This is a Validated Antibody Database (VAD) review about human SMN, based on 10 published articles (read how Labome selects the articles), using SMN antibody in all methods. It is aimed to help Labome visitors find the most suited SMN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SMN synonym: BCD541; GEMIN1; SMA; SMA1; SMA2; SMA3; SMA4; SMA@; SMN; SMNT; T-BCD541; TDRD16A

Abcam
mouse monoclonal (2B1)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 4f
Abcam SMN antibody (Abcam, ab5831) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4f). Theranostics (2021) ncbi
mouse monoclonal (2B1)
  • immunocytochemistry; human; fig 4
Abcam SMN antibody (Abcam, ab5831) was used in immunocytochemistry on human samples (fig 4). Nucleic Acids Res (2015) ncbi
mouse monoclonal (2B1)
  • western blot; mouse
Abcam SMN antibody (Abcam, ab5831) was used in western blot on mouse samples . Stem Cells (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2B1)
  • western blot; human; 1:1000; loading ...; fig 5d
Santa Cruz Biotechnology SMN antibody (Santa Cruz, SC-32313) was used in western blot on human samples at 1:1000 (fig 5d). Nat Commun (2021) ncbi
mouse monoclonal (2B1)
  • western blot; human; 1:200
In order to study the localization of AID and it's associated proteins, Santa Cruz Biotechnology SMN antibody (Santa Cruz, sc-32313) was used in western blot on human samples at 1:200. Exp Cell Res (2014) ncbi
Novus Biologicals
mouse monoclonal (2B1)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 3a
Novus Biologicals SMN antibody (Novus Biologicals, NB100-1936) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 3a). Dis Model Mech (2017) ncbi
mouse monoclonal (2B1)
  • immunocytochemistry; zebrafish ; 1:100; fig s1
  • western blot; zebrafish ; 1:250; fig s1
Novus Biologicals SMN antibody (Novus Biological, NB-1001936) was used in immunocytochemistry on zebrafish samples at 1:100 (fig s1) and in western blot on zebrafish samples at 1:250 (fig s1). Sci Rep (2016) ncbi
Invitrogen
mouse monoclonal (5H1)
  • flow cytometry; mouse; loading ...
In order to describe a method for the retroviral insertion of Bcr-Abl into mice that are genetically depleted for a potential tumor suppressor, Invitrogen SMN antibody (life technologies, 5H1) was used in flow cytometry on mouse samples . Methods Mol Biol (2016) ncbi
MBL International
polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 10 ug/ml
  • western blot; human; 0.5 ug/ml
In order to develop reagents to study RNA binding proteins, MBL International SMN antibody (MBLI, RN077PW) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 10 ug/ml and in western blot on human samples at 0.5 ug/ml. Mol Cell (2016) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (MANSMA1(11F3))
  • immunocytochemistry; human; 1:400; loading ...; fig 1C
  • western blot; human; loading ...; fig 1B
Developmental Studies Hybridoma Bank SMN antibody (DSHB, Mansma1) was used in immunocytochemistry on human samples at 1:400 (fig 1C) and in western blot on human samples (fig 1B). Nucleic Acids Res (2015) ncbi
Articles Reviewed
  1. Hanna R, Flamier A, Barabino A, Bernier G. G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease. Nat Commun. 2021;12:1828 pubmed publisher
  2. Tong W, Hui H, Shang W, Zhang Y, Tian F, Ma Q, et al. Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles. Theranostics. 2021;11:506-521 pubmed publisher
  3. Kobayashi M, Chandrasekhar A, Cheng C, Martinez J, Ng H, de La Hoz C, et al. Diabetic polyneuropathy, sensory neurons, nuclear structure and spliceosome alterations: a role for CWC22. Dis Model Mech. 2017;10:215-224 pubmed publisher
  4. Herrmann O, Schemionek M. Tumor Suppressor Analysis in CML. Methods Mol Biol. 2016;1465:87-94 pubmed publisher
  5. Spiró Z, Koh A, Tay S, See K, Winkler C. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep. 2016;6:27470 pubmed publisher
  6. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  7. Yu Y, Chi B, Xia W, Gangopadhyay J, Yamazaki T, Winkelbauer Hurt M, et al. U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish. Nucleic Acids Res. 2015;43:3208-18 pubmed publisher
  8. Schertzer M, Jouravleva K, Perderiset M, Dingli F, Loew D, Le Guen T, et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Res. 2015;43:1834-47 pubmed publisher
  9. Chen T, Margariti A, Kelaini S, Cochrane A, Guha S, Hu Y, et al. MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling. Stem Cells. 2015;33:1405-18 pubmed publisher
  10. Hu Y, Ericsson I, Doseth B, Liabakk N, Krokan H, Kavli B. Activation-induced cytidine deaminase (AID) is localized to subnuclear domains enriched in splicing factors. Exp Cell Res. 2014;322:178-92 pubmed publisher