This is a Validated Antibody Database (VAD) review about human SMRT, based on 9 published articles (read how Labome selects the articles), using SMRT antibody in all methods. It is aimed to help Labome visitors find the most suited SMRT antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SMRT synonym: CTG26; N-CoR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1; nuclear receptor corepressor 2; CTG repeat protein 26; T3 receptor-associating factor; silencing mediator for retinoid and thyroid hormone receptors; thyroid-, retinoic-acid-receptor-associated corepressor

Invitrogen
rabbit polyclonal
  • western blot; human; 1:1000; fig 7
In order to study how nuclear corepressor 1 contributes to cancer, Invitrogen SMRT antibody (Affinity Bioreagents, PA1-843) was used in western blot on human samples at 1:1000 (fig 7). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 8a
In order to investigate how PHD2 and PHD3 modulate contractile function, Invitrogen SMRT antibody (Thermo Scientific, PA1-843) was used in immunoprecipitation on human samples (fig 8a). J Clin Invest (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to examine the role of coregulators of nuclear receptor action in thyroid hormone action, Invitrogen SMRT antibody (Thermo Scientific, PA1-843) was used in western blot on mouse samples . Mol Cell Biol (2015) ncbi
rabbit polyclonal
In order to report that SMRT and FOXP1 interact to regulate cardiac growth and macrophage differentiation, Invitrogen SMRT antibody (Affinity Bioreagents, PA1-843) was used . Genes Dev (2008) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1212)
  • chromatin immunoprecipitation; mouse; fig 3c
In order to propose a model in which NCOR1/2 mediates retinoic acid-dependent repression of Fgf8 in caudal progenitors, Santa Cruz Biotechnology SMRT antibody (Santa Cruz, sc-32298) was used in chromatin immunoprecipitation on mouse samples (fig 3c). Dev Biol (2016) ncbi
Abcam
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...; fig 5e
Abcam SMRT antibody (Abcam, ab5802) was used in immunoprecipitation on mouse samples (fig 5e). Cell (2019) ncbi
EMD Millipore
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 7
  • western blot; human; 1:1000; fig 7
In order to study how nuclear corepressor 1 contributes to cancer, EMD Millipore SMRT antibody (Upstate, 06-891) was used in chromatin immunoprecipitation on human samples (fig 7) and in western blot on human samples at 1:1000 (fig 7). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse
In order to examine the role of coregulators of nuclear receptor action in thyroid hormone action, EMD Millipore SMRT antibody (Millipore, 06-891) was used in western blot on mouse samples . Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; African green monkey
EMD Millipore SMRT antibody (Millipore, 06-891) was used in chromatin immunoprecipitation on African green monkey samples . Proc Natl Acad Sci U S A (2013) ncbi
rabbit polyclonal
  • western blot; rat
In order to study the mechanism by which thyroid hormone T3 inhibits the expression of secretory phospholipase A2 group IIa, EMD Millipore SMRT antibody (Millipore, 06-891) was used in western blot on rat samples . J Biol Chem (2013) ncbi
rabbit polyclonal
  • western blot; human; fig 5a
In order to use a novel competition assay to study the spatial and temporal binding of cofactors and reporter genes involved in steroid-regulated transactivation, EMD Millipore SMRT antibody (Millipore, 06-891) was used in western blot on human samples (fig 5a). J Biol Chem (2012) ncbi
Articles Reviewed
  1. Żylicz J, Bousard A, Zumer K, Dossin F, Mohammad E, da Rocha S, et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell. 2019;176:182-197.e23 pubmed publisher
  2. Kumar S, Cunningham T, Duester G. Nuclear receptor corepressors Ncor1 and Ncor2 (Smrt) are required for retinoic acid-dependent repression of Fgf8 during somitogenesis. Dev Biol. 2016;418:204-215 pubmed publisher
  3. Martínez Iglesias O, Alonso Merino E, Gómez Rey S, Velasco Martín J, Martín Orozco R, Luengo E, et al. Autoregulatory loop of nuclear corepressor 1 expression controls invasion, tumor growth, and metastasis. Proc Natl Acad Sci U S A. 2016;113:E328-37 pubmed publisher
  4. Xie L, Pi X, Townley Tilson W, Li N, Wehrens X, Entman M, et al. PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban. J Clin Invest. 2015;125:2759-71 pubmed publisher
  5. Shimizu H, Astapova I, Ye F, Bilban M, Cohen R, Hollenberg A. NCoR1 and SMRT play unique roles in thyroid hormone action in vivo. Mol Cell Biol. 2015;35:555-65 pubmed publisher
  6. Hong S, Dvorak Ewell M, Stevens H, Barish G, Castro G, Nofsinger R, et al. Rescue of a primary myelofibrosis model by retinoid-antagonist therapy. Proc Natl Acad Sci U S A. 2013;110:18820-5 pubmed publisher
  7. Sharma P, Thakran S, Deng X, Elam M, Park E. Nuclear corepressors mediate the repression of phospholipase A2 group IIa gene transcription by thyroid hormone. J Biol Chem. 2013;288:16321-33 pubmed publisher
  8. Blackford J, Guo C, Zhu R, Dougherty E, Chow C, Simons S. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012;287:40982-95 pubmed publisher
  9. Jepsen K, Gleiberman A, Shi C, Simon D, Rosenfeld M. Cooperative regulation in development by SMRT and FOXP1. Genes Dev. 2008;22:740-5 pubmed publisher