This is a Validated Antibody Database (VAD) review about human STAG2, based on 6 published articles (read how Labome selects the articles), using STAG2 antibody in all methods. It is aimed to help Labome visitors find the most suited STAG2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
STAG2 synonym: MKMS; NEDXCF; SA-2; SA2; SCC3B; bA517O1.1

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig s1b
Cell Signaling Technology STAG2 antibody (Cell Signaling Technology, 4239) was used in western blot knockout validation on human samples (fig s1b). JCI Insight (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (J-12)
  • western blot; human; fig 4
Santa Cruz Biotechnology STAG2 antibody (Santa Cruz Biotechnology, J-12) was used in western blot on human samples (fig 4). PLoS Genet (2016) ncbi
mouse monoclonal (J-12)
  • western blot; human; fig 1d
Santa Cruz Biotechnology STAG2 antibody (Santa, sc-81852) was used in western blot on human samples (fig 1d). Nat Struct Mol Biol (2014) ncbi
mouse monoclonal (J-12)
  • immunohistochemistry; human; 1:100; fig 2
  • western blot; human; fig 5
In order to analyze the Ewing Sarcoma family of tumors for genomics to reveal STAG2 mutations, Santa Cruz Biotechnology STAG2 antibody (Santa Cruz, sc-81852) was used in immunohistochemistry on human samples at 1:100 (fig 2) and in western blot on human samples (fig 5). PLoS Genet (2014) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig s1b
Cell Signaling Technology STAG2 antibody (Cell Signaling Technology, 4239) was used in western blot knockout validation on human samples (fig s1b). JCI Insight (2021) ncbi
domestic rabbit monoclonal (D25A4)
  • RNA immunoprecipitation; human; fig 3
  • western blot; human; fig s3
Cell Signaling Technology STAG2 antibody (Cell Signaling, 5882S) was used in RNA immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig s3). Genome Biol (2016) ncbi
domestic rabbit monoclonal (D25A4)
  • western blot; human; 1:1000; loading ...; fig 6a
Cell Signaling Technology STAG2 antibody (Cell Signaling, 5882) was used in western blot on human samples at 1:1000 (fig 6a). EMBO J (2014) ncbi
Articles Reviewed
  1. Tothova Z, Valton A, Gorelov R, Vallurupalli M, Krill Burger J, Holmes A, et al. Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML. JCI Insight. 2021;6: pubmed publisher
  2. G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher
  3. Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher
  4. Sundaramoorthy S, Vázquez Novelle M, Lekomtsev S, Howell M, Petronczki M. Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion. EMBO J. 2014;33:2623-42 pubmed publisher
  5. Hara K, Zheng G, Qu Q, Liu H, Ouyang Z, Chen Z, et al. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nat Struct Mol Biol. 2014;21:864-70 pubmed publisher
  6. Brohl A, Solomon D, Chang W, Wang J, Song Y, Sindiri S, et al. The genomic landscape of the Ewing Sarcoma family of tumors reveals recurrent STAG2 mutation. PLoS Genet. 2014;10:e1004475 pubmed publisher