This is a Validated Antibody Database (VAD) review about human SRSF6, based on 21 published articles (read how Labome selects the articles), using SRSF6 antibody in all methods. It is aimed to help Labome visitors find the most suited SRSF6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SRSF6 synonym: B52; HEL-S-91; SFRS6; SRP55

Invitrogen
mouse monoclonal (16H3 (16H3E8))
  • immunocytochemistry; human; 1:200; fig 3
In order to examine the methylation state of ATXN2L and its contribution to ATXN2L localization, Invitrogen SRSF6 antibody (Life Technologies, 16H3) was used in immunocytochemistry on human samples at 1:200 (fig 3). Exp Cell Res (2015) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to report that Thoc5 and CFIm68 interact, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). Nucleic Acids Res (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to explore the mechanism underlying paraquat-induced pre-mRNA splicing alterations, Invitrogen SRSF6 antibody (Invitrogen, clone 16H3) was used in western blot on human samples (fig 1). PLoS ONE (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; rat; fig 3
In order to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA, Invitrogen SRSF6 antibody (Invitrogen, clone 16H3) was used in western blot on rat samples (fig 3). J Biol Chem (2013) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:100; fig 3
  • western blot; mouse; 1:100; fig 3
In order to assess the splicing machinery in Down syndrome brains, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on human samples at 1:100 (fig 3) and in western blot on mouse samples at 1:100 (fig 3). Neurobiol Dis (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 1
In order to identify factors that modulate TF pre-mRNA splicing, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on human samples (fig 1). J Leukoc Biol (2010) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 3
In order to examine the effect of extracellular pH on SMN2 exon 7 splicing in spinal muscular atrophy cells, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on human samples (fig 3). Mol Cell Neurosci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; mouse; fig 2
  • western blot; human; 1:2000; fig 1
In order to assess how SR kinases play a role in alternative splicing through dynamic balance of phosphorylation, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on mouse samples (fig 2) and in western blot on human samples at 1:2000 (fig 1). Genes Cells (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • immunoprecipitation; human; fig 4
In order to determine whether mutations within 5' splice site downstream of exon2 result in reduced or increased HIV-1 Vif expression, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in immunoprecipitation on human samples (fig 4). J Virol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; fig 2
In order to determine the effect of hydroxyurea in spinal muscular atrophy cells and patients, Invitrogen SRSF6 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples (fig 2). J Neurol Sci (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:500
In order to investigate if inhibitors of the Na+/H+ exchanger modulate the exon 7 splicing of survival motor neuron 2 mRNA in spinal muscular atrophy patients, Invitrogen SRSF6 antibody (Zymed Laboratories, 16H3) was used in western blot on human samples at 1:500. Ann Neurol (2008) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; human; 1:1000
In order to determine the molecular mechanisms associated with ankyloblepharon, ectodermal dysplasia, and clefting, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on human samples at 1:1000. Cell Cycle (2005) ncbi
mouse monoclonal (16H3 (16H3E8))
  • western blot; mouse
In order to examine the therapeutic use of sodium butyrate to treat spinal muscular atrophy, Invitrogen SRSF6 antibody (Zymed, 16H3) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2001) ncbi
Abcam
domestic rabbit monoclonal (EPR9231)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 8b
  • western blot; human; 1:1000; loading ...; fig 3e
Abcam SRSF6 antibody (Abcam, ab140623) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 8b) and in western blot on human samples at 1:1000 (fig 3e). Am J Cancer Res (2020) ncbi
domestic rabbit monoclonal (EPR9231)
  • western blot; human; 1:1000; loading ...; fig 3c
  • western blot; mouse; 1:1000; loading ...; fig 3c
Abcam SRSF6 antibody (Abcam, ab140623) was used in western blot on human samples at 1:1000 (fig 3c) and in western blot on mouse samples at 1:1000 (fig 3c). Nat Commun (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1H4)
  • western blot; human; 1:200; fig 1
In order to investigate the impact of serine-arginine rich splicing factor 2 mutations to myelodysplastic syndrome, Santa Cruz Biotechnology SRSF6 antibody (Santa Cruz, sc-13,509) was used in western blot on human samples at 1:200 (fig 1). BMC Mol Biol (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
  • western blot; human; 1:1000; fig 1
In order to compare colorectal and gastric cancer and their expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1, MilliporeSigma SRSF6 antibody (Sigma-Aldrich, HPA029005) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1) and in western blot on human samples at 1:1000 (fig 1). BMC Cancer (2016) ncbi
EMD Millipore
mouse monoclonal (16H3)
  • western blot; human; loading ...; fig s5a
EMD Millipore SRSF6 antibody (Millipore, MABE126) was used in western blot on human samples (fig s5a). Nat Commun (2017) ncbi
mouse monoclonal (9-1-56)
  • western blot; human; fig 1
In order to study alteration of MAP2 splicing in Huntington's disease, EMD Millipore SRSF6 antibody (Merck Millipore, MABE152) was used in western blot on human samples (fig 1). Brain Pathol (2017) ncbi
mouse monoclonal (16H3)
  • western blot; human; 1:1000
EMD Millipore SRSF6 antibody (Merck Millipore, MABE126) was used in western blot on human samples at 1:1000. Br J Pharmacol (2015) ncbi
mouse monoclonal (9-1-56)
  • immunoprecipitation; human
  • western blot; human
EMD Millipore SRSF6 antibody (Millipore, MABE152) was used in immunoprecipitation on human samples and in western blot on human samples . Mol Cell Biol (2015) ncbi
Articles Reviewed
  1. Li M, Wu P, Yang Z, Deng S, Ni L, Zhang Y, et al. miR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1. Am J Cancer Res. 2020;10:38-59 pubmed
  2. Neueder A, Dumas A, Benjamin A, Bates G. Regulatory mechanisms of incomplete huntingtin mRNA splicing. Nat Commun. 2018;9:3955 pubmed publisher
  3. Funnell T, Tasaki S, Oloumi A, Araki S, Kong E, Yap D, et al. CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor. Nat Commun. 2017;8:7 pubmed publisher
  4. Skrdlant L, Stark J, Lin R. Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C. BMC Mol Biol. 2016;17:18 pubmed publisher
  5. Park W, Kim H, Kang D, Ryu J, Choi K, Lee G, et al. Comparative expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1 in gastric and colorectal cancer. BMC Cancer. 2016;16:358 pubmed publisher
  6. Cabrera J, Lucas J. MAP2 Splicing is Altered in Huntington's Disease. Brain Pathol. 2017;27:181-189 pubmed publisher
  7. Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
  8. Pinto A, Malacrida B, Oieni J, Serafini M, Davin A, Galbiati V, et al. DHEA modulates the effect of cortisol on RACK1 expression via interference with the splicing of the glucocorticoid receptor. Br J Pharmacol. 2015;172:2918-27 pubmed publisher
  9. Eifler T, Shao W, Bartholomeeusen K, Fujinaga K, Jäger S, Johnson J, et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol. 2015;35:468-78 pubmed publisher
  10. Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res. 2013;41:7060-72 pubmed publisher
  11. Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
  12. Walsh C, Suchanek A, Cyphert T, Kohan A, Szeszel Fedorowicz W, Salati L. Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver. J Biol Chem. 2013;288:2816-28 pubmed publisher
  13. Toiber D, Azkona G, Ben Ari S, Toran N, Soreq H, Dierssen M. Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations. Neurobiol Dis. 2010;40:348-59 pubmed publisher
  14. Chandradas S, Deikus G, Tardos J, Bogdanov V. Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 2010;87:147-52 pubmed publisher
  15. Chen Y, Yuo C, Yang W, Jong Y, Lin H, Chang Y, et al. Extracellular pH change modulates the exon 7 splicing in SMN2 mRNA. Mol Cell Neurosci. 2008;39:268-72 pubmed publisher
  16. Yomoda J, Muraki M, Kataoka N, Hosoya T, Suzuki M, Hagiwara M, et al. Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. Genes Cells. 2008;13:233-44 pubmed publisher
  17. Exline C, Feng Z, Stoltzfus C. Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. J Virol. 2008;82:3921-31 pubmed publisher
  18. Liang W, Yuo C, Chang J, Chen Y, Chang Y, Wang H, et al. The effect of hydroxyurea in spinal muscular atrophy cells and patients. J Neurol Sci. 2008;268:87-94 pubmed publisher
  19. Yuo C, Lin H, Chang Y, Yang W, Chang J. 5-(N-ethyl-N-isopropyl)-amiloride enhances SMN2 exon 7 inclusion and protein expression in spinal muscular atrophy cells. Ann Neurol. 2008;63:26-34 pubmed
  20. Huang Y, Kim Y, Li Z, Fomenkov T, Fomenkov A, Ratovitski E. AEC-associated p63 mutations lead to alternative splicing/protein stabilization of p63 and modulation of Notch signaling. Cell Cycle. 2005;4:1440-7 pubmed
  21. Chang J, Hsieh Li H, Jong Y, Wang N, Tsai C, Li H. Treatment of spinal muscular atrophy by sodium butyrate. Proc Natl Acad Sci U S A. 2001;98:9808-13 pubmed