This is a Validated Antibody Database (VAD) review about human SIRT3, based on 30 published articles (read how Labome selects the articles), using SIRT3 antibody in all methods. It is aimed to help Labome visitors find the most suited SIRT3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIRT3 synonym: SIR2L3

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (C73E3)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1m
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627) was used in western blot knockout validation on human samples at 1:1000 (fig 1m). Nat Commun (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; human; loading ...; fig 1a
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in western blot knockout validation on human samples (fig 1a). Cell Rep (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; loading ...; fig 6b
Cell Signaling Technology SIRT3 antibody (Cell signaling, 5490) was used in western blot knockout validation on mouse samples (fig 6b). J Mol Cell Cardiol (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; loading ...; fig 1a
  • immunoprecipitation; mouse; loading ...; fig 5e
  • western blot; rat; fig 3d
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot knockout validation on mouse samples (fig 1a), in immunoprecipitation on mouse samples (fig 5e) and in western blot on rat samples (fig 3d). Oncotarget (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; fig 1
Cell Signaling Technology SIRT3 antibody (Cell signaling, 5490) was used in western blot knockout validation on mouse samples (fig 1). J Cell Mol Med (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-10)
  • western blot; human; 1:100; loading ...; fig 2
Santa Cruz Biotechnology SIRT3 antibody (Santa Cruz, sc-365175) was used in western blot on human samples at 1:100 (fig 2). Oncotarget (2016) ncbi
mouse monoclonal (F-10)
  • immunocytochemistry; human; 1:50; fig 2
  • western blot; human
Santa Cruz Biotechnology SIRT3 antibody (Santa Cruz, sc-365175) was used in immunocytochemistry on human samples at 1:50 (fig 2) and in western blot on human samples . Age (Dordr) (2013) ncbi
Proteintech Group
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2d, 7c
Proteintech Group SIRT3 antibody (Nordic Biosite, 10099-1-AP) was used in western blot on human samples (fig 2d, 7c). Nucleic Acids Res (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C73E3)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1b, 2c
  • western blot; human; 1:1000; loading ...; fig s4
Cell Signaling Technology SIRT3 antibody (CST, C73E3) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1b, 2c) and in western blot on human samples at 1:1000 (fig s4). BMC Cancer (2020) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; human; loading ...; fig s2
Cell Signaling Technology SIRT3 antibody (CST, 2627) was used in western blot on human samples (fig s2). Cell Commun Signal (2019) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; human; 1:2000; loading ...; fig 3a, 3b, 3c
Cell Signaling Technology SIRT3 antibody (Cell Signaling, C73E3) was used in western blot on human samples at 1:2000 (fig 3a, 3b, 3c). Front Oncol (2019) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; 1:1000; loading ...; fig 1e
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490S) was used in western blot on mouse samples at 1:1000 (fig 1e). Sci Rep (2019) ncbi
domestic rabbit monoclonal (D22A3)
  • immunohistochemistry; mouse; loading ...; fig 1b
  • western blot; mouse; fig 2d, 2e
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in immunohistochemistry on mouse samples (fig 1b) and in western blot on mouse samples (fig 2d, 2e). JCI Insight (2019) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1m
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627) was used in western blot knockout validation on human samples at 1:1000 (fig 1m). Nat Commun (2018) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; human; loading ...; fig 1a
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in western blot knockout validation on human samples (fig 1a). Cell Rep (2018) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; mouse; loading ...; fig 6a
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 2627) was used in western blot on mouse samples (fig 6a). Cell Rep (2018) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; human; fig s5f
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in western blot on human samples (fig s5f). Cell (2018) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; 1:1000; loading ...; fig s3s5
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot on mouse samples at 1:1000 (fig s3s5). elife (2018) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot on mouse samples (fig 5a). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; loading ...; fig 6b
Cell Signaling Technology SIRT3 antibody (Cell signaling, 5490) was used in western blot knockout validation on mouse samples (fig 6b). J Mol Cell Cardiol (2017) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; mouse; 1:1000; loading ...; fig 4a
In order to elucidate the mechanism by which the I4895T mutation in the type 1 ryanodine receptor/Ca(2+) release channel results in disease, Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627) was used in western blot on mouse samples at 1:1000 (fig 4a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; 1:1000; fig 5a
In order to study the contribution of Id1 to brown adipose tissue thermogenesis and to the regulation of beige adipocytes in white adipose tissue, Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot on mouse samples at 1:1000 (fig 5a). Diabetes (2017) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; loading ...; fig 6
Cell Signaling Technology SIRT3 antibody (Cell Signaling, D22A3) was used in western blot on mouse samples (fig 6). Am J Physiol Endocrinol Metab (2017) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; loading ...; fig 1a
  • immunoprecipitation; mouse; loading ...; fig 5e
  • western blot; rat; fig 3d
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot knockout validation on mouse samples (fig 1a), in immunoprecipitation on mouse samples (fig 5e) and in western blot on rat samples (fig 3d). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; loading ...; fig 4a
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in western blot on mouse samples (fig 4a). J Exp Med (2016) ncbi
domestic rabbit monoclonal (C73E3)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 9a
  • western blot; human; fig 2a
In order to test if SIRT3 regulates succinate dehydrogenase activity, succinate levels, and GPR91 expression in human stem cells and in a nonalcoholic fatty liver disease model, Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 9a) and in western blot on human samples (fig 2a). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse; fig 4
Cell Signaling Technology SIRT3 antibody (Cell signaling, 5490) was used in western blot on mouse samples (fig 4). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; human; fig 1a
  • western blot; mouse; fig 1a
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627s) was used in western blot on human samples (fig 1a) and in western blot on mouse samples (fig 1a). Mol Cancer Ther (2015) ncbi
domestic rabbit monoclonal (C73E3)
  • immunohistochemistry; human; 1:50; fig 1
Cell Signaling Technology SIRT3 antibody (Cell Signalling Technology, #2627) was used in immunohistochemistry on human samples at 1:50 (fig 1). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; human
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 2627) was used in western blot on human samples . Biomed Res Int (2015) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse
In order to test the requirement of AMP-activated protein kinase for exercise training-induced increases in skeletal muscle abundance of mitochondrial proteins, Cell Signaling Technology SIRT3 antibody (Cell Signaling, #5490S) was used in western blot on mouse samples . Front Physiol (2015) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot knockout validation; mouse; fig 1
Cell Signaling Technology SIRT3 antibody (Cell signaling, 5490) was used in western blot knockout validation on mouse samples (fig 1). J Cell Mol Med (2015) ncbi
domestic rabbit monoclonal (C73E3)
  • western blot; human; fig s3
Cell Signaling Technology SIRT3 antibody (Cell Signaling Tech, 2627) was used in western blot on human samples (fig s3). Autophagy (2015) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; human; 1:1000
Cell Signaling Technology SIRT3 antibody (Cell Signaling Technology, 5490) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; mouse
In order to study the mechanism underlying the preventative effect of uridine against fenofibrate-induced fatty liver disease, Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D22A3)
  • western blot; human
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490) was used in western blot on human samples . Anal Chem (2013) ncbi
domestic rabbit monoclonal (D22A3)
  • immunocytochemistry; human; 1:50
  • western blot; human
Cell Signaling Technology SIRT3 antibody (Cell Signaling, 5490 s) was used in immunocytochemistry on human samples at 1:50 and in western blot on human samples . Age (Dordr) (2013) ncbi
Articles Reviewed
  1. Jo H, Park Y, Kim T, Kim J, Lee J, Kim S, et al. Modulation of SIRT3 expression through CDK4/6 enhances the anti-cancer effect of sorafenib in hepatocellular carcinoma cells. BMC Cancer. 2020;20:332 pubmed publisher
  2. Zhou H, Zeng H, Yuan D, Ren J, Cheng S, Yu H, et al. NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma. Cell Commun Signal. 2019;17:168 pubmed publisher
  3. Garva R, Thepmalee C, Yasamut U, Sudsaward S, Guazzelli A, Rajendran R, et al. Sirtuin Family Members Selectively Regulate Autophagy in Osteosarcoma and Mesothelioma Cells in Response to Cellular Stress. Front Oncol. 2019;9:949 pubmed publisher
  4. Zheng J, Croteau D, Bohr V, Akbari M. Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells. Nucleic Acids Res. 2019;: pubmed publisher
  5. Lin J, Lin J, Chen H, Chen T, Apte R. Combined SIRT3 and SIRT5 deletion is associated with inner retinal dysfunction in a mouse model of type 1 diabetes. Sci Rep. 2019;9:3799 pubmed publisher
  6. Kurundkar D, Kurundkar A, Bone N, Becker E, Liu W, Chacko B, et al. SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury. JCI Insight. 2019;4: pubmed publisher
  7. Wei Z, Song J, Wang G, Cui X, Zheng J, Tang Y, et al. Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis. Nat Commun. 2018;9:4468 pubmed publisher
  8. Peterson B, Campbell J, Ilkayeva O, Grimsrud P, Hirschey M, Newgard C. Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or ? Cell Metabolism in the Absence of Overnutrition. Cell Rep. 2018;24:209-223.e6 pubmed publisher
  9. Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
  10. Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
  11. Hou Y, Lautrup S, Cordonnier S, Wang Y, Croteau D, Zavala E, et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency. Proc Natl Acad Sci U S A. 2018;115:E1876-E1885 pubmed publisher
  12. He X, Zeng H, Chen S, Roman R, Aschner J, Didion S, et al. Endothelial specific SIRT3 deletion impairs glycolysis and angiogenesis and causes diastolic dysfunction. J Mol Cell Cardiol. 2017;112:104-113 pubmed publisher
  13. Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  14. Patil M, Sharma B, Elattar S, Chang J, Kapil S, Yuan J, et al. Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning. Diabetes. 2017;66:1611-1625 pubmed publisher
  15. Gudiksen A, Bertholdt L, Vingborg M, Hansen H, Ringholm S, Pilegaard H. Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2017;312:E204-E214 pubmed publisher
  16. Li J, Chen T, Xiao M, Li N, Wang S, Su H, et al. Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1. Oncotarget. 2016;7:86648-86659 pubmed publisher
  17. Liu Y, Wang T, Zhang R, Fu W, Wang X, Wang F, et al. Calorie restriction protects against experimental abdominal aortic aneurysms in mice. J Exp Med. 2016;213:2473-2488 pubmed
  18. Wawrzyniak N, Joseph A, Levin D, Gundermann D, Leeuwenburgh C, Sandesara B, et al. Idiopathic chronic fatigue in older adults is linked to impaired mitochondrial content and biogenesis signaling in skeletal muscle. Oncotarget. 2016;7:52695-52709 pubmed publisher
  19. Li Y, Choi D, Lee E, Seo S, Lee S, Cho E. Sirtuin 3 (SIRT3) Regulates α-Smooth Muscle Actin (α-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells. J Biol Chem. 2016;291:10277-92 pubmed publisher
  20. Gurt I, Artsi H, Cohen Kfir E, Hamdani G, Ben Shalom G, Feinstein B, et al. The Sirt1 Activators SRT2183 and SRT3025 Inhibit RANKL-Induced Osteoclastogenesis in Bone Marrow-Derived Macrophages and Down-Regulate Sirt3 in Sirt1 Null Cells. PLoS ONE. 2015;10:e0134391 pubmed publisher
  21. Liu R, Fan M, Candas D, Qin L, Zhang X, Eldridge A, et al. CDK1-Mediated SIRT3 Activation Enhances Mitochondrial Function and Tumor Radioresistance. Mol Cancer Ther. 2015;14:2090-102 pubmed publisher
  22. McGlynn L, McCluney S, Jamieson N, Thomson J, MacDonald A, Oien K, et al. SIRT3 & SIRT7: Potential Novel Biomarkers for Determining Outcome in Pancreatic Cancer Patients. PLoS ONE. 2015;10:e0131344 pubmed publisher
  23. Li Y, Wang W, Xu X, Sun S, Xu X, Qu X. {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3. Biomed Res Int. 2015;2015:491205 pubmed publisher
  24. Brandauer J, Andersen M, Kellezi H, Risis S, Frøsig C, Vienberg S, et al. AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD. Front Physiol. 2015;6:85 pubmed publisher
  25. Zeng H, Vaka V, He X, Booz G, Chen J. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss. J Cell Mol Med. 2015;19:1847-56 pubmed publisher
  26. Polletta L, Vernucci E, Carnevale I, Arcangeli T, Rotili D, Palmerio S, et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy. 2015;11:253-70 pubmed publisher
  27. Kim T, Kim H, Kang Y, Yoon S, Lee J, Choi W, et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta. 2015;1850:401-10 pubmed publisher
  28. Le T, Urasaki Y, Pizzorno G. Uridine prevents fenofibrate-induced fatty liver. PLoS ONE. 2014;9:e87179 pubmed publisher
  29. Kuo H, Deluca T, Miller W, Mrksich M. Profiling deacetylase activities in cell lysates with peptide arrays and SAMDI mass spectrometry. Anal Chem. 2013;85:10635-10642 pubmed publisher
  30. Zhang B, Cui S, Bai X, Zhuo L, Sun X, Hong Q, et al. SIRT3 overexpression antagonizes high glucose accelerated cellular senescence in human diploid fibroblasts via the SIRT3-FOXO1 signaling pathway. Age (Dordr). 2013;35:2237-53 pubmed publisher