This is a Validated Antibody Database (VAD) review about human SUN2, based on 14 published articles (read how Labome selects the articles), using SUN2 antibody in all methods. It is aimed to help Labome visitors find the most suited SUN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SUN2 synonym: UNC84B; SUN domain-containing protein 2; Sad1 unc-84 domain protein 2; nuclear envelope protein; protein unc-84 homolog B; rab5-interacting protein; rab5IP; sad1/unc-84 protein-like 2; unc-84 homolog B

Knockout validation
Abcam
rabbit monoclonal (EPR6557)
  • western blot knockout validation; mouse; 1:1000; fig s2
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 1
Abcam SUN2 antibody (Abcam, ab124916) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 1). J Cell Biol (2015) ncbi
Abcam
rabbit monoclonal (EPR6557)
  • western blot; human; 1:500; loading ...; fig s10c
Abcam SUN2 antibody (Abcam, ab124916) was used in western blot on human samples at 1:500 (fig s10c). Nature (2018) ncbi
rabbit monoclonal (EPR6557)
  • immunocytochemistry; human; 1:100; loading ...; fig s9a
In order to investigate the effect of actomyosin on cell nuclear morphology and genome stability, Abcam SUN2 antibody (Abcam, ab124916) was used in immunocytochemistry on human samples at 1:100 (fig s9a). Nat Commun (2017) ncbi
rabbit monoclonal (EPR6557)
  • western blot; hamsters; loading ...; fig s19a
In order to propose that local stresses applied to integrins propagate from the tensed actin cytoskeleton to the LINC complex and then through lamina-chromatin interactions to directly stretch chromatin and upregulate transcription, Abcam SUN2 antibody (Abcam, ab124916) was used in western blot on hamsters samples (fig s19a). Nat Mater (2016) ncbi
rabbit monoclonal (EPR6557)
  • western blot knockout validation; mouse; 1:1000; fig s2
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 1
Abcam SUN2 antibody (Abcam, ab124916) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 1). J Cell Biol (2015) ncbi
rabbit monoclonal (EPR6557)
  • immunocytochemistry; human
Abcam SUN2 antibody (Abcam, ab124916) was used in immunocytochemistry on human samples . Histochem Cell Biol (2014) ncbi
rabbit monoclonal (EPR6557)
  • western blot; human; loading ...; fig 2d
Abcam SUN2 antibody (Abcam, ab124916) was used in western blot on human samples (fig 2d). Sci Rep (2014) ncbi
ImmuQuest
mouse monoclonal ('LN43)
  • western blot; human; fig 1
ImmuQuest SUN2 antibody (Immuquest, LN43) was used in western blot on human samples (fig 1). Aging Cell (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; loading ...; fig s1
Sigma-Aldrich SUN2 antibody (Sigma, HPA001209) was used in western blot on human samples (fig s1). Methods (2019) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s3
Sigma-Aldrich SUN2 antibody (Sigma-Aldrich, HPA001209) was used in immunocytochemistry on human samples at 1:100 (fig s3). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:2000; fig 1
  • western blot; human; fig 2
Sigma-Aldrich SUN2 antibody (Sigma-Aldrich, HPA001209) was used in immunohistochemistry on human samples at 1:2000 (fig 1) and in western blot on human samples (fig 2). Sci Rep (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:300; fig s6
  • western blot; human; 1:2000; fig s6
Sigma-Aldrich SUN2 antibody (Sigma, HPA001209) was used in immunocytochemistry on human samples at 1:300 (fig s6) and in western blot on human samples at 1:2000 (fig s6). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500
In order to describe a method to investigate protein interactions in the nuclear envelope, Sigma-Aldrich SUN2 antibody (Atlas Antibodies, HPA001209) was used in western blot on human samples at 1:500. Biochim Biophys Acta (2014) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; mouse; fig 6a
EMD Millipore SUN2 antibody (Millipore, 06-1038) was used in immunocytochemistry on mouse samples (fig 6a). Methods Mol Biol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; fig 2
EMD Millipore SUN2 antibody (Millipore, 06-1038) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2). Cancer Med (2015) ncbi
Articles Reviewed
  1. Serebryannyy L, Misteli T. HiPLA: High-throughput imaging proximity ligation assay. Methods. 2019;157:80-87 pubmed publisher
  2. Frattini V, Pagnotta S, Tala -, Fan J, Russo M, Lee S, et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature. 2018;553:222-227 pubmed publisher
  3. Takaki T, Montagner M, Serres M, Le Berre M, Russell M, Collinson L, et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun. 2017;8:16013 pubmed publisher
  4. Tajik A, Zhang Y, Wei F, Sun J, Jia Q, Zhou W, et al. Transcription upregulation via force-induced direct stretching of chromatin. Nat Mater. 2016;15:1287-1296 pubmed publisher
  5. Korfali N, Florens L, Schirmer E. Isolation, Proteomic Analysis, and Microscopy Confirmation of the Liver Nuclear Envelope Proteome. Methods Mol Biol. 2016;1411:3-44 pubmed publisher
  6. Fahrenkrog B, Martinelli V, Nilles N, Fruhmann G, Chatel G, Juge S, et al. Expression of Leukemia-Associated Nup98 Fusion Proteins Generates an Aberrant Nuclear Envelope Phenotype. PLoS ONE. 2016;11:e0152321 pubmed publisher
  7. Lv X, Liu L, Cheng C, Yu B, Xiong L, Hu K, et al. SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer. Sci Rep. 2015;5:17940 pubmed publisher
  8. Chaumet A, Wright G, Seet S, Tham K, Gounko N, Bard F. Nuclear envelope-associated endosomes deliver surface proteins to the nucleus. Nat Commun. 2015;6:8218 pubmed publisher
  9. Matsumoto A, Hieda M, Yokoyama Y, Nishioka Y, Yoshidome K, Tsujimoto M, et al. Global loss of a nuclear lamina component, lamin A/C, and LINC complex components SUN1, SUN2, and nesprin-2 in breast cancer. Cancer Med. 2015;4:1547-57 pubmed publisher
  10. Stewart R, Zubek A, Rosowski K, Schreiner S, Horsley V, King M. Nuclear-cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. J Cell Biol. 2015;209:403-18 pubmed publisher
  11. Gibbs Seymour I, Markiewicz E, Bekker Jensen S, Mailand N, Hutchison C. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage. Aging Cell. 2015;14:162-9 pubmed publisher
  12. Kalendová A, Kalasová I, Yamazaki S, Uličná L, Harata M, Hozak P. Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. Histochem Cell Biol. 2014;142:139-52 pubmed publisher
  13. Jafferali M, Vijayaraghavan B, Figueroa R, Crafoord E, Gudise S, Larsson V, et al. MCLIP, an effective method to detect interactions of transmembrane proteins of the nuclear envelope in live cells. Biochim Biophys Acta. 2014;1838:2399-403 pubmed publisher
  14. Shaiken T, Opekun A. Dissecting the cell to nucleus, perinucleus and cytosol. Sci Rep. 2014;4:4923 pubmed publisher