This is a Validated Antibody Database (VAD) review about human SRp20, based on 31 published articles (read how Labome selects the articles), using SRp20 antibody in all methods. It is aimed to help Labome visitors find the most suited SRp20 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SRp20 synonym: SFRS3; SRp20

Knockout validation
ATCC
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 3e, s3b
  • western blot; human; 1:1000; loading ...; fig 4c, 8e
ATCC SRp20 antibody (ATCC, CRL-2384) was used in western blot knockout validation on mouse samples at 1:1000 (fig 3e, s3b) and in western blot on human samples at 1:1000 (fig 4c, 8e). Adv Sci (Weinh) (2022) ncbi
Invitrogen
mouse monoclonal (7B4 (7B4A12))
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
  • western blot; human; 1:250; fig 2
In order to assess regulation of SRSF3 (SRp20) to promote capsid protein expression in infected differentiated keratinocytes by human papillomavirus E2, Invitrogen SRp20 antibody (Invitrogen, 33-4200) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3) and in western blot on human samples at 1:250 (fig 2). J Virol (2016) ncbi
mouse monoclonal (7B4 (7B4A12))
  • immunohistochemistry - paraffin section; human; 1:300; loading ...; tbl s2
In order to explore the relationship between hnRNP A1 and RON tumoral expression, Invitrogen SRp20 antibody (Invitrogen, 7B4A12) was used in immunohistochemistry - paraffin section on human samples at 1:300 (tbl s2). Oncotarget (2016) ncbi
mouse monoclonal (7B4 (7B4A12))
  • western blot; human; fig 1
In order to study the role between SRSF3 and how it represses the expression of PDCD4 protein by coordinated regulation and alternative splicing, translation and export, Invitrogen SRp20 antibody (Invitrogen, 33-4200) was used in western blot on human samples (fig 1). Biochem Biophys Res Commun (2016) ncbi
mouse monoclonal (7B4 (7B4A12))
  • cross-linking immunoprecipitation; human; fig 4
In order to assess JNK-dependent alternative splicing in T-cell activation through CELF2-mediated regulation of MKK7, Invitrogen SRp20 antibody (Abcam, 33-4200) was used in cross-linking immunoprecipitation on human samples (fig 4). Genes Dev (2015) ncbi
mouse monoclonal (1H4G7)
  • western blot; human
In order to investigate viral tat-mRNA splicing, Invitrogen SRp20 antibody (Invitrogen, 1H4G7) was used in western blot on human samples . Retrovirology (2015) ncbi
mouse monoclonal (16H3 (16H3E8))
  • immunocytochemistry; human; 1:200; fig 3
In order to examine the methylation state of ATXN2L and its contribution to ATXN2L localization, Invitrogen SRp20 antibody (Life Technologies, 16H3) was used in immunocytochemistry on human samples at 1:200 (fig 3). Exp Cell Res (2015) ncbi
mouse monoclonal (7B4 (7B4A12))
  • immunohistochemistry; rat; 1:500; fig 6
In order to elucidate the mechanisms that regulate RGS4 expression, Invitrogen SRp20 antibody (Invitrogen, 33-4200) was used in immunohistochemistry on rat samples at 1:500 (fig 6). PLoS ONE (2013) ncbi
mouse monoclonal (7B4 (7B4A12))
  • immunocytochemistry; human; fig 5
In order to elucidate the mechanisms of action of two small molecule modulators of HIV-1 RNA processing, Invitrogen SRp20 antibody (Invitrogen, 334200) was used in immunocytochemistry on human samples (fig 5). Nucleic Acids Res (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to report that Thoc5 and CFIm68 interact, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). Nucleic Acids Res (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to explore the mechanism underlying paraquat-induced pre-mRNA splicing alterations, Invitrogen SRp20 antibody (Invitrogen, clone 16H3) was used in western blot on human samples (fig 1). PLoS ONE (2013) ncbi
mouse monoclonal (1H4G7)
  • western blot; human; 1:5000; fig 6
In order to examine the effects of RNA splicing modulators on viral RNA metabolism, Invitrogen SRp20 antibody (Invitrogen, 33-9400) was used in western blot on human samples at 1:5000 (fig 6). PLoS Pathog (2013) ncbi
mouse monoclonal (1H4G7)
  • chromatin immunoprecipitation; mouse; fig 1
  • western blot; mouse; fig 1
In order to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA, Invitrogen SRp20 antibody (Invitrogen, clone 1H4) was used in chromatin immunoprecipitation on mouse samples (fig 1) and in western blot on mouse samples (fig 1). J Biol Chem (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; rat; fig 3
In order to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA, Invitrogen SRp20 antibody (Invitrogen, clone 16H3) was used in western blot on rat samples (fig 3). J Biol Chem (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:100; fig 3
  • western blot; mouse; 1:100; fig 3
In order to assess the splicing machinery in Down syndrome brains, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples at 1:100 (fig 3) and in western blot on mouse samples at 1:100 (fig 3). Neurobiol Dis (2010) ncbi
mouse monoclonal (1H4G7)
  • immunohistochemistry; mouse; 1:100; fig 3
  • western blot; mouse; 1:100; fig 3
  • immunohistochemistry; human; 1:100; fig 3
  • western blot; human; 1:100; fig 3
In order to assess the splicing machinery in Down syndrome brains, Invitrogen SRp20 antibody (Zymed, 1H4) was used in immunohistochemistry on mouse samples at 1:100 (fig 3), in western blot on mouse samples at 1:100 (fig 3), in immunohistochemistry on human samples at 1:100 (fig 3) and in western blot on human samples at 1:100 (fig 3). Neurobiol Dis (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to identify factors that modulate TF pre-mRNA splicing, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). J Leukoc Biol (2010) ncbi
mouse monoclonal (1H4G7)
  • western blot; hamsters
In order to study how stress-regulated miRs regulate alternative splicing, Invitrogen SRp20 antibody (Zymed, 339400) was used in western blot on hamsters samples . J Mol Neurosci (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 3
In order to examine the effect of extracellular pH on SMN2 exon 7 splicing in spinal muscular atrophy cells, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples (fig 3). Mol Cell Neurosci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:2000; fig 1
  • western blot; mouse; fig 2
In order to assess how SR kinases play a role in alternative splicing through dynamic balance of phosphorylation, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples at 1:2000 (fig 1) and in western blot on mouse samples (fig 2). Genes Cells (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • immunoprecipitation; human; fig 4
In order to determine whether mutations within 5' splice site downstream of exon2 result in reduced or increased HIV-1 Vif expression, Invitrogen SRp20 antibody (Zymed, 16H3) was used in immunoprecipitation on human samples (fig 4). J Virol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 2
In order to determine the effect of hydroxyurea in spinal muscular atrophy cells and patients, Invitrogen SRp20 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples (fig 2). J Neurol Sci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:500
In order to investigate if inhibitors of the Na+/H+ exchanger modulate the exon 7 splicing of survival motor neuron 2 mRNA in spinal muscular atrophy patients, Invitrogen SRp20 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples at 1:500. Ann Neurol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:1000
In order to determine the molecular mechanisms associated with ankyloblepharon, ectodermal dysplasia, and clefting, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on human samples at 1:1000. Cell Cycle (2005) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; mouse
In order to examine the therapeutic use of sodium butyrate to treat spinal muscular atrophy, Invitrogen SRp20 antibody (Zymed, 16H3) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2001) ncbi
Santa Cruz Biotechnology
mouse monoclonal (7B4)
  • western blot; human; loading ...; fig 5c
Santa Cruz Biotechnology SRp20 antibody (Santa Cruz, sc-13510) was used in western blot on human samples (fig 5c). Cell Death Dis (2021) ncbi
mouse monoclonal (3G271)
  • western blot; human; loading ...; fig 3e
Santa Cruz Biotechnology SRp20 antibody (Santa Cruz Biotechnology, 3G271) was used in western blot on human samples (fig 3e). Biomed Res Int (2019) ncbi
mouse monoclonal (1H4)
  • western blot; human; 1:200; fig 1
In order to investigate the impact of serine-arginine rich splicing factor 2 mutations to myelodysplastic syndrome, Santa Cruz Biotechnology SRp20 antibody (Santa Cruz, sc-13,509) was used in western blot on human samples at 1:200 (fig 1). BMC Mol Biol (2016) ncbi
mouse monoclonal (7B4)
  • western blot; human
Santa Cruz Biotechnology SRp20 antibody (Santa Cruz Biotechnology, sc-13510) was used in western blot on human samples . Mol Cancer (2015) ncbi
Abcam
domestic rabbit monoclonal (EPR16976)
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam SRp20 antibody (Abcam, ab198291) was used in western blot on human samples at 1:1000 (fig 1a). Aging (Albany NY) (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; fig s3
In order to investigate MDM4 exon 6 skipping by antisense oligionucleotides and its affect on inhibiting tumor growth, Abcam SRp20 antibody (Abcam, ab125124) was used in western blot on human samples at 1:500 (fig s3). J Clin Invest (2016) ncbi
ATCC
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 3e, s3b
  • western blot; human; 1:1000; loading ...; fig 4c, 8e
ATCC SRp20 antibody (ATCC, CRL-2384) was used in western blot knockout validation on mouse samples at 1:1000 (fig 3e, s3b) and in western blot on human samples at 1:1000 (fig 4c, 8e). Adv Sci (Weinh) (2022) ncbi
MBL International
polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
  • western blot; human; 1:1000; fig 1
In order to compare colorectal and gastric cancer and their expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1, MBL International SRp20 antibody (MBL, RN080PW) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1) and in western blot on human samples at 1:1000 (fig 1). BMC Cancer (2016) ncbi
polyclonal
In order to develop reagents to study RNA binding proteins, MBL International SRp20 antibody (MBLI, RN080PW) was used . Mol Cell (2016) ncbi
Articles Reviewed
  1. Kumar D, Das M, Oberg A, Sahoo D, Wu P, Sauceda C, et al. Hepatocyte Deletion of IGF2 Prevents DNA Damage and Tumor Formation in Hepatocellular Carcinoma. Adv Sci (Weinh). 2022;9:e2105120 pubmed publisher
  2. Chen D, Zhao Z, Chen L, Li Q, Zou J, Liu S. PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3. Cell Death Dis. 2021;12:722 pubmed publisher
  3. Xu L, Shen J, Jia J, Jia R. Inclusion of hnRNP L Alternative Exon 7 Is Associated with Good Prognosis and Inhibited by Oncogene SRSF3 in Head and Neck Squamous Cell Carcinoma. Biomed Res Int. 2019;2019:9612425 pubmed publisher
  4. Shen T, Li H, Song Y, Li L, Lin J, Wei G, et al. Alternative polyadenylation dependent function of splicing factor SRSF3 contributes to cellular senescence. Aging (Albany NY). 2019;11:1356-1388 pubmed publisher
  5. Skrdlant L, Stark J, Lin R. Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C. BMC Mol Biol. 2016;17:18 pubmed publisher
  6. Park W, Kim H, Kang D, Ryu J, Choi K, Lee G, et al. Comparative expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1 in gastric and colorectal cancer. BMC Cancer. 2016;16:358 pubmed publisher
  7. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  8. Klymenko T, Hernández López H, MacDonald A, Bodily J, Graham S. Human Papillomavirus E2 Regulates SRSF3 (SRp20) To Promote Capsid Protein Expression in Infected Differentiated Keratinocytes. J Virol. 2016;90:5047-58 pubmed publisher
  9. Cammas A, Lacroix Triki M, Pierredon S, Le Bras M, Iacovoni J, Teulade Fichou M, et al. hnRNP A1-mediated translational regulation of the G quadruplex-containing RON receptor tyrosine kinase mRNA linked to tumor progression. Oncotarget. 2016;7:16793-805 pubmed publisher
  10. Park S, Jeong S. SRSF3 represses the expression of PDCD4 protein by coordinated regulation of alternative splicing, export and translation. Biochem Biophys Res Commun. 2016;470:431-438 pubmed publisher
  11. Dewaele M, Tabaglio T, Willekens K, Bezzi M, Teo S, Low D, et al. Antisense oligonucleotide-mediated MDM4 exon 6 skipping impairs tumor growth. J Clin Invest. 2016;126:68-84 pubmed publisher
  12. Martinez N, Agosto L, Qiu J, Mallory M, Gazzara M, Barash Y, et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev. 2015;29:2054-66 pubmed publisher
  13. He X, Zhang P. Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair. Mol Cancer. 2015;14:158 pubmed publisher
  14. Erkelenz S, Hillebrand F, Widera M, Theiss S, Fayyaz A, Degrandi D, et al. Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication. Retrovirology. 2015;12:29 pubmed publisher
  15. Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
  16. Li S, Li Y, Cui S, Qi Y, Zhao J, Liu X, et al. Splicing factor transformer-2? (Tra2?) regulates the expression of regulator of G protein signaling 4 (RGS4) gene and is induced by morphine. PLoS ONE. 2013;8:e72220 pubmed publisher
  17. Wong R, Balachandran A, Haaland M, Stoilov P, Cochrane A. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res. 2013;41:9471-83 pubmed publisher
  18. Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res. 2013;41:7060-72 pubmed publisher
  19. Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
  20. Wong R, Balachandran A, Ostrowski M, Cochrane A. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog. 2013;9:e1003241 pubmed publisher
  21. Walsh C, Suchanek A, Cyphert T, Kohan A, Szeszel Fedorowicz W, Salati L. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver. J Biol Chem. 2013;288:2816-28 pubmed publisher
  22. Toiber D, Azkona G, Ben Ari S, Toran N, Soreq H, Dierssen M. Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations. Neurobiol Dis. 2010;40:348-59 pubmed publisher
  23. Chandradas S, Deikus G, Tardos J, Bogdanov V. Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 2010;87:147-52 pubmed publisher
  24. Meerson A, Cacheaux L, Goosens K, Sapolsky R, Soreq H, Kaufer D. Changes in brain MicroRNAs contribute to cholinergic stress reactions. J Mol Neurosci. 2010;40:47-55 pubmed publisher
  25. Chen Y, Yuo C, Yang W, Jong Y, Lin H, Chang Y, et al. Extracellular pH change modulates the exon 7 splicing in SMN2 mRNA. Mol Cell Neurosci. 2008;39:268-72 pubmed publisher
  26. Yomoda J, Muraki M, Kataoka N, Hosoya T, Suzuki M, Hagiwara M, et al. Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. Genes Cells. 2008;13:233-44 pubmed publisher
  27. Exline C, Feng Z, Stoltzfus C. Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. J Virol. 2008;82:3921-31 pubmed publisher
  28. Liang W, Yuo C, Chang J, Chen Y, Chang Y, Wang H, et al. The effect of hydroxyurea in spinal muscular atrophy cells and patients. J Neurol Sci. 2008;268:87-94 pubmed publisher
  29. Yuo C, Lin H, Chang Y, Yang W, Chang J. 5-(N-ethyl-N-isopropyl)-amiloride enhances SMN2 exon 7 inclusion and protein expression in spinal muscular atrophy cells. Ann Neurol. 2008;63:26-34 pubmed
  30. Huang Y, Kim Y, Li Z, Fomenkov T, Fomenkov A, Ratovitski E. AEC-associated p63 mutations lead to alternative splicing/protein stabilization of p63 and modulation of Notch signaling. Cell Cycle. 2005;4:1440-7 pubmed
  31. Chang J, Hsieh Li H, Jong Y, Wang N, Tsai C, Li H. Treatment of spinal muscular atrophy by sodium butyrate. Proc Natl Acad Sci U S A. 2001;98:9808-13 pubmed