This is a Validated Antibody Database (VAD) review about human TIMP-2, based on 17 published articles (read how Labome selects the articles), using TIMP-2 antibody in all methods. It is aimed to help Labome visitors find the most suited TIMP-2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TIMP-2 synonym: CSC-21K; DDC8

Knockout validation
R&D Systems
domestic goat polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig s7e
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig s7e
R&D Systems TIMP-2 antibody (R&D Systems, AF971) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig s7e) and in immunohistochemistry - free floating section on mouse samples at 1:100 (fig s7e). Nature (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D18B7)
  • western blot knockout validation; mouse; 1:500; loading ...; fig s7c
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, D18B7) was used in western blot knockout validation on mouse samples at 1:500 (fig s7c). Nature (2017) ncbi
Invitrogen
mouse monoclonal (F27 P3 A4)
  • western blot; mouse; loading ...; fig 4b
Invitrogen TIMP-2 antibody (Thermo Fisher Scientific, MA1-774) was used in western blot on mouse samples (fig 4b). Molecules (2019) ncbi
mouse monoclonal (T2-101)
  • other; human; loading ...; fig s1
In order to investigate the mechanisms of toxicity by the negative allosteric modulators of metabotropic glutamate receptor 5, Invitrogen TIMP-2 antibody (Thermo Fisher, MS-571-PABX) was used in other on human samples (fig s1). Cell Chem Biol (2017) ncbi
mouse monoclonal (F27 P3 A4)
  • immunohistochemistry - paraffin section; human; loading ...; fig 6c
In order to investigate the oncogenic functions of CHD4, Invitrogen TIMP-2 antibody (Thermo Fisher Scientific, MA1-774) was used in immunohistochemistry - paraffin section on human samples (fig 6c). Cancer Cell (2017) ncbi
mouse monoclonal (F27 P3 A4)
  • western blot; mouse; 1:2000; loading ...; fig s5a
In order to determine the impact of smooth muscle cell beta-catenin to vascular homeostasis and arterial injury, Invitrogen TIMP-2 antibody (Thermo fisher, MA1-774) was used in western blot on mouse samples at 1:2000 (fig s5a). Arterioscler Thromb Vasc Biol (2017) ncbi
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
In order to study the clinical significance and expression of metalloproteases and their inhibitors in invasive breast carcinoma endothelial cells, Invitrogen TIMP-2 antibody (Thermo, MA5-12207) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 2). Clin Breast Cancer (2016) ncbi
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; human; fig 2
In order to investigate the roles of MMP-9 and TIMP-2 in regulating tumor progression in ameloblastomas, Invitrogen TIMP-2 antibody (Thermo Scientific, 3A4) was used in immunohistochemistry - paraffin section on human samples (fig 2). Appl Immunohistochem Mol Morphol (2016) ncbi
mouse monoclonal (3A4)
  • immunohistochemistry; human; 1:200
Invitrogen TIMP-2 antibody (Thermo Fisher Scientific, 3A4) was used in immunohistochemistry on human samples at 1:200. BMC Cancer (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6a
Santa Cruz Biotechnology TIMP-2 antibody (Santa Cruz, sc-21735) was used in immunohistochemistry - paraffin section on mouse samples (fig 6a). Cardiovasc Diabetol (2018) ncbi
mouse monoclonal (B-12)
  • western blot; human; fig 5a
Santa Cruz Biotechnology TIMP-2 antibody (Santa Cruz, sc-365671) was used in western blot on human samples (fig 5a). J Am Heart Assoc (2016) ncbi
mouse monoclonal (B-12)
  • western blot; rat; 1:500; fig 8
Santa Cruz Biotechnology TIMP-2 antibody (Santa Cruz, SC365671) was used in western blot on rat samples at 1:500 (fig 8). Int J Mol Med (2015) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2b
R&D Systems TIMP-2 antibody (R&D systems, AF971) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 2b). Sci Adv (2021) ncbi
domestic goat polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig s7e
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig s7e
R&D Systems TIMP-2 antibody (R&D Systems, AF971) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig s7e) and in immunohistochemistry - free floating section on mouse samples at 1:100 (fig s7e). Nature (2017) ncbi
Abcam
mouse monoclonal (3A4)
  • western blot; human; loading ...; fig s3a-b
In order to investigate the oncogenic functions of CHD4, Abcam TIMP-2 antibody (Abcam, ab1828) was used in western blot on human samples (fig s3a-b). Cancer Cell (2017) ncbi
mouse monoclonal (3A4)
  • western blot; domestic rabbit; 1:1000; loading ...; fig 6b
Abcam TIMP-2 antibody (Abcam, ab1828) was used in western blot on domestic rabbit samples at 1:1000 (fig 6b). Int J Mol Med (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D18B7)
  • western blot; mouse; 1:2000; loading ...; fig 2b
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, 5738) was used in western blot on mouse samples at 1:2000 (fig 2b). J Cell Sci (2022) ncbi
domestic rabbit monoclonal (D18B7)
  • western blot; mouse; loading ...; fig s2
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, D18B7) was used in western blot on mouse samples (fig s2). Stem Cell Res Ther (2019) ncbi
domestic rabbit monoclonal (D18B7)
  • western blot; mouse; 1:1000; loading ...; fig 1c
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, D18B7) was used in western blot on mouse samples at 1:1000 (fig 1c). J Exp Med (2017) ncbi
domestic rabbit monoclonal (D18B7)
  • western blot knockout validation; mouse; 1:500; loading ...; fig s7c
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, D18B7) was used in western blot knockout validation on mouse samples at 1:500 (fig s7c). Nature (2017) ncbi
domestic rabbit monoclonal (D18B7)
  • western blot; human; 1:1000; loading ...; fig 5a
Cell Signaling Technology TIMP-2 antibody (Cell Signaling, 5738) was used in western blot on human samples at 1:1000 (fig 5a). PLoS ONE (2016) ncbi
Articles Reviewed
  1. Pantasis S, Friemel J, Brütsch S, Hu Z, Krautbauer S, Liebisch G, et al. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation. J Cell Sci. 2022;135: pubmed publisher
  2. Zarb Y, Sridhar S, Nassiri S, Utz S, Schaffenrath J, Maheshwari U, et al. Microglia control small vessel calcification via TREM2. Sci Adv. 2021;7: pubmed publisher
  3. Lu D, Liao Y, Zhu S, Chen Q, Xie D, Liao J, et al. Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction. Stem Cell Res Ther. 2019;10:127 pubmed publisher
  4. Yi R, Zhang J, Sun P, Qian Y, Zhao X. Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice. Molecules. 2019;24: pubmed publisher
  5. Nasiri Ansari Ν, Dimitriadis G, Agrogiannis G, Perrea D, Kostakis I, Kaltsas G, et al. Canagliflozin attenuates the progression of atherosclerosis and inflammation process in APOE knockout mice. Cardiovasc Diabetol. 2018;17:106 pubmed publisher
  6. Khrimian L, Obri A, Ramos Brossier M, Rousseaud A, Moriceau S, Nicot A, et al. Gpr158 mediates osteocalcin's regulation of cognition. J Exp Med. 2017;214:2859-2873 pubmed publisher
  7. Shah F, Stepan A, O Mahony A, Velichko S, Folias A, Houle C, et al. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators. Cell Chem Biol. 2017;24:858-869.e5 pubmed publisher
  8. Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
  9. Castellano J, Mosher K, Abbey R, McBride A, James M, Berdnik D, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017;544:488-492 pubmed publisher
  10. Riascos Bernal D, Chinnasamy P, Gross J, Almonte V, Egaña Gorroño L, Parikh D, et al. Inhibition of Smooth Muscle ?-Catenin Hinders Neointima Formation After Vascular Injury. Arterioscler Thromb Vasc Biol. 2017;37:879-888 pubmed publisher
  11. Liu J, Hu F, Tang J, Tang S, Xia K, Wu S, et al. Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Int J Mol Med. 2017;39:879-888 pubmed publisher
  12. Du M, Wang X, Tan X, Li X, Huang D, Huang K, et al. Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed
  13. Cid S, Eiró N, Gonzalez L, Béridze N, Vazquez J, Vizoso F. Expression and Clinical Significance of Metalloproteases and Their Inhibitors by Endothelial Cells From Invasive Breast Carcinomas. Clin Breast Cancer. 2016;16:e83-91 pubmed publisher
  14. Grecka E, Statkiewicz M, Gorska A, Biernacka M, Grygorowicz M, Masnyk M, et al. Prenyl Ammonium Salts--New Carriers for Gene Delivery: A B16-F10 Mouse Melanoma Model. PLoS ONE. 2016;11:e0153633 pubmed publisher
  15. Yan G, Wang Q, Hu S, Wang D, Qiao Y, Ma G, et al. Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury. Int J Mol Med. 2015;36:1001-11 pubmed publisher
  16. Nunia K, Urs A, Kumar P. Interplay Between MMP-9 and TIMP-2 Regulates Ameloblastoma Behavior and Tooth Morphogenesis. Appl Immunohistochem Mol Morphol. 2016;24:364-72 pubmed publisher
  17. Kim G, Lee J, Choi Y, Lee K, Lee J, Nam J, et al. Expression of matrix metalloproteinases and their inhibitors in different immunohistochemical-based molecular subtypes of breast cancer. BMC Cancer. 2014;14:959 pubmed publisher