This is a Validated Antibody Database (VAD) review about human STK4, based on 32 published articles (read how Labome selects the articles), using STK4 antibody in all methods. It is aimed to help Labome visitors find the most suited STK4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
STK4 synonym: KRS2; MST1; YSK3; serine/threonine-protein kinase 4; STE20-like kinase MST1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; serine/threonine-protein kinase Krs-2

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (RJ-5)
  • western blot knockout validation; human; loading ...; fig 1i
  • proximity ligation assay; human; 1:100; loading ...; fig 1a
Santa Cruz Biotechnology STK4 antibody (Santa Cruz, sc-100449) was used in western blot knockout validation on human samples (fig 1i) and in proximity ligation assay on human samples at 1:100 (fig 1a). J Vis Exp (2018) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 4b
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot knockout validation on human samples (fig 4b). J Biol Chem (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). PLoS ONE (2013) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 2
Cell Signaling Technology STK4 antibody (CST, 3682S) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2). Nat Cell Biol (2012) ncbi
Abcam
rabbit monoclonal (EP1465Y)
  • immunocytochemistry; mouse; loading ...; fig 4b
Abcam STK4 antibody (Abcam, ab51134) was used in immunocytochemistry on mouse samples (fig 4b). J Cell Mol Med (2019) ncbi
rabbit monoclonal (EP1465Y)
  • western blot; mouse; fig 3
Abcam STK4 antibody (Abcam, ab51134) was used in western blot on mouse samples (fig 3). J Cell Mol Med (2016) ncbi
rabbit monoclonal (EP1465Y)
  • immunohistochemistry; mouse; 1:100
  • western blot; mouse; 1:10000
Abcam STK4 antibody (Abcam, ab51134) was used in immunohistochemistry on mouse samples at 1:100 and in western blot on mouse samples at 1:10000. Cell Physiol Biochem (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (RJ-5)
  • western blot knockout validation; human; loading ...; fig 1i
  • proximity ligation assay; human; 1:100; loading ...; fig 1a
Santa Cruz Biotechnology STK4 antibody (Santa Cruz, sc-100449) was used in western blot knockout validation on human samples (fig 1i) and in proximity ligation assay on human samples at 1:100 (fig 1a). J Vis Exp (2018) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; loading ...; fig 5c
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 5i, 5a
Proteintech Group STK4 antibody (Proteintech, 22245-1-AP) was used in western blot on mouse samples (fig 5c), in immunoprecipitation on human samples (fig 5a) and in western blot on human samples (fig 5i, 5a). Cell Death Differ (2018) ncbi
Cell Signaling Technology
rabbit monoclonal (E7U1D)
  • western blot; human; 1:500; loading ...; fig 3a
Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 49332) was used in western blot on human samples at 1:500 (fig 3a). Cancer Manag Res (2019) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3a
Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 3682) was used in western blot on human samples at 1:1000 (fig 3a). Cancer Manag Res (2019) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 3b
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682S) was used in western blot on human samples at 1:500 (fig 3b). Oncotarget (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples (fig 2c). Cancer Res (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s3I
In order to find that that Fat4 modulates Hippo signaling and restricts hearth growth in mice, Cell Signaling Technology STK4 antibody (Cell signalling, 3682) was used in western blot on mouse samples at 1:1000 (fig s3I). Nat Commun (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 5d
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in immunoprecipitation on human samples (fig 5d). EMBO Rep (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6e
In order to find that CD4+ T cells from DOCK8-deficient mice produce large amounts of IL-31, Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on mouse samples at 1:1000 (fig 6e). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples (fig 4). Mol Clin Oncol (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig s2f
In order to demonstrate that the Hippo, Wnt/beta-catenin, and Notch pathways interact to regulate liver size and inhibit hepatocellular carcinoma, Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples (fig s2f). J Clin Invest (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 4
  • immunoprecipitation; mouse; fig 5
  • western blot; mouse; fig 4
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in immunoprecipitation on human samples (fig 5), in western blot on human samples (fig 4), in immunoprecipitation on mouse samples (fig 5) and in western blot on mouse samples (fig 4). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:2000; fig s3
In order to investigate the roles of NF2, LATS1/2, and YAP in the branching morphogenesis of the mouse kidney, Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on mouse samples at 1:2000 (fig s3). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 4b
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot knockout validation on human samples (fig 4b). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 3682) was used in western blot on human samples (fig 5). Chin J Cancer (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples (fig 4). Cell Signal (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples (fig 5). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig s3
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on mouse samples (fig s3). J Cell Biol (2015) ncbi
rabbit polyclonal
  • western blot; human; fig s2
In order to study the activation mechanisms of core Hippo pathway components, Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 3682) was used in western blot on human samples (fig s2). EMBO Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:2000; fig 1
Cell Signaling Technology STK4 antibody (Cell signaling, 3682) was used in western blot on human samples at 1:2000 (fig 1). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; dog; fig 5b
  • western blot; dog; 1:100; fig 3f
In order to assess the contribution of aPKC to epithelial cell transformation, Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 3682S) was used in immunocytochemistry on dog samples (fig 5b) and in western blot on dog samples at 1:100 (fig 3f). Mol Biol Cell (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on mouse samples at 1:1000 (fig 5). Nat Cell Biol (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig s2
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples at 1:1000 (fig s2). Nat Cell Biol (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig s10
  • western blot; human; fig 3
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in immunoprecipitation on human samples (fig s10) and in western blot on human samples (fig 3). Nat Commun (2014) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot on human samples . Biochimie (2014) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology STK4 antibody (Cell Signaling Technology, 3682) was used in western blot on human samples (fig 1a). Oncogene (2015) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Cell Signaling Technology STK4 antibody (Cell Signaling, 3682) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 2
Cell Signaling Technology STK4 antibody (CST, 3682S) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2). Nat Cell Biol (2012) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 1a
EMD Millipore STK4 antibody (Millipore, 07-061) was used in western blot on human samples (fig 1a). Biochim Biophys Acta Gene Regul Mech (2017) ncbi
Articles Reviewed
  1. Rong X, Han Q, Lin X, Kremerskothen J, Wang E. FRMPD1 activates the Hippo pathway via interaction with WWC3 to suppress the proliferation and invasiveness of lung cancer cells. Cancer Manag Res. 2019;11:3395-3410 pubmed publisher
  2. Karchugina S, Chernoff J. Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay. J Vis Exp. 2018;: pubmed publisher
  3. Cheng Z, Zhang M, Hu J, Lin J, Feng X, Wang S, et al. Cardiac-specific Mst1 deficiency inhibits ROS-mediated JNK signalling to alleviate Ang II-induced cardiomyocyte apoptosis. J Cell Mol Med. 2019;23:543-555 pubmed publisher
  4. Li W, Yue F, Dai Y, Shi B, Xu G, Jiang X, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ. 2018;: pubmed publisher
  5. Zheng X, Dong Q, Zhang X, Han Q, Han X, Han Y, et al. The coiled-coil domain of oncogene RASSF 7 inhibits hippo signaling and promotes non-small cell lung cancer. Oncotarget. 2017;8:78734-78748 pubmed publisher
  6. Toloczko A, Guo F, Yuen H, Wen Q, Wood S, Ong Y, et al. Deubiquitinating Enzyme USP9X Suppresses Tumor Growth via LATS Kinase and Core Components of the Hippo Pathway. Cancer Res. 2017;77:4921-4933 pubmed publisher
  7. Min K, Davila S, Zealy R, Lloyd L, Lee I, Lee R, et al. eIF4E phosphorylation by MST1 reduces translation of a subset of mRNAs, but increases lncRNA translation. Biochim Biophys Acta Gene Regul Mech. 2017;1860:761-772 pubmed publisher
  8. Ragni C, Diguet N, Le Garrec J, Novotova M, Resende T, Pop S, et al. Amotl1 mediates sequestration of the Hippo effector Yap1 downstream of Fat4 to restrict heart growth. Nat Commun. 2017;8:14582 pubmed publisher
  9. Heidary Arash E, Shiban A, Song S, Attisano L. MARK4 inhibits Hippo signaling to promote proliferation and migration of breast cancer cells. EMBO Rep. 2017;18:420-436 pubmed publisher
  10. Yamamura K, Uruno T, Shiraishi A, Tanaka Y, Ushijima M, Nakahara T, et al. The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. Nat Commun. 2017;8:13946 pubmed publisher
  11. Galoian K, Luo S, Qureshi A, Patel P, Price R, Morse A, et al. Effect of cytostatic proline rich polypeptide-1 on tumor suppressors of inflammation pathway signaling in chondrosarcoma. Mol Clin Oncol. 2016;5:618-624 pubmed
  12. Kim W, Khan S, Gvozdenovic Jeremic J, Kim Y, Dahlman J, Kim H, et al. Hippo signaling interactions with Wnt/?-catenin and Notch signaling repress liver tumorigenesis. J Clin Invest. 2017;127:137-152 pubmed publisher
  13. Vahid S, Thaper D, Gibson K, Bishop J, Zoubeidi A. Molecular chaperone Hsp27 regulates the Hippo tumor suppressor pathway in cancer. Sci Rep. 2016;6:31842 pubmed publisher
  14. Reginensi A, Enderle L, Gregorieff A, Johnson R, Wrana J, McNeill H. A critical role for NF2 and the Hippo pathway in branching morphogenesis. Nat Commun. 2016;7:12309 pubmed publisher
  15. Feng X, Liu P, Zhou X, Li M, Li F, Wang Z, et al. Thromboxane A2 Activates YAP/TAZ Protein to Induce Vascular Smooth Muscle Cell Proliferation and Migration. J Biol Chem. 2016;291:18947-58 pubmed publisher
  16. Wang D, Wu Y, Huang J, Wang W, Xu M, Jia J, et al. Hippo/YAP signaling pathway is involved in osteosarcoma chemoresistance. Chin J Cancer. 2016;35:47 pubmed publisher
  17. Hoa L, Kulaberoglu Y, Gundogdu R, Cook D, Mavis M, Gomez M, et al. The characterisation of LATS2 kinase regulation in Hippo-YAP signalling. Cell Signal. 2016;28:488-497 pubmed publisher
  18. Dai X, Zhuang L, Wang D, Zhou T, Chang L, Gai R, et al. Nuclear translocation and activation of YAP by hypoxia contributes to the chemoresistance of SN38 in hepatocellular carcinoma cells. Oncotarget. 2016;7:6933-47 pubmed publisher
  19. Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
  20. Giampietro C, Disanza A, Bravi L, Barrios Rodiles M, Corada M, Frittoli E, et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol. 2015;211:1177-92 pubmed publisher
  21. Kim M, Kim M, Park S, Lee C, Lim D. Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition. EMBO Rep. 2016;17:64-78 pubmed publisher
  22. Hu J, Man W, Shen M, Zhang M, Lin J, Wang T, et al. Luteolin alleviates post-infarction cardiac dysfunction by up-regulating autophagy through Mst1 inhibition. J Cell Mol Med. 2016;20:147-56 pubmed publisher
  23. Meng Z, Moroishi T, Mottier Pavie V, Plouffe S, Hansen C, Hong A, et al. MAP4K family kinases act in parallel to MST1/2 to activate LATS1/2 in the Hippo pathway. Nat Commun. 2015;6:8357 pubmed publisher
  24. Archibald A, Al Masri M, Liew Spilger A, McCaffrey L. Atypical protein kinase C induces cell transformation by disrupting Hippo/Yap signaling. Mol Biol Cell. 2015;26:3578-95 pubmed publisher
  25. Iglesias Bartolomé R, Torres D, Marone R, Feng X, Martin D, Simaan M, et al. Inactivation of a Gα(s)-PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis. Nat Cell Biol. 2015;17:793-803 pubmed publisher
  26. Mo J, Meng Z, Kim Y, Park H, Hansen C, Kim S, et al. Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway. Nat Cell Biol. 2015;17:500-10 pubmed publisher
  27. Xiang C, Li J, Hu L, Huang J, Luo T, Zhong Z, et al. Hippo signaling pathway reveals a spatio-temporal correlation with the size of primordial follicle pool in mice. Cell Physiol Biochem. 2015;35:957-68 pubmed publisher
  28. Kim M, Kim M, Lee M, Kim C, Lim D. The MST1/2-SAV1 complex of the Hippo pathway promotes ciliogenesis. Nat Commun. 2014;5:5370 pubmed publisher
  29. Chen S, Li D, Yang F, Wu Z, Zhao Y, Jiang Y. Gemcitabine-induced pancreatic cancer cell death is associated with MST1/cyclophilin D mitochondrial complexation. Biochimie. 2014;103:71-9 pubmed publisher
  30. Tanaka I, Osada H, Fujii M, Fukatsu A, Hida T, Horio Y, et al. LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade. Oncogene. 2015;34:73-83 pubmed publisher
  31. Li P, Chen Y, Mak K, Wong C, Wang C, Yuan P. Functional role of Mst1/Mst2 in embryonic stem cell differentiation. PLoS ONE. 2013;8:e79867 pubmed publisher
  32. Tumaneng K, Schlegelmilch K, Russell R, Yimlamai D, Basnet H, Mahadevan N, et al. YAP mediates crosstalk between the Hippo and PI(3)K–TOR pathways by suppressing PTEN via miR-29. Nat Cell Biol. 2012;14:1322-9 pubmed