human
  • western blot; human
Abcam TNC antibody (Abcam, ab108930) was used in immunocytochemistry on human samples and in western blot on human samples . J Am Soc Nephrol (2014) ncbi
R&D Systems
rat monoclonal (578)
  • other; mouse; loading ...
R&D Systems TNC antibody (R&D Systems, 578) was used in other on mouse samples . Nat Commun (2020) ncbi
rat monoclonal (578)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1b
R&D Systems TNC antibody (R&D Systems, MAB2138) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1b). Front Physiol (2020) ncbi
Invitrogen
mouse monoclonal (4C8MS)
  • immunohistochemistry; rat; 1:100; loading ...
Invitrogen TNC antibody (Thermo Scientific, MA5-16086) was used in immunohistochemistry on rat samples at 1:100. Biol Proced Online (2020) ncbi
Novus Biologicals
mouse monoclonal (4C8MS)
  • western blot; human; 1:1000; loading ...; fig 1b
Novus Biologicals TNC antibody (Novus Biologicals, NB110-68136) was used in western blot on human samples at 1:1000 (fig 1b). Nat Commun (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D16C4)
  • western blot; human; loading ...; fig 3e
Cell Signaling Technology TNC antibody (Cell Signaling, 12221) was used in western blot on human samples (fig 3e). Ther Adv Urol (2021) ncbi
domestic rabbit monoclonal (D16C4)
  • immunocytochemistry; human; loading ...; fig 5g
  • western blot knockout validation; mouse; fig 3d
Cell Signaling Technology TNC antibody (Cell Signaling Technology, 12221) was used in immunocytochemistry on human samples (fig 5g) and in western blot knockout validation on mouse samples (fig 3d). Nat Commun (2020) ncbi
Articles Reviewed
  1. Kareddula A, Medina D, Petrosky W, Dolfi S, Tereshchenko I, Walton K, et al. The role of chromodomain helicase DNA binding protein 1 (CHD1) in promoting an invasive prostate cancer phenotype. Ther Adv Urol. 2021;13:17562872211022462 pubmed publisher
  2. Zhou S, Zhang W, Cai G, Ding Y, Wei C, Li S, et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res. 2020;30:1063-1077 pubmed publisher
  3. Li Z, Zhang H, Huang Y, Huang J, Sun P, Zhou N, et al. Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation. Nat Commun. 2020;11:3806 pubmed publisher
  4. Hering H, Zoschke C, Kühn M, Gadicherla A, Weindl G, Luch A, et al. TatS: a novel in vitro tattooed human skin model for improved pigment toxicology research. Arch Toxicol. 2020;94:2423-2434 pubmed publisher
  5. Ohki S, Oka K, Ogata K, Okuhara S, Rikitake M, Toda Nakamura M, et al. Transforming Growth Factor-Beta and Sonic Hedgehog Signaling in Palatal Epithelium Regulate Tenascin-C Expression in Palatal Mesenchyme During Soft Palate Development. Front Physiol. 2020;11:532 pubmed publisher
  6. Gremlich S, Roth Kleiner M, Equey L, Fytianos K, Schittny J, Cremona T. Tenascin-C inactivation impacts lung structure and function beyond lung development. Sci Rep. 2020;10:5118 pubmed publisher
  7. Aaltonen N, Singha P, Jakupović H, Wirth T, Samaranayake H, Pasonen Seppänen S, et al. High-Resolution Confocal Fluorescence Imaging of Serine Hydrolase Activity in Cryosections - Application to Glioma Brain Unveils Activity Hotspots Originating from Tumor-Associated Neutrophils. Biol Proced Online. 2020;22:6 pubmed publisher
  8. Medeiros B, Goodale D, Postenka C, Lowes L, Kiser P, Hearn S, et al. Triple-Negative Primary Breast Tumors Induce Supportive Premetastatic Changes in the Extracellular Matrix and Soluble Components of the Lung Microenvironment. Cancers (Basel). 2020;12: pubmed publisher
  9. Bershteyn M, Nowakowski T, Pollen A, Di Lullo E, Nene A, Wynshaw Boris A, et al. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia. Cell Stem Cell. 2017;20:435-449.e4 pubmed publisher
  10. Bhattacharyya S, Wang W, Morales Nebreda L, Feng G, Wu M, Zhou X, et al. Tenascin-C drives persistence of organ fibrosis. Nat Commun. 2016;7:11703 pubmed publisher
  11. Wang L, Hou S, Han Y. Hedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex. Nat Neurosci. 2016;19:888-96 pubmed publisher
  12. Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
  13. Nie S, Gurrea M, Zhu J, Thakolwiboon S, Heth J, Muraszko K, et al. Tenascin-C: a novel candidate marker for cancer stem cells in glioblastoma identified by tissue microarrays. J Proteome Res. 2015;14:814-22 pubmed publisher
  14. Lee K, Yeo S, Sung C, Kim S. Twist1 is a key regulator of cancer-associated fibroblasts. Cancer Res. 2015;75:73-85 pubmed publisher
  15. Byron A, Randles M, Humphries J, Mironov A, Hamidi H, Harris S, et al. Glomerular cell cross-talk influences composition and assembly of extracellular matrix. J Am Soc Nephrol. 2014;25:953-66 pubmed publisher