This is a Validated Antibody Database (VAD) review about human SLC8A1, based on 21 published articles (read how Labome selects the articles), using SLC8A1 antibody in all methods. It is aimed to help Labome visitors find the most suited SLC8A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SLC8A1 synonym: NCX1; sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; solute carrier family 8 (sodium/calcium exchanger), member 1; solute carrier family 8 member 1

Knockout validation
SWant
mouse monoclonal
  • western blot knockout validation; mouse; fig 1
SWant SLC8A1 antibody (ind, R3F1) was used in western blot knockout validation on mouse samples (fig 1). Physiol Rep (2015) ncbi
Invitrogen
mouse monoclonal (C2C12)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Invitrogen SLC8A1 antibody (ThermoFisher, MA3-926) was used in western blot on mouse samples at 1:1000 (fig 4b). elife (2019) ncbi
mouse monoclonal (C2C12)
  • western blot; mouse; loading ...; fig 7c
In order to test if in utero particulate matter exposure alters cardiac structure and function at adulthood, Invitrogen SLC8A1 antibody (Thermo Fisher, MA3-926) was used in western blot on mouse samples (fig 7c). J Am Heart Assoc (2017) ncbi
mouse monoclonal (C2C12)
  • western blot; human; 1:1000; loading ...; fig 1a
In order to discuss how mutations in sarcomere genes may result in altered calcium signaling and heart defects, Invitrogen SLC8A1 antibody (Thermo Pierce, MA3-926) was used in western blot on human samples at 1:1000 (fig 1a). Circulation (2016) ncbi
mouse monoclonal (C2C12)
  • immunocytochemistry; pig; 1:200; fig 6
In order to characterize modulation of ryanodine receptors during beta-adrenergic stimulation and restriction to the dyadic cleft via nictric oxide synthase-1 and calcium/calmodulin-dependent kinase II, Invitrogen SLC8A1 antibody (Thermo Scientific, MA3-926) was used in immunocytochemistry on pig samples at 1:200 (fig 6). J Physiol (2016) ncbi
mouse monoclonal (C2C12)
  • western blot; mouse; loading ...; fig 7g
In order to study the role of angiotensin receptor-associated protein Atrap on cardiac Ca2+-ATPase SERCA2a, Invitrogen SLC8A1 antibody (ThermoFisher, MA3-926) was used in western blot on mouse samples (fig 7g). Cardiovasc Res (2016) ncbi
mouse monoclonal (C2C12)
  • immunocytochemistry; dog; 1:100; fig s14
In order to study dyssynchronous heart failure after cardiac resynchronization therapy, Invitrogen SLC8A1 antibody (ThermoFisher Scientific, MA3-926) was used in immunocytochemistry on dog samples at 1:100 (fig s14). Circ Heart Fail (2015) ncbi
mouse monoclonal (C2C12)
  • immunocytochemistry; mouse; 1:100
In order to study if transient receptor potential vanilloid channels type 2 form subunits of the dysregulated stretch-activated channels in cardiac dystrophy, Invitrogen SLC8A1 antibody (Thermo Scientific, MA3-926) was used in immunocytochemistry on mouse samples at 1:100. Cardiovasc Res (2015) ncbi
mouse monoclonal (C2C12)
  • western blot; rabbit
In order to study the effect of progesterone on SERCA2a regulation in isolated cardiomyocytes, Invitrogen SLC8A1 antibody (Thermo Scientific, MA3-926) was used in western blot on rabbit samples . Am J Physiol Cell Physiol (2014) ncbi
Abcam
mouse monoclonal (C2C12)
  • western blot; pig
Abcam SLC8A1 antibody (Abcam, ab2869) was used in western blot on pig samples . PLoS ONE (2015) ncbi
mouse monoclonal (C2C12)
  • flow cytometry; human; fig 1
  • immunocytochemistry; human
Abcam SLC8A1 antibody (Abcam, ab2869) was used in flow cytometry on human samples (fig 1) and in immunocytochemistry on human samples . PLoS ONE (2013) ncbi
Alomone Labs
rabbit polyclonal
  • western blot; mouse; fig 4
In order to determine the impact of ALDH2 overexpression on doxorubicin-induced myocardial damage and mitochondria, Alomone Labs SLC8A1 antibody (Alomone labs, ANX-011) was used in western blot on mouse samples (fig 4). Biochim Biophys Acta (2016) ncbi
SWant
mouse monoclonal
  • immunohistochemistry - frozen section; sheep; 1:200; loading ...; fig 4
In order to describe transverse-tubule development in the large mammalian fetal heart, SWant SLC8A1 antibody (Swant, R3F1) was used in immunohistochemistry - frozen section on sheep samples at 1:200 (fig 4). J Muscle Res Cell Motil (2016) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...
In order to explore the role of junctophilin-2 in the ventricular muscle cells of the heart, SWant SLC8A1 antibody (Swant, R3F1) was used in western blot on mouse samples at 1:1000. J Cell Sci (2016) ncbi
mouse monoclonal
  • western blot; rat; fig 2
In order to study the effects of ascending aortic banding on calcium handling in rat isolated atrial myocytes, SWant SLC8A1 antibody (Swannt, R3F1) was used in western blot on rat samples (fig 2). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
SWant SLC8A1 antibody (Swant, p 11?C13) was used in western blot on human samples at 1:1000 (fig 3). J Neurosci (2015) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; 1:200; tbl 1
In order to examine the interaction between store-operated calcium entry and store content and its role in cardiac pacemaking, SWant SLC8A1 antibody (Swant, R3F1) was used in immunocytochemistry on mouse samples at 1:200 (tbl 1). Front Physiol (2015) ncbi
mouse monoclonal
  • western blot knockout validation; mouse; fig 1
SWant SLC8A1 antibody (ind, R3F1) was used in western blot knockout validation on mouse samples (fig 1). Physiol Rep (2015) ncbi
mouse monoclonal
  • immunohistochemistry; rat; 1:100
In order to quantify the spatial distribution ryanodine receptors throughout the myoplasm, SWant SLC8A1 antibody (SWANT, R3F1) was used in immunohistochemistry on rat samples at 1:100. J Mol Cell Cardiol (2015) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000
In order to study the loss of thd miR-106b-25 cluster in the pathogenesis of atrial fibrillation, SWant SLC8A1 antibody (Swant, R3F1) was used in western blot on mouse samples at 1:1000. Circ Arrhythm Electrophysiol (2014) ncbi
mouse monoclonal
  • western blot; mouse; fig 6
In order to suggest that the inability to enhance myofilament relaxation through cTnI phosphorylation predisposes the heart to hypertrophic cardiomyopathy, SWant SLC8A1 antibody (SWANT, R3F1) was used in western blot on mouse samples (fig 6). J Biol Chem (2014) ncbi
mouse monoclonal
  • immunocytochemistry; rat; 1:500
SWant SLC8A1 antibody (Swant, R3F1) was used in immunocytochemistry on rat samples at 1:500. Am J Physiol Cell Physiol (2013) ncbi
Articles Reviewed
  1. Dai W, Laforest B, Tyan L, Shen K, Nadadur R, Alvarado F, et al. A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. elife. 2019;8: pubmed publisher
  2. Tanwar V, Gorr M, Velten M, Eichenseer C, Long V, Bonilla I, et al. In Utero Particulate Matter Exposure Produces Heart Failure, Electrical Remodeling, and Epigenetic Changes at Adulthood. J Am Heart Assoc. 2017;6: pubmed publisher
  3. Munro M, Soeller C. Early transverse tubule development begins in utero in the sheep heart. J Muscle Res Cell Motil. 2016;37:195-202 pubmed publisher
  4. Munro M, Jayasinghe I, Wang Q, Quick A, Wang W, Baddeley D, et al. Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes. J Cell Sci. 2016;129:4388-4398 pubmed
  5. Helms A, Alvarado F, Yob J, Tang V, Pagani F, Russell M, et al. Genotype-Dependent and -Independent Calcium Signaling Dysregulation in Human Hypertrophic Cardiomyopathy. Circulation. 2016;134:1738-1748 pubmed
  6. Dries E, Santiago D, Johnson D, Gilbert G, Holemans P, Korte S, et al. Calcium/calmodulin-dependent kinase II and nitric oxide synthase 1-dependent modulation of ryanodine receptors during ?-adrenergic stimulation is restricted to the dyadic cleft. J Physiol. 2016;594:5923-5939 pubmed publisher
  7. Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach K, Grill A, et al. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a. Cardiovasc Res. 2016;110:359-70 pubmed publisher
  8. Zhang H, Cannell M, Kim S, Watson J, Norman R, Calaghan S, et al. Cellular Hypertrophy and Increased Susceptibility to Spontaneous Calcium-Release of Rat Left Atrial Myocytes Due to Elevated Afterload. PLoS ONE. 2015;10:e0144309 pubmed publisher
  9. Ge W, Yuan M, Ceylan A, Wang X, Ren J. Mitochondrial aldehyde dehydrogenase protects against doxorubicin cardiotoxicity through a transient receptor potential channel vanilloid 1-mediated mechanism. Biochim Biophys Acta. 2016;1862:622-634 pubmed publisher
  10. Li H, Lichter J, Seidel T, Tomaselli G, Bridge J, Sachse F. Cardiac Resynchronization Therapy Reduces Subcellular Heterogeneity of Ryanodine Receptors, T-Tubules, and Ca2+ Sparks Produced by Dyssynchronous Heart Failure. Circ Heart Fail. 2015;8:1105-14 pubmed publisher
  11. Formisano L, Guida N, Valsecchi V, Cantile M, Cuomo O, Vinciguerra A, et al. Sp3/REST/HDAC1/HDAC2 Complex Represses and Sp1/HIF-1/p300 Complex Activates ncx1 Gene Transcription, in Brain Ischemia and in Ischemic Brain Preconditioning, by Epigenetic Mechanism. J Neurosci. 2015;35:7332-48 pubmed publisher
  12. Liu J, Xin L, Benson V, Allen D, Ju Y. Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells. Front Physiol. 2015;6:69 pubmed publisher
  13. Lugenbiel P, Wenz F, Govorov K, Schweizer P, Katus H, Thomas D. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS ONE. 2015;10:e0116395 pubmed publisher
  14. Wang Y, Chen L, Li M, Cha H, Iwamoto T, Zhang J. Conditional knockout of smooth muscle sodium calcium exchanger type-1 lowers blood pressure and attenuates Angiotensin II-salt hypertension. Physiol Rep. 2015;3: pubmed publisher
  15. Lorin C, Vögeli I, Niggli E. Dystrophic cardiomyopathy: role of TRPV2 channels in stretch-induced cell damage. Cardiovasc Res. 2015;106:153-62 pubmed publisher
  16. Hou Y, Jayasinghe I, Crossman D, Baddeley D, Soeller C. Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes. J Mol Cell Cardiol. 2015;80:45-55 pubmed publisher
  17. Chiang D, Kongchan N, Beavers D, Alsina K, Voigt N, Neilson J, et al. Loss of microRNA-106b-25 cluster promotes atrial fibrillation by enhancing ryanodine receptor type-2 expression and calcium release. Circ Arrhythm Electrophysiol. 2014;7:1214-22 pubmed publisher
  18. Moshal K, Zhang Z, Roder K, Kim T, Cooper L, Patedakis Litvinov B, et al. Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes. Am J Physiol Cell Physiol. 2014;307:C1050-7 pubmed publisher
  19. Dweck D, Sanchez Gonzalez M, Chang A, Dulce R, Badger C, Koutnik A, et al. Long term ablation of protein kinase A (PKA)-mediated cardiac troponin I phosphorylation leads to excitation-contraction uncoupling and diastolic dysfunction in a knock-in mouse model of hypertrophic cardiomyopathy. J Biol Chem. 2014;289:23097-111 pubmed publisher
  20. Ho W, Davis A, Chadha P, Greenwood I. Effective contractile response to voltage-gated Na+ channels revealed by a channel activator. Am J Physiol Cell Physiol. 2013;304:C739-47 pubmed publisher
  21. Raynaud C, Halabi N, Elliott D, Pasquier J, Elefanty A, Stanley E, et al. Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes. PLoS ONE. 2013;8:e54524 pubmed publisher