This is a Validated Antibody Database (VAD) review about human SSRP1, based on 11 published articles (read how Labome selects the articles), using SSRP1 antibody in all methods. It is aimed to help Labome visitors find the most suited SSRP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SSRP1 synonym: FACT; FACT80; T160

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (D-7)
  • western blot knockout validation; mouse; loading ...; fig 1e
Santa Cruz Biotechnology SSRP1 antibody (Santa Cruz, sc-74536) was used in western blot knockout validation on mouse samples (fig 1e). Nucleic Acids Res (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-7)
  • western blot knockout validation; mouse; loading ...; fig 1e
Santa Cruz Biotechnology SSRP1 antibody (Santa Cruz, sc-74536) was used in western blot knockout validation on mouse samples (fig 1e). Nucleic Acids Res (2020) ncbi
mouse monoclonal (10D7)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology SSRP1 antibody (SantaCruz, sc-56781) was used in western blot on human samples (fig 3a). Open Biol (2016) ncbi
mouse monoclonal (3E4)
  • immunocytochemistry; human; fig 1
  • western blot; human; 1:500; fig 1
Santa Cruz Biotechnology SSRP1 antibody (Santa Cruz, sc-56,782) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples at 1:500 (fig 1). J Mol Biol (2016) ncbi
mouse monoclonal (D-7)
  • western blot; human; 1:1000; fig 3
Santa Cruz Biotechnology SSRP1 antibody (Santa Cruz, sc74536) was used in western blot on human samples at 1:1000 (fig 3). J Mol Biol (2015) ncbi
Abcam
domestic rabbit monoclonal (EPR7895)
  • ChIP-Seq; mouse; loading ...; fig 3h
  • chromatin immunoprecipitation; mouse; loading ...; fig 3i
Abcam SSRP1 antibody (Abcam, ab137034) was used in ChIP-Seq on mouse samples (fig 3h) and in chromatin immunoprecipitation on mouse samples (fig 3i). Nucleic Acids Res (2020) ncbi
mouse monoclonal (10D7)
  • western blot; human; fig 4
In order to study the involvement of TIMELESS in the PARP1-mediated DNA damage response, Abcam SSRP1 antibody (Abcam, ab26212) was used in western blot on human samples (fig 4). Cell Rep (2015) ncbi
BioLegend
mouse monoclonal (10D1)
  • EMSA; human; fig 7a
  • immunocytochemistry; human; 1:200; loading ...; fig 1b
  • western blot; human; 1:4000; loading ...; fig 1a
In order to investigate the role of FACT in sensing DNA torsional stress, BioLegend SSRP1 antibody (BioLegend, 609702) was used in EMSA on human samples (fig 7a), in immunocytochemistry on human samples at 1:200 (fig 1b) and in western blot on human samples at 1:4000 (fig 1a). Nucleic Acids Res (2017) ncbi
mouse monoclonal (10D1)
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 2
BioLegend SSRP1 antibody (Biolegend, 609702) was used in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 2). Epigenetics Chromatin (2016) ncbi
mouse monoclonal (10D1)
  • immunocytochemistry; African green monkey; 1:200; fig 5
BioLegend SSRP1 antibody (Biolegend, 10D1) was used in immunocytochemistry on African green monkey samples at 1:200 (fig 5). PLoS Pathog (2015) ncbi
mouse monoclonal (10D1)
  • chromatin immunoprecipitation; human; fig 3
BioLegend SSRP1 antibody (BioLegend, 609702) was used in chromatin immunoprecipitation on human samples (fig 3). Mol Cell Biol (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (E1Y8D)
  • western blot; human; fig 5f
Cell Signaling Technology SSRP1 antibody (Cell Signaling, 13421) was used in western blot on human samples (fig 5f). Mol Cell (2018) ncbi
domestic rabbit monoclonal (E1Y8D)
  • western blot; fruit fly ; 1:5000
In order to describe a SUMO-specific protease-based methodology suitable for mass spectroscopy, Cell Signaling Technology SSRP1 antibody (Cell Signaling Technology, 13421S) was used in western blot on fruit fly samples at 1:5000. Nat Commun (2015) ncbi
Articles Reviewed
  1. Chen F, Zhang W, Xie D, Gao T, Dong Z, Lu X. Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters. Nucleic Acids Res. 2020;48:10211-10225 pubmed publisher
  2. Kim J, Sturgill D, Sebastian R, Khurana S, Tran A, Edwards G, et al. Replication Stress Shapes a Protective Chromatin Environment across Fragile Genomic Regions. Mol Cell. 2018;69:36-47.e7 pubmed publisher
  3. Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
  4. Samson A, Ho B, Au A, Schoenwaelder S, Smyth M, Bottomley S, et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol. 2016;6: pubmed
  5. Sun J, Zhao Y, McGreal R, Cohen Tayar Y, Rockowitz S, Wilczek C, et al. Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers. Epigenetics Chromatin. 2016;9:37 pubmed publisher
  6. Lopez A, Kugelman J, Garcia Rivera J, Urias E, Salinas S, Fernandez Zapico M, et al. The Structure-Specific Recognition Protein 1 Associates with Lens Epithelium-Derived Growth Factor Proteins and Modulates HIV-1 Replication. J Mol Biol. 2016;428:2814-31 pubmed publisher
  7. Young L, Marzio A, Pérez Durán P, Reid D, Meredith D, Roberti D, et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep. 2015;13:451-459 pubmed publisher
  8. Hendriks I, D Souza R, Chang J, Mann M, Vertegaal A. System-wide identification of wild-type SUMO-2 conjugation sites. Nat Commun. 2015;6:7289 pubmed publisher
  9. Dembowski J, DeLuca N. Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes. PLoS Pathog. 2015;11:e1004939 pubmed publisher
  10. Szerlong H, Herman J, Krause C, DeLuca J, Skoultchi A, Winger Q, et al. Proteomic characterization of the nucleolar linker histone H1 interaction network. J Mol Biol. 2015;427:2056-71 pubmed publisher
  11. Shakya A, Callister C, Goren A, Yosef N, Garg N, Khoddami V, et al. Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion. Mol Cell Biol. 2015;35:1014-25 pubmed publisher