This is a Validated Antibody Database (VAD) review about human Smac, based on 18 published articles (read how Labome selects the articles), using Smac antibody in all methods. It is aimed to help Labome visitors find the most suited Smac antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Smac synonym: DFNA64; SMAC

Knockout validation
Abcam
domestic rabbit monoclonal (Y12)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4f
Abcam Smac antibody (Abcam, 32023) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4f). Nat Cell Biol (2017) ncbi
Cell Signaling Technology
mouse monoclonal
  • immunohistochemistry knockout validation; human; loading ...; fig s1c
  • western blot knockout validation; human; loading ...; fig s1c
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
Cell Signaling Technology Smac antibody (Cell Signalling, 2954) was used in immunohistochemistry knockout validation on human samples (fig s1c), in western blot knockout validation on human samples (fig s1c) and in immunohistochemistry - paraffin section on human samples (fig 1a). Cell Death Dis (2020) ncbi
Abcam
domestic rabbit monoclonal (Y12)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4f
Abcam Smac antibody (Abcam, 32023) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4f). Nat Cell Biol (2017) ncbi
domestic rabbit monoclonal (Y12)
  • immunohistochemistry - paraffin section; human; 1:400; fig 6
  • western blot; human; 1:1000; fig 1
Abcam Smac antibody (abcam, ab32023) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 6) and in western blot on human samples at 1:1000 (fig 1). Int J Oncol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam Smac antibody (Abcam, ab8115) was used in western blot on human samples at 1:1000 (fig 1a). Mitochondrion (2016) ncbi
Novus Biologicals
mouse monoclonal (78-1-118)
  • western blot; mouse; fig 1
In order to study the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome and mitochondrial-targeted aryl hydrocarbon receptor, Novus Biologicals Smac antibody (Novus Biologicals, NB500-213) was used in western blot on mouse samples (fig 1). Toxicol Appl Pharmacol (2016) ncbi
Cell Signaling Technology
mouse monoclonal
  • western blot; mouse; 1:1000; fig 3e
Cell Signaling Technology Smac antibody (Cell Signaling, 2954) was used in western blot on mouse samples at 1:1000 (fig 3e). Nat Commun (2022) ncbi
mouse monoclonal
  • immunohistochemistry knockout validation; human; loading ...; fig s1c
  • western blot knockout validation; human; loading ...; fig s1c
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
Cell Signaling Technology Smac antibody (Cell Signalling, 2954) was used in immunohistochemistry knockout validation on human samples (fig s1c), in western blot knockout validation on human samples (fig s1c) and in immunohistochemistry - paraffin section on human samples (fig 1a). Cell Death Dis (2020) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 9
Cell Signaling Technology Smac antibody (Cell Signaling Technology, 2954S) was used in western blot on human samples (fig 9). Biomolecules (2019) ncbi
domestic rabbit monoclonal (D5S3R)
  • immunocytochemistry; human; loading ...; fig s5j
Cell Signaling Technology Smac antibody (Cell Signaling Technology, 15108) was used in immunocytochemistry on human samples (fig s5j). Cell (2019) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology Smac antibody (Cell Signaling, 2954) was used in western blot on human samples (fig 3d). Mol Cell (2019) ncbi
mouse monoclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Smac antibody (Cell Signaling, 2954) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 5
Cell Signaling Technology Smac antibody (Cell Signaling Technology, 2954) was used in western blot on human samples (fig 5). Anticancer Res (2017) ncbi
mouse monoclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology Smac antibody (CST, 2954) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
mouse monoclonal
  • western blot; human; loading ...
In order to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches, Cell Signaling Technology Smac antibody (Cell Signaling Technology, 2954) was used in western blot on human samples . Cell Syst (2017) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology Smac antibody (Cell Signaling, 2954) was used in western blot on human samples (fig 1c). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D5S3R)
  • immunohistochemistry; human; fig 2
In order to elucidate the intrinsic and extrinsic apoptotic pathways of megakaryocytes in human the intrinsic and extrinsic apoptotic pathways of megakaryocytes in human, Cell Signaling Technology Smac antibody (Cell Signaling, D5S3R) was used in immunohistochemistry on human samples (fig 2). J Clin Pathol (2016) ncbi
mouse monoclonal
  • western blot; human; 1:1000; tbl 1
Cell Signaling Technology Smac antibody (Cell Signaling, 2954) was used in western blot on human samples at 1:1000 (tbl 1). Methods Mol Biol (2015) ncbi
BD Biosciences
mouse (7)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Smac antibody (BD, 7) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
ProSci
domestic rabbit polyclonal
  • western blot; human; fig 2
In order to study placental mammal-specific mitochondrial pyruvate carrier subunit called MPC1-like and its expression in post-meiotic male germ cells, ProSci Smac antibody (ProSci, 2409) was used in western blot on human samples (fig 2). J Biol Chem (2016) ncbi
Articles Reviewed
  1. Liu W, Zhou H, Wang H, Zhang Q, Zhang R, Willard B, et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun. 2022;13:2748 pubmed publisher
  2. Guttà C, Rahman A, Aura C, Dynoodt P, Charles E, Hirschenhahn E, et al. Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma. Cell Death Dis. 2020;11:124 pubmed publisher
  3. Thangaraj K, Balasubramanian B, Park S, Natesan K, Liu W, Manju V. Orientin Induces G0/G1 Cell Cycle Arrest and Mitochondria Mediated Intrinsic Apoptosis in Human Colorectal Carcinoma HT29 Cells. Biomolecules. 2019;9: pubmed publisher
  4. Zierhut C, Yamaguchi N, Paredes M, Luo J, Carroll T, Funabiki H. The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Cell. 2019;178:302-315.e23 pubmed publisher
  5. Lee S, Mayr C. Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation. Mol Cell. 2019;: pubmed publisher
  6. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  7. Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
  8. Jelinek M, Kabelova A, Srámek J, Seitz J, Ojima I, Kovar J. Differing Mechanisms of Death Induction by Fluorinated Taxane SB-T-12854 in Breast Cancer Cells. Anticancer Res. 2017;37:1581-1590 pubmed
  9. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  10. Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
  11. Qin S, Yang C, Zhang B, Li X, Sun X, Li G, et al. XIAP inhibits mature Smac-induced apoptosis by degrading it through ubiquitination in NSCLC. Int J Oncol. 2016;49:1289-96 pubmed publisher
  12. Weyhenmeyer B, Noonan J, Würstle M, Lincoln F, Johnston G, Rehm M, et al. Predicting the cell death responsiveness and sensitization of glioma cells to TRAIL and temozolomide. Oncotarget. 2016;7:61295-61311 pubmed publisher
  13. Vanderperre B, Cermakova K, Escoffier J, Kaba M, Bender T, Nef S, et al. MPC1-like Is a Placental Mammal-specific Mitochondrial Pyruvate Carrier Subunit Expressed in Postmeiotic Male Germ Cells. J Biol Chem. 2016;291:16448-61 pubmed publisher
  14. Shephard F, Greville Heygate O, Liddell S, Emes R, Chakrabarti L. Analysis of Mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion. 2016;29:45-52 pubmed publisher
  15. Hwang H, Dornbos P, Steidemann M, Dunivin T, Rizzo M, LaPres J. Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome. Toxicol Appl Pharmacol. 2016;304:121-32 pubmed publisher
  16. Malherbe J, Fuller K, Mirzai B, Kavanagh S, So C, Ip H, et al. Dysregulation of the intrinsic apoptotic pathway mediates megakaryocytic hyperplasia in myeloproliferative neoplasms. J Clin Pathol. 2016;: pubmed publisher
  17. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  18. Vega Naredo I, Cunha Oliveira T, Serafim T, Sardao V, Oliveira P. Analysis of pro-apoptotic protein trafficking to and from mitochondria. Methods Mol Biol. 2015;1241:163-80 pubmed publisher