This is a Validated Antibody Database (VAD) review about human SYT1, based on 27 published articles (read how Labome selects the articles), using SYT1 antibody in all methods. It is aimed to help Labome visitors find the most suited SYT1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SYT1 synonym: BAGOS; P65; SVP65; SYT

Synaptic Systems
mouse monoclonal (41.1)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2f
  • western blot; mouse; loading ...; fig 2d
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in immunohistochemistry - frozen section on mouse samples (fig 2f) and in western blot on mouse samples (fig 2d). JCI Insight (2021) ncbi
mouse monoclonal (41.1)
  • immunohistochemistry; human; 1:500; loading ...; fig 1j
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in immunohistochemistry on human samples at 1:500 (fig 1j). Brain Commun (2021) ncbi
mouse monoclonal (41.1)
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1b
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in immunohistochemistry on mouse samples at 1:500 (fig 1b). elife (2020) ncbi
mouse monoclonal (41.1)
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1f
  • western blot; mouse; loading ...; fig s6b
Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011C5) was used in immunohistochemistry - frozen section on mouse samples (fig s1f) and in western blot on mouse samples (fig s6b). Neuron (2021) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; 1:1000; loading ...; fig 5b
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in western blot on mouse samples at 1:1000 (fig 5b). elife (2020) ncbi
mouse monoclonal (41.1)
  • immunocytochemistry; human; loading ...; fig s1e
Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011C2) was used in immunocytochemistry on human samples (fig s1e). Cell (2019) ncbi
mouse monoclonal (41.1)
  • immunohistochemistry - free floating section; mouse; 1:200; loading ...; fig 2d
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in immunohistochemistry - free floating section on mouse samples at 1:200 (fig 2d). J Neurosci (2017) ncbi
mouse monoclonal (41.1)
  • western blot; bovine; fig s1
Synaptic Systems SYT1 antibody (Synaptic Systems, 41.1) was used in western blot on bovine samples (fig s1). Sci Rep (2017) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; loading ...; tbl 1
In order to elucidate the contribution of the Sac phosphatase domain of Synaptojanin 1 to neurological symptoms, Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011) was used in western blot on mouse samples (tbl 1). Neuron (2017) ncbi
mouse monoclonal (41.1)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 2a
In order to characterize synaptic vesicle endocytosis, Synaptic Systems SYT1 antibody (SySy, 105 011) was used in immunocytochemistry on mouse samples at 1:100 (fig 2a). Neuron (2017) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; 1:5000; loading ...; fig 1a
In order to evaluate the application of CRISPR/Cas9 system in postmitotic cells, Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011) was used in western blot on mouse samples at 1:5000 (fig 1a). J Neurosci Methods (2017) ncbi
mouse monoclonal (41.1)
  • western blot; rat; 1:1000; loading ...; fig 5b
In order to develop methods to study vesicle-associated proteins and exocytosis in stellate astrocytes, Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in western blot on rat samples at 1:1000 (fig 5b). J Gen Physiol (2017) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; fig 2
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in western blot on mouse samples (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (41.1)
  • western blot; rat; loading ...; fig 6a
In order to investigate mechanisms involved in Rab35-mediated axonal elongation, Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011) was used in western blot on rat samples (fig 6a). J Neurosci (2016) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; 1:1000; loading ...; fig 3a
In order to investigate the function of extended synaptotagmins in knock out mice, Synaptic Systems SYT1 antibody (Synaptic systems, 41.1) was used in western blot on mouse samples at 1:1000 (fig 3a). PLoS ONE (2016) ncbi
mouse monoclonal (41.1)
  • immunohistochemistry; mouse; loading ...; fig 4a
  • western blot; mouse; loading ...; fig 2b
Synaptic Systems SYT1 antibody (Synaptic Systems, 105011) was used in immunohistochemistry on mouse samples (fig 4a) and in western blot on mouse samples (fig 2b). EBioMedicine (2016) ncbi
mouse monoclonal (41.1)
  • western blot; mouse; 1:300; fig 5
In order to characterize induced human neurons harboring alleles of four disease-associated single nucleotide polymorphisms in MIR137, Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011) was used in western blot on mouse samples at 1:300 (fig 5). Nat Neurosci (2015) ncbi
mouse monoclonal (41.1)
  • western blot; rat
In order to examine the biotinylation of vesicular transporters as a tool for the study of presynaptic function, Synaptic Systems SYT1 antibody (Synaptic Systems, 105 011) was used in western blot on rat samples . J Neurosci (2013) ncbi
Abcam
mouse monoclonal (ASV30)
  • western blot; mouse; loading ...; fig 4p
Abcam SYT1 antibody (Abcam, ab13259) was used in western blot on mouse samples (fig 4p). EBioMedicine (2022) ncbi
mouse monoclonal (ASV30)
  • immunocytochemistry; rat; loading ...
Abcam SYT1 antibody (Abcam, ab13259) was used in immunocytochemistry on rat samples . Sci Adv (2021) ncbi
mouse monoclonal (ASV30)
  • western blot; rat; 1:1000; loading ...; fig 2f
Abcam SYT1 antibody (Abcam, ab13259) was used in western blot on rat samples at 1:1000 (fig 2f). Aging Cell (2020) ncbi
mouse monoclonal (ASV30)
  • immunocytochemistry; mouse; 1:500; loading ...; fig 2b
Abcam SYT1 antibody (Abcam, ab13259) was used in immunocytochemistry on mouse samples at 1:500 (fig 2b). J Immunol Methods (2017) ncbi
mouse monoclonal (ASV30)
  • immunohistochemistry; rat; 1:100; fig 5
In order to utilize a system for neuromuscular junction development and maintenance to study mechanisms, Abcam SYT1 antibody (Abcam, ab13259) was used in immunohistochemistry on rat samples at 1:100 (fig 5). Development (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (41)
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology SYT1 antibody (Santa Cruz, sc-136088) was used in western blot on human samples (fig 2a). Toxicol Lett (2015) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (mAB 30 (asv30))
  • western blot; mouse; 1:50; loading ...; fig s6a
Developmental Studies Hybridoma Bank SYT1 antibody (DSHB, mAB30) was used in western blot on mouse samples at 1:50 (fig s6a). Proc Natl Acad Sci U S A (2018) ncbi
mouse monoclonal (mAB 30 (asv30))
  • immunohistochemistry; mouse; 1:100; fig 8
Developmental Studies Hybridoma Bank SYT1 antibody (DSHB, 30) was used in immunohistochemistry on mouse samples at 1:100 (fig 8). Nat Commun (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunocytochemistry; rat; loading ...; fig 3
In order to study the role of astrocytes to Alzheimer's disease, MilliporeSigma SYT1 antibody (Sigma, SAB4502907) was used in immunocytochemistry on rat samples (fig 3). J Alzheimers Dis (2016) ncbi
Articles Reviewed
  1. Zhou Q, Li S, Li M, Ke D, Wang Q, Yang Y, et al. Human tau accumulation promotes glycogen synthase kinase-3β acetylation and thus upregulates the kinase: A vicious cycle in Alzheimer neurodegeneration. EBioMedicine. 2022;78:103970 pubmed publisher
  2. Chen H, Zhang Brotzge X, Morozov Y, Li Y, Wang S, Zhang H, et al. Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis. JCI Insight. 2021;6: pubmed publisher
  3. Haytural H, Jordà Siquier T, Winblad B, Mulle C, Tjernberg L, Granholm A, et al. Distinctive alteration of presynaptic proteins in the outer molecular layer of the dentate gyrus in Alzheimer's disease. Brain Commun. 2021;3:fcab079 pubmed publisher
  4. Ivanova D, Dobson K, Gajbhiye A, Davenport E, Hacker D, Ultanir S, et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv. 2021;7: pubmed publisher
  5. Turecek J, Regehr W. Cerebellar and vestibular nuclear synapses in the inferior olive have distinct release kinetics and neurotransmitters. elife. 2020;9: pubmed publisher
  6. Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed publisher
  7. Sclip A, Sudhof T. LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses. elife. 2020;9: pubmed publisher
  8. Ye J, Yin Y, Liu H, Fang L, Tao X, Wei L, et al. Tau inhibits PKA by nuclear proteasome-dependent PKAR2α elevation with suppressed CREB/GluA1 phosphorylation. Aging Cell. 2020;19:e13055 pubmed publisher
  9. Patzke C, Brockmann M, Dai J, Gan K, Grauel M, Fenske P, et al. Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses. Cell. 2019;179:498-513.e22 pubmed publisher
  10. Wang Y, Figueiredo D, Sun X, Dong Z, Chen W, Cui W, et al. Controlling of glutamate release by neuregulin3 via inhibiting the assembly of the SNARE complex. Proc Natl Acad Sci U S A. 2018;115:2508-2513 pubmed publisher
  11. Yadirgi G, Stickings P, Rajagopal S, Liu Y, Sesardic D. Immuno-detection of cleaved SNAP-25 from differentiated mouse embryonic stem cells provides a sensitive assay for determination of botulinum A toxin and antitoxin potency. J Immunol Methods. 2017;451:90-99 pubmed publisher
  12. Bouhours B, Gjoni E, Kochubey O, Schneggenburger R. Synaptotagmin2 (Syt2) Drives Fast Release Redundantly with Syt1 at the Output Synapses of Parvalbumin-Expressing Inhibitory Neurons. J Neurosci. 2017;37:4604-4617 pubmed publisher
  13. Gümürdü A, Yildiz R, Eren E, Karakülah G, Unver T, Genc S, et al. MicroRNA exocytosis by large dense-core vesicle fusion. Sci Rep. 2017;7:45661 pubmed publisher
  14. Cao M, Wu Y, Ashrafi G, McCartney A, Wheeler H, Bushong E, et al. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron. 2017;93:882-896.e5 pubmed publisher
  15. Soykan T, Kaempf N, Sakaba T, Vollweiter D, Goerdeler F, Puchkov D, et al. Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly. Neuron. 2017;93:854-866.e4 pubmed publisher
  16. Horvath P, Kavalali E, Monteggia L. CRISPR/Cas9 system-mediated impairment of synaptobrevin/VAMP function in postmitotic hippocampal neurons. J Neurosci Methods. 2017;278:57-64 pubmed publisher
  17. Wolfes A, Ahmed S, Awasthi A, Stahlberg M, Rajput A, Magruder D, et al. A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes. J Gen Physiol. 2017;149:149-170 pubmed publisher
  18. Li Y, Chang L, Song Y, Gao X, Roselli F, Liu J, et al. Astrocytic GluN2A and GluN2B Oppose the Synaptotoxic Effects of Amyloid-?1-40 in Hippocampal Cells. J Alzheimers Dis. 2016;54:135-48 pubmed publisher
  19. Bodaleo F, Montenegro Venegas C, Henríquez D, Court F, Gonzalez Billault C. Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology. Sci Rep. 2016;6:30069 pubmed publisher
  20. Villarroel Campos D, Henríquez D, Bodaleo F, Oguchi M, Bronfman F, Fukuda M, et al. Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. J Neurosci. 2016;36:7298-313 pubmed publisher
  21. Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
  22. Emanuele M, Esposito A, Camerini S, Antonucci F, Ferrara S, Seghezza S, et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine. 2016;7:191-204 pubmed publisher
  23. Vilmont V, Cadot B, Ouanounou G, Gomes E. A system for studying mechanisms of neuromuscular junction development and maintenance. Development. 2016;143:2464-77 pubmed publisher
  24. Chiou Y, Liou S, Wong R, Chen C, Lee H. Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR-mutated lung cancer via nickel-induced microRNA-21 expression. Toxicol Lett. 2015;237:46-54 pubmed publisher
  25. Siegert S, Seo J, Kwon E, Rudenko A, Cho S, Wang W, et al. The schizophrenia risk gene product miR-137 alters presynaptic plasticity. Nat Neurosci. 2015;18:1008-16 pubmed publisher
  26. Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
  27. Xu W, Tse Y, Dobie F, Baudry M, Craig A, Wong T, et al. Simultaneous monitoring of presynaptic transmitter release and postsynaptic receptor trafficking reveals an enhancement of presynaptic activity in metabotropic glutamate receptor-mediated long-term depression. J Neurosci. 2013;33:5867-5877 pubmed publisher