This is a Validated Antibody Database (VAD) review about human Skp2, based on 43 published articles (read how Labome selects the articles), using Skp2 antibody in all methods. It is aimed to help Labome visitors find the most suited Skp2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Skp2 synonym: FBL1; FBXL1; FLB1; p45
Knockout validation
| Cell Signaling Technology domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (Cell Signaling, 2652) was used in western blot knockout validation on human samples at 1:1000 (fig 3i). Nat Commun (2018) ncbi |
Invitrogen
| mouse monoclonal (SKP2-8D9) |
| Invitrogen Skp2 antibody (eBioscience, 8D9) was used in flow cytometry on mouse samples (fig 5e). J Exp Med (2019) ncbi |
| mouse monoclonal (SKP2-8D9) |
| Invitrogen Skp2 antibody (eBioscience, 8D9) was used in flow cytometry on mouse samples (fig 8a). J Exp Med (2019) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to investigate activation of APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth by TGF-beta, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in western blot on human samples at 1:1000 (fig 1). Cell Cycle (2016) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the relationship between SKP2 and MYCN, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in western blot on human samples at 1:1000. Cancer Lett (2015) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the contribution of the Skp2-mH2A1-CDK8 axis to breast cancer, Invitrogen Skp2 antibody (Invitrogen, 323300) was used in western blot on human samples at 1:2000. Nat Commun (2015) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to identify the mechanistic link between Cullin neddylation and Myc ubiquitination/degradation, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in western blot on mouse samples . Nat Commun (2014) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the effect of imatinib on neuroblastoma cell lines, Invitrogen Skp2 antibody (Life Technologies, 32-3300) was used in western blot on human samples at 1:500-1:1000. Biochem Pharmacol (2014) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the involvement of RASSF1A inactivation in carcinogenesis, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples . Mol Cell Biol (2014) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to investigate the effect of epidermal growth factor on Skp2/Cks1 and p27kip1 in human extrahepatic cholangiocarcinoma cells, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in immunohistochemistry - paraffin section on human samples at 1:100 and in western blot on human samples at 1:1000 (fig 7). World J Gastroenterol (2014) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to discover a critical role for BRD4 in melanoma tumor maintenance, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in western blot on human samples (fig 6). Cancer Res (2013) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to elucidate the molecular mechanisms by which pVHL contributes to tumorigenesis, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). Oncogene (2011) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to test if small molecules can restore p27 expression in cancer cells, Invitrogen Skp2 antibody (Zymed Laboratories, 32-3300) was used in immunocytochemistry on human samples (fig 1c) and in western blot on human samples at 1:2000 (fig 1a). BMC Biol (2010) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to investigate IL-2-independent mechanisms of naive CD4 positive T cell proliferation, Invitrogen Skp2 antibody (Invitrogen, 32-3300) was used in western blot on human samples (fig 8). Immunology (2010) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to explore how the COP9 signalosome affects ubiquitination processes, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples (fig 2). J Biol Chem (2010) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study human papillomavirus type 18 E2 degradation, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples (fig 3). J Virol (2010) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to examine the role of Skp2 in the development of mechanical tension, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples (fig 3). Mol Cell Biol (2009) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to ascertain the effects of modulating Skp2 expression in melanoma cells, Invitrogen Skp2 antibody (Zymed, 8D9) was used in western blot on human samples (fig 3). Mol Biol Cell (2008) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to report that the Skp2-cyclin A interaction has a major role in cancer cell survival, Invitrogen Skp2 antibody (Zymed, 32-3,300) was used in flow cytometry on human samples (fig 1), in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Mol Cancer Ther (2007) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to examine how the COP9 signalosome regulates the cell cycle in human cells, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples . J Biol Chem (2006) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the role of B-RAF and cyclin D1 on p27Kip1 in human melanoma cells, Invitrogen Skp2 antibody (Zymed, Skp2-8D9) was used in western blot on human samples (fig 5). Oncogene (2007) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to determine the consequences of the Skp2-cyclin A interaction, Invitrogen Skp2 antibody (Zymed, 32-3300) was used in western blot on human samples (fig 2). J Biol Chem (2006) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the category subdivision of pT1a and pT1b in renal cell carcinoma, Invitrogen Skp2 antibody (Zymed, SKP2) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). BJU Int (2005) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to analyze the synergistic induction of apoptosis in T98G glioblastoma cells due to downregulation of Skp2 and p27/Kip1, Invitrogen Skp2 antibody (Zymed, 8D9) was used in western blot on human samples . J Mol Med (Berl) (2005) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to report that RhoA conveys the "cell shape signal" to the cell-cycle machinery in human capillary endothelial cells, Invitrogen Skp2 antibody (Zymed, SKP2-8D9) was used in western blot on human samples . J Biol Chem (2004) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study phosphorylation of human Cdt1 and induction of its degradation due to cyclin-dependent kinases, Invitrogen Skp2 antibody (Zymed, 8D9) was used in western blot on human samples (fig 2). J Biol Chem (2004) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to study the mechanisms of Cdt1 degradation in mammalian cells, Invitrogen Skp2 antibody (Zymed, 8D9) was used in western blot on human samples . J Biol Chem (2003) ncbi |
| mouse monoclonal (SKP2-8D9) |
| In order to assess the role of the SKP2 F-box protein in p27 regulation in prostate cancer, Invitrogen Skp2 antibody (Zymed, 32?C3300) was used in western blot on human samples at 1:250. BMC Cell Biol (2002) ncbi |
Santa Cruz Biotechnology
| mouse monoclonal (A-2) |
| Santa Cruz Biotechnology Skp2 antibody (SantaCruz, sc-74477) was used in western blot on human samples (fig 2e). Oncotarget (2017) ncbi |
Abcam
| domestic rabbit monoclonal (EPR3305(2)) |
| Abcam Skp2 antibody (Abcam, ab183039) was used in western blot on human samples (fig 2). Oncotarget (2016) ncbi |
Cell Signaling Technology
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (Cell Signaling, 4358) was used in immunoprecipitation on human samples (fig 2f) and in western blot on human samples (fig 5a). Pharmaceutics (2022) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (CST, 2652) was used in western blot on human samples at 1:1000 (fig 5h). Nat Commun (2021) ncbi |
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (Cell Signaling Technology, 4358) was used in western blot on human samples (fig 5d). Nat Commun (2020) ncbi |
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (Cell Signaling Technology, 4313) was used in western blot on mouse samples (fig 3b). Aging (Albany NY) (2020) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (Cell Signaling, 2652) was used in immunocytochemistry on human samples at 1:1000 (fig s4f) and in western blot on human samples at 1:1000 (fig s4d). Nat Commun (2018) ncbi |
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (New England Biolab, 4358) was used in western blot on human samples at 1:1000 (fig 5b). Oncogene (2018) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (Cell Signaling, 2652) was used in western blot knockout validation on human samples at 1:1000 (fig 3i). Nat Commun (2018) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (cell signalling, 2652) was used in immunohistochemistry on mouse samples (fig 7h), in immunoprecipitation on human samples (fig 3a), in immunohistochemistry on human samples at 1:1000 (fig 6b) and in western blot on human samples at 1:1000 (fig 1b). Nat Commun (2017) ncbi |
| domestic rabbit monoclonal (D3G5) |
| In order to identify and characterize an inhibitor of the COP9 signalosome, Cell Signaling Technology Skp2 antibody (Cell Signaling, 2652) was used in western blot on human samples at 1:1000 (fig 2c). Nat Commun (2016) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (Cell Signalling, 2652) was used in western blot on human samples (fig 2a). Oncotarget (2016) ncbi |
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (Cell Signaling Tech, 4358) was used in western blot on human samples (fig 6). Cancer Sci (2016) ncbi |
| domestic rabbit polyclonal |
| Cell Signaling Technology Skp2 antibody (Cell Signaling, 4313) was used in western blot on human samples (fig 2). Oncotarget (2016) ncbi |
| domestic rabbit monoclonal (D3G5) |
| In order to study MARCKS signaling and how it expresses endothelial cell proliferation and vascular smooth muscle through a KIS-, p27kip1- dependent mechanism, Cell Signaling Technology Skp2 antibody (Cell Signaling Technology, 2652) was used in western blot on human samples (fig 3b). PLoS ONE (2015) ncbi |
| domestic rabbit monoclonal (D3G5) |
| Cell Signaling Technology Skp2 antibody (Cell Signaling Technology, 2652) was used in western blot on human samples at 1:1000. FEBS J (2014) ncbi |
Articles Reviewed
- Ji P, Sun D, Wang H, Bauzon F, Zhu L. Disrupting Skp2-cyclin A interaction with a blocking peptide induces selective cancer cell killing. Mol Cancer Ther. 2007;6:684-91 pubmed
- Denti S, Fernandez Sanchez M, Rogge L, Bianchi E. The COP9 signalosome regulates Skp2 levels and proliferation of human cells. J Biol Chem. 2006;281:32188-96 pubmed
- Bhatt K, Hu R, Spofford L, Aplin A. Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells. Oncogene. 2007;26:1056-66 pubmed
- Ji P, Goldin L, Ren H, Sun D, Guardavaccaro D, Pagano M, et al. Skp2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27Kip1. J Biol Chem. 2006;281:24058-69 pubmed
- Langner C, Ratschek M, Rehak P, Tsybrovskyy O, Zigeuner R. The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?. BJU Int. 2005;95:310-4 pubmed
- Lee S, McCormick F. Downregulation of Skp2 and p27/Kip1 synergistically induces apoptosis in T98G glioblastoma cells. J Mol Med (Berl). 2005;83:296-307 pubmed
- Mammoto A, Huang S, Moore K, Oh P, Ingber D. Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition. J Biol Chem. 2004;279:26323-30 pubmed
- Liu E, Li X, Yan F, Zhao Q, Wu X. Cyclin-dependent kinases phosphorylate human Cdt1 and induce its degradation. J Biol Chem. 2004;279:17283-8 pubmed
- Li X, Zhao Q, Liao R, Sun P, Wu X. The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. J Biol Chem. 2003;278:30854-8 pubmed
- Lu L, Schulz H, Wolf D. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol. 2002;3:22 pubmed
articles
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