This is a Validated Antibody Database (VAD) review about human SUPT16H, based on 4 published articles (read how Labome selects the articles), using SUPT16H antibody in all methods. It is aimed to help Labome visitors find the most suited SUPT16H antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SUPT16H synonym: CDC68; FACTP140; SPT16; SPT16/CDC68; FACT complex subunit SPT16; FACT 140 kDa subunit; chromatin-specific transcription elongation factor 140 kDa subunit; facilitates chromatin remodeling 140 kDa subunit; facilitates chromatin transcription complex subunit SPT16; hSPT16; suppressor of Ty 16 homolog

BioLegend
mouse monoclonal (8D2)
  • western blot; human; 1:1000; loading ...; fig 1a
In order to investigate the role of FACT in sensing DNA torsional stress, BioLegend SUPT16H antibody (BioLegend, 607002) was used in western blot on human samples at 1:1000 (fig 1a). Nucleic Acids Res (2017) ncbi
mouse monoclonal (8D2)
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 2
BioLegend SUPT16H antibody (Biolegend, 607001) was used in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 2). Epigenetics Chromatin (2016) ncbi
mouse monoclonal (8D2)
  • ChIP-Seq; human; fig 4
  • chromatin immunoprecipitation; human; fig 3
BioLegend SUPT16H antibody (BioLegend, 607002) was used in ChIP-Seq on human samples (fig 4) and in chromatin immunoprecipitation on human samples (fig 3). Mol Cell Biol (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D7I2K)
  • western blot; human; fig 4
In order to study the involvement of TIMELESS in the PARP1-mediated DNA damage response, Cell Signaling Technology SUPT16H antibody (Cell Signalling, 12191) was used in western blot on human samples (fig 4). Cell Rep (2015) ncbi
Articles Reviewed
  1. Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
  2. Sun J, Zhao Y, McGreal R, Cohen Tayar Y, Rockowitz S, Wilczek C, et al. Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers. Epigenetics Chromatin. 2016;9:37 pubmed publisher
  3. Young L, Marzio A, Pérez Durán P, Reid D, Meredith D, Roberti D, et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep. 2015;13:451-459 pubmed publisher
  4. Shakya A, Callister C, Goren A, Yosef N, Garg N, Khoddami V, et al. Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion. Mol Cell Biol. 2015;35:1014-25 pubmed publisher