This is a Validated Antibody Database (VAD) review about human TCF3, based on 13 published articles (read how Labome selects the articles), using TCF3 antibody in all methods. It is aimed to help Labome visitors find the most suited TCF3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TCF3 synonym: AGM8; E2A; E47; ITF1; TCF-3; VDIR; bHLHb21; p75

Santa Cruz Biotechnology
mouse monoclonal (Yae)
  • immunoprecipitation; human; 1:225; loading ...; fig 3h
  • western blot; human; 1:200; loading ...; fig 3h
  • western blot; mouse; 1:200; loading ...; fig 3b
Santa Cruz Biotechnology TCF3 antibody (Santa Cruz, sc-416) was used in immunoprecipitation on human samples at 1:225 (fig 3h), in western blot on human samples at 1:200 (fig 3h) and in western blot on mouse samples at 1:200 (fig 3b). elife (2019) ncbi
mouse monoclonal (Yae)
Santa Cruz Biotechnology TCF3 antibody (santa Cruz, sc-416) was used . PLoS ONE (2015) ncbi
mouse monoclonal (G-2)
  • chromatin immunoprecipitation; human
Santa Cruz Biotechnology TCF3 antibody (Santa Cruz, sc-133075 X) was used in chromatin immunoprecipitation on human samples . Biochem Cell Biol (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D2B1)
  • ChIP-Seq; human; 1-2 ug / 500 ul chr; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Cell Signaling Technology TCF3 antibody (Cell Signaling, 12258) was used in ChIP-Seq on human samples at 1-2 ug / 500 ul chr (fig 4c) and in chromatin immunoprecipitation on human samples (fig 4c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (D2B1)
  • ChIP-Seq; human; loading ...; fig 5a
Cell Signaling Technology TCF3 antibody (Cell Signaling, 12258) was used in ChIP-Seq on human samples (fig 5a). Exp Hematol (2017) ncbi
domestic rabbit monoclonal (D2B1)
  • flow cytometry; mouse; loading ...; fig s7g
  • flow cytometry; human; fig s9g
In order to explore the role of exhausted CD8 positive CXCR5 positive T cells in mice chronically infected with lymphocytic choriomeningitis virus, Cell Signaling Technology TCF3 antibody (Cell Signaling, D2B1) was used in flow cytometry on mouse samples (fig s7g) and in flow cytometry on human samples (fig s9g). Nature (2016) ncbi
domestic rabbit monoclonal (D15G11)
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 2
Cell Signaling Technology TCF3 antibody (Cell Signaling, 2883) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 2). Reproduction (2015) ncbi
domestic rabbit monoclonal (D15G11)
  • western blot; human; 1:1000; fig 9c
In order to describe a SUMO-specific protease-based methodology suitable for mass spectroscopy, Cell Signaling Technology TCF3 antibody (Cell Signaling Technology, 2883P) was used in western blot on human samples at 1:1000 (fig 9c). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D15G11)
  • western blot; human; 1:1000
Cell Signaling Technology TCF3 antibody (Cell Signaling, D15G11) was used in western blot on human samples at 1:1000. BMC Cell Biol (2013) ncbi
BD Biosciences
mouse monoclonal (G127-32)
  • chromatin immunoprecipitation; mouse; loading ...; fig 7b
BD Biosciences TCF3 antibody (BD Biosciences, G127-32) was used in chromatin immunoprecipitation on mouse samples (fig 7b). PLoS Genet (2017) ncbi
mouse monoclonal (G127-32)
  • immunoprecipitation; human; fig 5
BD Biosciences TCF3 antibody (BD Biosciences, 554077) was used in immunoprecipitation on human samples (fig 5). Biomed Res Int (2016) ncbi
mouse monoclonal (G127-32)
  • immunocytochemistry; human; 1:100
In order to test if altering the transcription programming in pancreatic ductal adenocarcinomas can revert these cancerous cells back to quiescent acinar cells, BD Biosciences TCF3 antibody (BD Pharmingen, 554077) was used in immunocytochemistry on human samples at 1:100. Pancreas (2015) ncbi
mouse monoclonal (G127-32)
  • western blot; mouse; fig 3
BD Biosciences TCF3 antibody (BD Biosciences, 554077) was used in western blot on mouse samples (fig 3). BMC Dev Biol (2009) ncbi
Articles Reviewed
  1. Senigl F, Maman Y, Dinesh R, Alinikula J, Seth R, Pecnova L, et al. Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation. Cell Rep. 2019;29:3902-3915.e8 pubmed publisher
  2. Blomfield I, Rocamonde B, Masdeu M, Mulugeta E, Vaga S, van den Berg D, et al. Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells. elife. 2019;8: pubmed publisher
  3. Kurkewich J, Hansen J, Klopfenstein N, Zhang H, Wood C, Boucher A, et al. The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis. PLoS Genet. 2017;13:e1006887 pubmed publisher
  4. Godfrey L, Kerry J, Thorne R, Repapi E, Davies J, Tapia M, et al. MLL-AF4 binds directly to a BCL-2 specific enhancer and modulates H3K27 acetylation. Exp Hematol. 2017;47:64-75 pubmed publisher
  5. He R, Hou S, Liu C, Zhang A, Bai Q, Han M, et al. Follicular CXCR5- expressing CD8(+) T cells curtail chronic viral infection. Nature. 2016;537:412-428 pubmed publisher
  6. Rogers J, Owens K, Kurkewich J, Klopfenstein N, Iyer S, Simon M, et al. E2A Antagonizes PU.1 Activity through Inhibition of DNA Binding. Biomed Res Int. 2016;2016:3983686 pubmed publisher
  7. Xiong W, Zhang L, Yu L, Xie W, Man Y, Xiong Y, et al. Estradiol promotes cells invasion by activating β-catenin signaling pathway in endometriosis. Reproduction. 2015;150:507-16 pubmed publisher
  8. Woodcroft M, Nanan K, Thompson P, Tyryshkin K, Smith S, Slany R, et al. Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors. PLoS ONE. 2015;10:e0130495 pubmed publisher
  9. Hendriks I, D Souza R, Chang J, Mann M, Vertegaal A. System-wide identification of wild-type SUMO-2 conjugation sites. Nat Commun. 2015;6:7289 pubmed publisher
  10. Kim S, Lahmy R, Riha C, Yang C, Jakubison B, van Niekerk J, et al. The basic helix-loop-helix transcription factor E47 reprograms human pancreatic cancer cells to a quiescent acinar state with reduced tumorigenic potential. Pancreas. 2015;44:718-27 pubmed publisher
  11. Cázares Ordoñez V, González Duarte R, Díaz L, Ishizawa M, Uno S, Ortíz V, et al. A cis-acting element in the promoter of human ether à go-go 1 potassium channel gene mediates repression by calcitriol in human cervical cancer cells. Biochem Cell Biol. 2015;93:94-101 pubmed publisher
  12. Ogunkolade B, Jones T, Aarum J, Szary J, Owen N, Ottaviani D, et al. BORIS/CTCFL is an RNA-binding protein that associates with polysomes. BMC Cell Biol. 2013;14:52 pubmed publisher
  13. Jones M, Kondo M, Zhuang Y. A tamoxifen inducible knock-in allele for investigation of E2A function. BMC Dev Biol. 2009;9:51 pubmed publisher