This is a Validated Antibody Database (VAD) review about human SLC1A3, based on 28 published articles (read how Labome selects the articles), using SLC1A3 antibody in all methods. It is aimed to help Labome visitors find the most suited SLC1A3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SLC1A3 synonym: EA6; EAAT1; GLAST; GLAST1; excitatory amino acid transporter 1; GLAST-1; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3

Abcam
rabbit monoclonal (EPR12686)
  • western blot; rat; 1:1000; loading ...; fig 3b
Abcam SLC1A3 antibody (Abcam, EPR12686) was used in western blot on rat samples at 1:1000 (fig 3b). Neurotoxicology (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 7b
In order to find amino acids important for sorting nexin 27 interactions, Abcam SLC1A3 antibody (Abcam, Ab416) was used in western blot on human samples at 1:1000 (fig 7b). Nat Struct Mol Biol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 5a
  • western blot; mouse; 1:1500; fig 9
In order to examine synaptic connectivity and dysmyelination during cerebellar morphogenesis in Npc1 (nmf164) homozygous mice, Abcam SLC1A3 antibody (AbCam, ab416) was used in immunohistochemistry on mouse samples at 1:250 (fig 5a) and in western blot on mouse samples at 1:1500 (fig 9). Acta Neuropathol Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; fig 2
Abcam SLC1A3 antibody (Abcam, AB416) was used in immunocytochemistry on mouse samples at 1:200 (fig 2). Biomed Res Int (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 3c
Abcam SLC1A3 antibody (Abcam, ab416) was used in western blot on mouse samples at 1:500 (fig 3c). Toxicol Appl Pharmacol (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...
In order to investigate the contribution of YAP in mouse neocortical astrocytic differentiation and proliferation, Abcam SLC1A3 antibody (Abcam, AB416) was used in immunocytochemistry on mouse samples at 1:200. Development (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig s2a
In order to examine Sonic hedgehog signaling in the human fetal neocortex, Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry - frozen section on mouse samples at 1:400 (fig s2a). Nat Neurosci (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:100; fig 8e
  • immunohistochemistry; rat; 1:100; fig 8b
In order to study how transcriptional regulation and Skp2-mediated degradation of p27Kip1 activates stem cell properties of muller glia through notch signaling, Abcam SLC1A3 antibody (Abcam, ab416) was used in immunocytochemistry on rat samples at 1:100 (fig 8e) and in immunohistochemistry on rat samples at 1:100 (fig 8b). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig s1
Abcam SLC1A3 antibody (Abcam, ab-416) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s1). Science (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:500
Abcam SLC1A3 antibody (Abcam, ab416) was used in immunocytochemistry on mouse samples at 1:500. J Neurosci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:200
Abcam SLC1A3 antibody (AbCam, ab416) was used in immunocytochemistry on rat samples at 1:200. Exp Eye Res (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 8
In order to investigate the role of Sox-9 in the maintenance and fate decision of the neural progenitor pool in the cerebellum, Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry on mouse samples at 1:250 (fig 8). Mol Brain (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:400; loading ...; fig 4b
Abcam SLC1A3 antibody (Abcam, 416) was used in immunohistochemistry on human samples at 1:400 (fig 4b). Front Neurosci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
Abcam SLC1A3 antibody (Abcam, ab416) was used in immunocytochemistry on mouse samples . Stem Cells Dev (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:2000
  • western blot; mouse; fig 6
  • immunohistochemistry - frozen section; human; 1:2000
  • western blot; human
Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry - frozen section on mouse samples at 1:2000, in western blot on mouse samples (fig 6), in immunohistochemistry - frozen section on human samples at 1:2000 and in western blot on human samples . Neuroscience (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100
Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry - frozen section on mouse samples at 1:100. PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100
In order to study the conversion inner border cells and inner phalangeal cells to inner hair cells, Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry on mouse samples at 1:100. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:4,000
Abcam SLC1A3 antibody (Abcam, ab416) was used in western blot on rat samples at 1:4,000. Biomaterials (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:500
Abcam SLC1A3 antibody (Abcam, ab416) was used in immunocytochemistry on mouse samples at 1:500. Nat Med (2013) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Abcam SLC1A3 antibody (Abcam, ab416) was used in western blot on mouse samples at 1:1000. PLoS ONE (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:1000
In order to study the expression of CB1 cannabinoid receptor in the ependyma of the rat spinal cord, Abcam SLC1A3 antibody (Abcam, ab416) was used in immunohistochemistry on rat samples at 1:1000. J Comp Neurol (2013) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig s1a
In order to study brain apoE regulation, Invitrogen SLC1A3 antibody (Thermo Scientific, PA5-19709) was used in immunocytochemistry on human samples (fig s1a). Cell Chem Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3c
In order to examine the effects of sleep and wake on astrocytes, Invitrogen SLC1A3 antibody (Thermo Scientific, PA5-19709) was used in western blot on mouse samples at 1:1000 (fig 3c). BMC Biol (2015) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • western blot; Domestic guinea pig; 1:1000; fig 3b
In order to study the effects of sarin on the guinea pig brainstem, LifeSpan Biosciences SLC1A3 antibody (LifeSpan BioSciences, LS-A9447) was used in western blot on Domestic guinea pig samples at 1:1000 (fig 3b). Brain Res (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D44E2)
  • western blot; mouse; loading ...; fig 2a
In order to find that the Glu/Asp transporter SLC1A3 is localized to the plasma membrane and the major regulator of acidic amino acid uptake in adipocytes, Cell Signaling Technology SLC1A3 antibody (Cell Signaling, 5684) was used in western blot on mouse samples (fig 2a). FEBS Lett (2017) ncbi
rabbit monoclonal (D44E2)
  • western blot; human; loading ...; tbl 4
Cell Signaling Technology SLC1A3 antibody (Cell signaling, 5684) was used in western blot on human samples (tbl 4). Transl Psychiatry (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
Cell Signaling Technology SLC1A3 antibody (Cell Signaling Technology, 4166) was used in western blot on rat samples at 1:1000. Neurochem Res (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Cell Signaling Technology SLC1A3 antibody (Cell Signaling, 4166S) was used in western blot on mouse samples at 1:1000. Neurobiol Learn Mem (2014) ncbi
Articles Reviewed
  1. Krycer J, Fazakerley D, Cater R, C Thomas K, Naghiloo S, Burchfield J, et al. The amino acid transporter, SLC1A3, is plasma membrane-localised in adipocytes and its activity is insensitive to insulin. FEBS Lett. 2017;591:322-330 pubmed publisher
  2. Lopes M, Leal R, Guarnieri R, Schwarzbold M, Hoeller A, Diaz A, et al. A single high dose of dexamethasone affects the phosphorylation state of glutamate AMPA receptors in the human limbic system. Transl Psychiatry. 2016;6:e986 pubmed publisher
  3. FINAN G, Realubit R, Chung S, Lutjohann D, Wang N, Cirrito J, et al. Bioactive Compound Screen for Pharmacological Enhancers of Apolipoprotein E in Primary Human Astrocytes. Cell Chem Biol. 2016;23:1526-1538 pubmed publisher
  4. Mohammadi M, Zare Z, Allah Moradi E, Vaezi N, Valadan R, Tehrani M. Alterations in mRNA and protein expression of glutamate transporters in rat hippocampus after paraoxon exposure. Neurotoxicology. 2016;57:251-257 pubmed publisher
  5. Clairfeuille T, Mas C, Chan A, Yang Z, Tello Lafoz M, Chandra M, et al. A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex. Nat Struct Mol Biol. 2016;23:921-932 pubmed publisher
  6. Caporali P, Bruno F, Palladino G, Dragotto J, Petrosini L, Mangia F, et al. Developmental delay in motor skill acquisition in Niemann-Pick C1 mice reveals abnormal cerebellar morphogenesis. Acta Neuropathol Commun. 2016;4:94 pubmed publisher
  7. Skytt D, Toft Kehler A, Brændstrup C, Cejvanovic S, Gurubaran I, Bergersen L, et al. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. Biomed Res Int. 2016;2016:1087647 pubmed publisher
  8. Wang Y, Chen M, Zhang Y, Huo T, Fang Y, Jiao X, et al. Effects of realgar on GSH synthesis in the mouse hippocampus: Involvement of system XAG(-), system XC(-), MRP-1 and Nrf2. Toxicol Appl Pharmacol. 2016;308:91-101 pubmed publisher
  9. Huang Z, Hu J, Pan J, Wang Y, Hu G, Zhou J, et al. YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation. Development. 2016;143:2398-409 pubmed publisher
  10. Wang L, Hou S, Han Y. Hedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex. Nat Neurosci. 2016;19:888-96 pubmed publisher
  11. Del Debbio C, Mir Q, Parameswaran S, Mathews S, Xia X, Zheng L, et al. Notch Signaling Activates Stem Cell Properties of Müller Glia through Transcriptional Regulation and Skp2-mediated Degradation of p27Kip1. PLoS ONE. 2016;11:e0152025 pubmed publisher
  12. Farmer W, Abrahamsson T, Chierzi S, Lui C, Zaelzer C, Jones E, et al. Neurons diversify astrocytes in the adult brain through sonic hedgehog signaling. Science. 2016;351:849-54 pubmed publisher
  13. Bellesi M, de Vivo L, Tononi G, Cirelli C. Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies. BMC Biol. 2015;13:66 pubmed publisher
  14. Liu Y, Miao Q, Yuan J, Han S, Zhang P, Li S, et al. Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo. J Neurosci. 2015;35:9336-55 pubmed publisher
  15. Kaja S, Payne A, Naumchuk Y, Levy D, Zaidi D, Altman A, et al. Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes. Exp Eye Res. 2015;138:159-66 pubmed publisher
  16. Vong K, Leung C, Behringer R, Kwan K. Sox9 is critical for suppression of neurogenesis but not initiation of gliogenesis in the cerebellum. Mol Brain. 2015;8:25 pubmed publisher
  17. Brøchner C, Holst C, Møllgård K. Outer brain barriers in rat and human development. Front Neurosci. 2015;9:75 pubmed publisher
  18. Meade M, Hoffmann A, Makley M, Snider T, Schlager J, Gearhart J. Quantitative proteomic analysis of the brainstem following lethal sarin exposure. Brain Res. 2015;1611:101-13 pubmed publisher
  19. Gao C, Zhu W, Tian L, Zhang J, Li Z. MCT4-mediated expression of EAAT1 is involved in the resistance to hypoxia injury in astrocyte-neuron co-cultures. Neurochem Res. 2015;40:818-28 pubmed publisher
  20. Louhivuori L, Jansson L, Turunen P, Jäntti M, Nordström T, Louhivuori V, et al. Transient receptor potential channels and their role in modulating radial glial-neuronal interaction: a signaling pathway involving mGluR5. Stem Cells Dev. 2015;24:701-13 pubmed publisher
  21. Roberts R, Roche J, McCullumsmith R. Localization of excitatory amino acid transporters EAAT1 and EAAT2 in human postmortem cortex: a light and electron microscopic study. Neuroscience. 2014;277:522-40 pubmed publisher
  22. Bogdanov P, Corraliza L, Villena J, Carvalho A, Garcia Arumi J, Ramos D, et al. The db/db mouse: a useful model for the study of diabetic retinal neurodegeneration. PLoS ONE. 2014;9:e97302 pubmed publisher
  23. Wang Z, Park S, Zhao H, Peng S, Zuo Z. A critical role of glutamate transporter type 3 in the learning and memory of mice. Neurobiol Learn Mem. 2014;114:70-80 pubmed publisher
  24. Liu Z, Fang J, Dearman J, Zhang L, Zuo J. In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression. PLoS ONE. 2014;9:e89377 pubmed publisher
  25. Zuidema J, Hyzinski García M, Van Vlasselaer K, Zaccor N, Plopper G, Mongin A, et al. Enhanced GLT-1 mediated glutamate uptake and migration of primary astrocytes directed by fibronectin-coated electrospun poly-L-lactic acid fibers. Biomaterials. 2014;35:1439-49 pubmed publisher
  26. Bittner S, Ruck T, Schuhmann M, Herrmann A, Moha Ou Maati H, Bobak N, et al. Endothelial TWIK-related potassium channel-1 (TREK1) regulates immune-cell trafficking into the CNS. Nat Med. 2013;19:1161-5 pubmed publisher
  27. Povlsen J, Løfgren B, Dalgas C, Birkler R, Johannsen M, Støttrup N, et al. Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation. PLoS ONE. 2013;8:e64093 pubmed publisher
  28. Garcia Ovejero D, Arevalo Martin A, Paniagua Torija B, Sierra Palomares Y, Molina Holgado E. A cell population that strongly expresses the CB1 cannabinoid receptor in the ependyma of the rat spinal cord. J Comp Neurol. 2013;521:233-51 pubmed publisher