This is a Validated Antibody Database (VAD) review about human SMURF2, based on 8 published articles (read how Labome selects the articles), using SMURF2 antibody in all methods. It is aimed to help Labome visitors find the most suited SMURF2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D8B8)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 6c
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s7a
Cell Signaling Technology SMURF2 antibody (Cell Signaling, 12024) was used in western blot knockout validation on mouse samples at 1:1000 (fig 6c) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig s7a). Bone Res (2020) ncbi
Abcam
domestic rabbit monoclonal (EP629Y3)
  • western blot; mouse; loading ...; fig 4a
  • western blot; human; loading ...; fig 4a
Abcam SMURF2 antibody (Abcam, EP629Y3) was used in western blot on mouse samples (fig 4a) and in western blot on human samples (fig 4a). PLoS Biol (2018) ncbi
domestic rabbit monoclonal (EP629Y3)
  • western blot; human; 1:1000; fig 5f
In order to assess the definition of obligate participation in multiple hallmarks of cancer by a comprehensive functional characterization of cancer-testis antigens, Abcam SMURF2 antibody (Abcam, ab53316) was used in western blot on human samples at 1:1000 (fig 5f). Nat Commun (2015) ncbi
domestic rabbit monoclonal (EP629Y3)
  • western blot; human
Abcam SMURF2 antibody (Abcam, ab53316) was used in western blot on human samples . FEBS J (2014) ncbi
domestic rabbit monoclonal (EP629Y3)
  • western blot; rat
In order to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats, Abcam SMURF2 antibody (Abcam, ab53316) was used in western blot on rat samples . Obesity (Silver Spring) (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; human; 1:500; fig 2b
Santa Cruz Biotechnology SMURF2 antibody (Santa Cruz, sc-393848) was used in western blot on human samples at 1:500 (fig 2b). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8B8)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology SMURF2 antibody (Cell Signaling, 12024) was used in western blot on human samples (fig 5a). Int J Mol Sci (2020) ncbi
domestic rabbit monoclonal (D8B8)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 6c
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s7a
Cell Signaling Technology SMURF2 antibody (Cell Signaling, 12024) was used in western blot knockout validation on mouse samples at 1:1000 (fig 6c) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig s7a). Bone Res (2020) ncbi
domestic rabbit monoclonal (D8B8)
  • western blot; human; 1:1000; loading ...; fig 4d
Cell Signaling Technology SMURF2 antibody (Cell Signaling Technology, 12024) was used in western blot on human samples at 1:1000 (fig 4d). Cell Death Differ (2019) ncbi
Articles Reviewed
  1. Nam B, Park H, Lee Y, Oh Y, Park J, Kim S, et al. TGFβ1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBPβ-DKK1 Axis. Int J Mol Sci. 2020;21: pubmed publisher
  2. Kushioka J, Kaito T, Okada R, Ishiguro H, Bal Z, Kodama J, et al. A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone. Bone Res. 2020;8:41 pubmed publisher
  3. Gibbs Z, Reza L, Cheng C, Westcott J, McGlynn K, Whitehurst A. The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer. elife. 2020;9: pubmed publisher
  4. Li J, Wang P, Xie Z, Wang S, Cen S, Li M, et al. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2. Cell Death Differ. 2019;: pubmed publisher
  5. Zhu K, Tang Y, Xu X, Dang H, Tang L, Wang X, et al. Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis. PLoS Biol. 2018;16:e3000091 pubmed publisher
  6. Maxfield K, Taus P, Corcoran K, Wooten J, Macion J, Zhou Y, et al. Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer. Nat Commun. 2015;6:8840 pubmed publisher
  7. Choi Y, Kim Y, Jeong H, Jin Y, Yeo C, Lee K. Akt enhances Runx2 protein stability by regulating Smurf2 function during osteoblast differentiation. FEBS J. 2014;281:3656-66 pubmed publisher
  8. Beaudoin M, Snook L, Arkell A, Stefanson A, Wan Z, Simpson J, et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring). 2014;22:1632-42 pubmed publisher