This is a Validated Antibody Database (VAD) review about human SPRED1, based on 7 published articles (read how Labome selects the articles), using SPRED1 antibody in all methods. It is aimed to help Labome visitors find the most suited SPRED1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SPRED1 synonym: NFLS; PPP1R147; hSpred1; spred-1; sprouty-related, EVH1 domain-containing protein 1; protein phosphatase 1, regulatory subunit 147; suppressor of Ras/MAPK activation

Santa Cruz Biotechnology
mouse monoclonal (E-5)
  • immunohistochemistry; human; 1:20; loading ...; fig s3
Santa Cruz Biotechnology SPRED1 antibody (Santa, E-5) was used in immunohistochemistry on human samples at 1:20 (fig s3). Science (2018) ncbi
mouse monoclonal (E-5)
  • western blot; human; tbl 1
Santa Cruz Biotechnology SPRED1 antibody (Santa Cruz, sc-393198) was used in western blot on human samples (tbl 1). Sci Rep (2016) ncbi
Invitrogen
rabbit polyclonal
  • proximity ligation assay; hamsters; loading ...; fig 3d
In order to clarify the interaction of SPRED1 with Ras and Raf, Invitrogen SPRED1 antibody (Thermo Fisher, PA5-20617) was used in proximity ligation assay on hamsters samples (fig 3d). Mol Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 7
  • western blot; mouse; fig 10
In order to investigate the contribution of miR-31 to lung tumorigenesis, Invitrogen SPRED1 antibody (Thermo Fisher, PA5-20617) was used in western blot on human samples (fig 7) and in western blot on mouse samples (fig 10). J Clin Invest (2016) ncbi
Abnova
rabbit polyclonal
  • immunohistochemistry - paraffin section; Rhesus monkey; 1:800
Abnova SPRED1 antibody (Abnova, PAB16582) was used in immunohistochemistry - paraffin section on Rhesus monkey samples at 1:800. J Immunol (2014) ncbi
Abcam
rabbit polyclonal
  • western blot; human; fig 5
  • western blot; mouse; fig 3
Abcam SPRED1 antibody (Abcam Sapphire Bioscience, ab77079) was used in western blot on human samples (fig 5) and in western blot on mouse samples (fig 3). Int J Mol Sci (2016) ncbi
EMD Millipore
rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; fig  S2
EMD Millipore SPRED1 antibody (Millipore, ABS186) was used in immunohistochemistry on mouse samples at 1:500 (fig  S2). J Cell Mol Med (2015) ncbi
Articles Reviewed
  1. Ablain J, Xu M, Rothschild H, JORDAN R, Mito J, Daniels B, et al. Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. Science. 2018;362:1055-1060 pubmed publisher
  2. Siljamäki E, Abankwa D. SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling. Mol Cell Biol. 2016;36:2612-25 pubmed publisher
  3. Wang L, Lee A, Wigg J, Peshavariya H, Liu P, Zhang H. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int J Mol Sci. 2016;17: pubmed publisher
  4. Lombardo G, Dentelli P, Togliatto G, Rosso A, Gili M, Gallo S, et al. Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action. Sci Rep. 2016;6:25689 pubmed publisher
  5. Edmonds M, Boyd K, Moyo T, Mitra R, Duszynski R, Arrate M, et al. MicroRNA-31 initiates lung tumorigenesis and promotes mutant KRAS-driven lung cancer. J Clin Invest. 2016;126:349-64 pubmed publisher
  6. Lei Z, van Mil A, Brandt M, Grundmann S, Hoefer I, Smits M, et al. MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway. J Cell Mol Med. 2015;19:1994-2005 pubmed publisher
  7. Smith A, Wietgrefe S, Shang L, Reilly C, Southern P, Perkey K, et al. Live simian immunodeficiency virus vaccine correlate of protection: immune complex-inhibitory Fc receptor interactions that reduce target cell availability. J Immunol. 2014;193:3126-33 pubmed publisher