This is a Validated Antibody Database (VAD) review about human SUPT6H, based on 5 published articles (read how Labome selects the articles), using SUPT6H antibody in all methods. It is aimed to help Labome visitors find the most suited SUPT6H antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SUPT6H synonym: SPT6; SPT6H; emb-5; transcription elongation factor SPT6; histone chaperone suppressor of Ty6; suppressor of Ty 6 homolog; tat-CT2 protein; tat-cotransactivator 2 protein

Abcam
rabbit polyclonal
  • immunocytochemistry; mouse; fig 6av
  • western blot; mouse; fig 4
In order to propose that a special mechanism of splicing occurs in the testis and brain whereby H2A.B.3 recruits RNA processing factors from splicing speckles, Abcam SUPT6H antibody (Abcam, ab32820) was used in immunocytochemistry on mouse samples (fig 6av) and in western blot on mouse samples (fig 4). PLoS Genet (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; African green monkey; 1:200; fig 5
Abcam SUPT6H antibody (Abcam, ab32820) was used in immunocytochemistry on African green monkey samples at 1:200 (fig 5). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; fig 4
In order to study the epigenetic mechanisms by which SUPT6H regulates estrogen receptor activity, Abcam SUPT6H antibody (Abcam, ab32820) was used in immunohistochemistry on human samples (fig 4). Oncogene (2015) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl SUPT6H antibody (Bethyl, A300-801A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2d
In order to learn about vulnerability in Ewing sarcoma by splicing of the EWS-FLI1 fusion transcript discovered thrugh functional genomic screening, Bethyl SUPT6H antibody (Bethyl Laboratories, A300- 801A) was used in western blot on human samples (fig 2d). Cell Rep (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 3, 5
In order to study the epigenetic mechanisms by which SUPT6H regulates estrogen receptor activity, Bethyl SUPT6H antibody (Bethyl, A300-802A) was used in immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig 3, 5). Oncogene (2015) ncbi
Articles Reviewed
  1. Soboleva T, Parker B, Nekrasov M, Hart Smith G, Tay Y, Tng W, et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet. 2017;13:e1006633 pubmed publisher
  2. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  3. Grohar P, Kim S, Rangel Rivera G, Sen N, Haddock S, Harlow M, et al. Functional Genomic Screening Reveals Splicing of the EWS-FLI1 Fusion Transcript as a Vulnerability in Ewing Sarcoma. Cell Rep. 2016;14:598-610 pubmed publisher
  4. Dembowski J, DeLuca N. Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes. PLoS Pathog. 2015;11:e1004939 pubmed publisher
  5. Bedi U, Scheel A, Hennion M, Begus Nahrmann Y, Ruschoff J, Johnsen S. SUPT6H controls estrogen receptor activity and cellular differentiation by multiple epigenomic mechanisms. Oncogene. 2015;34:465-73 pubmed publisher