This is a Validated Antibody Database (VAD) review about human SIRT5, based on 5 published articles (read how Labome selects the articles), using SIRT5 antibody in all methods. It is aimed to help Labome visitors find the most suited SIRT5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIRT5 synonym: SIR2L5

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D8C3)
  • western blot knockout validation; mouse; fig 3
  • western blot; human; fig 3
Cell Signaling Technology SIRT5 antibody (Cell Signaling, 8782) was used in western blot knockout validation on mouse samples (fig 3) and in western blot on human samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Invitrogen
mouse recombinant
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen SIRT5 antibody (life tech, 730092) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D8C3)
  • western blot; human; loading ...; fig s2
Cell Signaling Technology SIRT5 antibody (CST, 8782) was used in western blot on human samples (fig s2). Cell Commun Signal (2019) ncbi
domestic rabbit monoclonal (D5E11)
  • western blot; human; 1:2000; loading ...; fig 4a, 4b, 4c
Cell Signaling Technology SIRT5 antibody (Cell Signaling, D5E11) was used in western blot on human samples at 1:2000 (fig 4a, 4b, 4c). Front Oncol (2019) ncbi
domestic rabbit monoclonal (D8C3)
  • western blot; mouse; 1:500; loading ...; fig 1e
Cell Signaling Technology SIRT5 antibody (Cell Signaling Technology, 8782) was used in western blot on mouse samples at 1:500 (fig 1e). Sci Rep (2019) ncbi
domestic rabbit monoclonal (D8C3)
  • western blot knockout validation; mouse; fig 3
  • western blot; human; fig 3
Cell Signaling Technology SIRT5 antibody (Cell Signaling, 8782) was used in western blot knockout validation on mouse samples (fig 3) and in western blot on human samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Articles Reviewed
  1. Zhou H, Zeng H, Yuan D, Ren J, Cheng S, Yu H, et al. NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma. Cell Commun Signal. 2019;17:168 pubmed publisher
  2. Garva R, Thepmalee C, Yasamut U, Sudsaward S, Guazzelli A, Rajendran R, et al. Sirtuin Family Members Selectively Regulate Autophagy in Osteosarcoma and Mesothelioma Cells in Response to Cellular Stress. Front Oncol. 2019;9:949 pubmed publisher
  3. Lin J, Lin J, Chen H, Chen T, Apte R. Combined SIRT3 and SIRT5 deletion is associated with inner retinal dysfunction in a mouse model of type 1 diabetes. Sci Rep. 2019;9:3799 pubmed publisher
  4. Sadhukhan S, Liu X, Ryu D, Nelson O, Stupinski J, Li Z, et al. Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function. Proc Natl Acad Sci U S A. 2016;113:4320-5 pubmed publisher
  5. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher