This is a Validated Antibody Database (VAD) review about human SPINT1, based on 6 published articles (read how Labome selects the articles), using SPINT1 antibody in all methods. It is aimed to help Labome visitors find the most suited SPINT1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SPINT1 synonym: HAI; HAI1; MANSC2; kunitz-type protease inhibitor 1; HAI-1; hepatocyte growth factor activator inhibitor type 1; serine protease inhibitor, Kunitz type 1

Invitrogen
mouse monoclonal (9B10)
  • immunohistochemistry; human; 1:100; fig 4d
In order to compare the metabolic rates of cytotrophoblast and syncytiotrophoblast cells, Invitrogen SPINT1 antibody (eBiosciences, 9B10) was used in immunohistochemistry on human samples at 1:100 (fig 4d). Sci Rep (2017) ncbi
mouse monoclonal (9B10)
  • immunohistochemistry; human; 1:100; fig 2
In order to discover the unique lipid dynamics in cytotrophoblast cells due to real-time tracking of BODIPY-C12 long-chain fatty acid in human term placenta, Invitrogen SPINT1 antibody (eBioscience, 9B10) was used in immunohistochemistry on human samples at 1:100 (fig 2). PLoS ONE (2016) ncbi
Abcam
rabbit monoclonal (EPR15652)
  • western blot; human; 1:2000; fig 1
Abcam SPINT1 antibody (abcam, ab189511) was used in western blot on human samples at 1:2000 (fig 1). Mol Med Rep (2016) ncbi
R&D Systems
goat polyclonal
  • immunohistochemistry; mouse
  • western blot; mouse
In order to determine the effect of ablating HAI-1 and HAI-2 on endogenous matriptase expression, subcellular localization, and activation in polarized intestinal epithelial cells, R&D Systems SPINT1 antibody (R&D Systems, AF1141) was used in immunohistochemistry on mouse samples and in western blot on mouse samples . J Biol Chem (2014) ncbi
goat polyclonal
  • western blot; human
In order to determine the effect of ablating HAI-1 and HAI-2 on endogenous matriptase expression, subcellular localization, and activation in polarized intestinal epithelial cells, R&D Systems SPINT1 antibody (R&D Systems, AF1048) was used in western blot on human samples . J Biol Chem (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-1)
  • western blot; human
Santa Cruz Biotechnology SPINT1 antibody (Santa Cruz Biotechnology, sc-137159) was used in western blot on human samples . J Biol Chem (2015) ncbi
LifeSpan Biosciences
mouse monoclonal (4H2)
  • immunohistochemistry - paraffin section; human; 1:75
LifeSpan Biosciences SPINT1 antibody (LifeSpan BioSciences, clone 4H2) was used in immunohistochemistry - paraffin section on human samples at 1:75. Cell Death Differ (2015) ncbi
Articles Reviewed
  1. Kolahi K, Valent A, Thornburg K. Cytotrophoblast, Not Syncytiotrophoblast, Dominates Glycolysis and Oxidative Phosphorylation in Human Term Placenta. Sci Rep. 2017;7:42941 pubmed publisher
  2. Sun P, Jiang Z, Chen X, Xue L, Mao X, Ruan G, et al. Decreasing the ratio of matriptase/HAI?1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep. 2016;14:1465-74 pubmed publisher
  3. Kolahi K, Louey S, Varlamov O, Thornburg K. Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells. PLoS ONE. 2016;11:e0153522 pubmed publisher
  4. Sechler M, Borowicz S, Van Scoyk M, Avasarala S, Zerayesus S, Edwards M, et al. Novel Role for γ-Catenin in the Regulation of Cancer Cell Migration via the Induction of Hepatocyte Growth Factor Activator Inhibitor Type 1 (HAI-1). J Biol Chem. 2015;290:15610-20 pubmed publisher
  5. Aghababaei M, Hogg K, Perdu S, Robinson W, Beristain A. ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion. Cell Death Differ. 2015;22:1970-84 pubmed publisher
  6. Friis S, Sales K, Schafer J, Vogel L, Kataoka H, Bugge T. The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin. J Biol Chem. 2014;289:22319-32 pubmed publisher