This is a Validated Antibody Database (VAD) review about human SIRPA, based on 21 published articles (read how Labome selects the articles), using SIRPA antibody in all methods. It is aimed to help Labome visitors find the most suited SIRPA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIRPA synonym: BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP; tyrosine-protein phosphatase non-receptor type substrate 1; CD172 antigen-like family member A; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; inhibitory receptor SHPS-1; macrophage fusion receptor; myd-1 antigen; tyrosine phosphatase SHP substrate 1

BioLegend
mouse monoclonal (SE5A5)
  • flow cytometry; rat; loading ...; fig 1a
BioLegend SIRPA antibody (Biolegend, 323810) was used in flow cytometry on rat samples (fig 1a). Stem Cell Reports (2018) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig 1e
BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig 1e). J Exp Med (2018) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig s2a
BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig s2a). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig 2f
In order to map the lineage of human dendritic cells, BioLegend SIRPA antibody (BioLegend, SE5a5) was used in flow cytometry on human samples (fig 2f). Science (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig s1b
In order to determine the localization of FcgammaRI, FcgammaRII, and SIRPalpha in macrophages, BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig s1b). J Cell Biol (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:1000; loading ...; fig s1c
In order to describe a protocol to generate sinoatrial node-like pacemaker cells, BioLegend SIRPA antibody (BioLegend, 323807) was used in flow cytometry on human samples at 1:1000 (fig s1c). Nat Biotechnol (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:500; fig 1c
In order to study the role of the RAS/MAPK pathway in hypertrophic cardiomyopathy, BioLegend SIRPA antibody (BioLegend, 323807) was used in flow cytometry on human samples at 1:500 (fig 1c). Stem Cell Reports (2016) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BioLegend SIRPA antibody (BioLegen d, 323806) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:500; fig 2g
In order to optimize conditions to promote the proliferation of multipotent cardiovascular progenitor cells, BioLegend SIRPA antibody (Biolegend, 323801) was used in flow cytometry on human samples at 1:500 (fig 2g). Nat Biotechnol (2015) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig 3
In order to study human cord blood and bone marrow for restricted dendritic cell and monocyte progenitors, BioLegend SIRPA antibody (Biolegend, SE5A5) was used in flow cytometry on human samples (fig 3). J Exp Med (2015) ncbi
Bio-Rad
mouse monoclonal (4C7)
  • immunocytochemistry; human; loading ...; fig 2a
In order to determine the localization of FcgammaRI, FcgammaRII, and SIRPalpha in macrophages, Bio-Rad SIRPA antibody (AbD Serotec, 4C7) was used in immunocytochemistry on human samples (fig 2a). J Cell Biol (2017) ncbi
Miltenyi Biotec
human monoclonal (REA144)
  • flow cytometry; human; 1:50; fig 2g
In order to optimize conditions to promote the proliferation of multipotent cardiovascular progenitor cells, Miltenyi Biotec SIRPA antibody (Miltenyi Biotec, 130-099-793) was used in flow cytometry on human samples at 1:50 (fig 2g). Nat Biotechnol (2015) ncbi
human monoclonal (REA144)
  • flow cytometry; human; 1:20
In order to develop protocol for cardiomyocytes differentiation from human pluripotent stem cells, Miltenyi Biotec SIRPA antibody (Miltenyi Biotec, 130-099-785) was used in flow cytometry on human samples at 1:20. Methods Mol Biol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-8)
  • western blot; human; 1:50; fig 4
Santa Cruz Biotechnology SIRPA antibody (Santa Cruz, sc-373896) was used in western blot on human samples at 1:50 (fig 4). BMC Immunol (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; fig 3b
  • western blot; human; fig 4d
In order to identify genes that contribute to human papilloma virus-16 associated oropharyngeal cancer, Invitrogen SIRPA antibody (Thermo Scientific, PA5-29544) was used in immunohistochemistry - paraffin section on human samples (fig 3b) and in western blot on human samples (fig 4d). Sci Rep (2017) ncbi
mouse monoclonal (15-414)
  • flow cytometry; human
Invitrogen SIRPA antibody (eBioscience, 15-414) was used in flow cytometry on human samples . J Immunol (2014) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; human; loading ...; fig 1b
In order to discover that SIRPalpha is highly expressed in human renal cell carcinoma and melanoma, Abcam SIRPA antibody (Abcam, ab53721) was used in immunohistochemistry - frozen section on human samples (fig 1b). JCI Insight (2017) ncbi
SouthernBiotech
mouse monoclonal (74-22-15)
  • flow cytometry; pig; loading ...; fig 3a
SouthernBiotech SIRPA antibody (SouthernBiotech, 4525-09) was used in flow cytometry on pig samples (fig 3a). Antiviral Res (2018) ncbi
mouse monoclonal (74-22-15)
  • flow cytometry; pig; 1:400; loading ...; fig 4b
In order to use CRISPR/Cas9 to generate pigs with a deletion of Exon 7 of the CD163 gene, encoding the scavenger receptor cysteine-rich domain 5, SouthernBiotech SIRPA antibody (SoutherBiotech, 4525-09) was used in flow cytometry on pig samples at 1:400 (fig 4b). PLoS Pathog (2017) ncbi
mouse monoclonal (74-22-15)
  • flow cytometry; pig; 1:400; loading ...; fig 2b, 4b
SouthernBiotech SIRPA antibody (Southern Biotech, 4525-09) was used in flow cytometry on pig samples at 1:400 (fig 2b, 4b). Am J Physiol Gastrointest Liver Physiol (2016) ncbi
EMD Millipore
mouse monoclonal (OX-41)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 2
EMD Millipore SIRPA antibody (Millipore, MAB1407P) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2). Sci Rep (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
EMD Millipore SIRPA antibody (Millipore, 06-729) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2014) ncbi
ProSci
rabbit polyclonal
  • western blot; mouse; loading ...; fig 2b
ProSci SIRPA antibody (ProSci, 1125) was used in western blot on mouse samples (fig 2b). Sci Rep (2017) ncbi
Articles Reviewed
  1. Yang X, Zhou J, He J, Liu J, Wang H, Liu Y, et al. An Immune System-Modified Rat Model for Human Stem Cell Transplantation Research. Stem Cell Reports. 2018;11:514-521 pubmed publisher
  2. Yang H, Zhang J, Zhang X, Shi J, Pan Y, Zhou R, et al. CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Antiviral Res. 2018;151:63-70 pubmed publisher
  3. Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  4. Ring N, Herndler Brandstetter D, Weiskopf K, Shan L, Volkmer J, George B, et al. Anti-SIRP? antibody immunotherapy enhances neutrophil and macrophage antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E10578-E10585 pubmed publisher
  5. See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  6. Shirakabe K, Omura T, Shibagaki Y, Mihara E, Homma K, Kato Y, et al. Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation. Sci Rep. 2017;7:46174 pubmed publisher
  7. Kannan A, Hertweck K, Philley J, Wells R, Dasgupta S. Genetic Mutation and Exosome Signature of Human Papilloma Virus Associated Oropharyngeal Cancer. Sci Rep. 2017;7:46102 pubmed publisher
  8. Lopes F, Bálint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher
  9. Burkard C, Lillico S, Reid E, Jackson B, Mileham A, Ait Ali T, et al. Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function. PLoS Pathog. 2017;13:e1006206 pubmed publisher
  10. Yanagita T, Murata Y, Tanaka D, Motegi S, Arai E, Daniwijaya E, et al. Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy. JCI Insight. 2017;2:e89140 pubmed publisher
  11. Protze S, Liu J, Nussinovitch U, Ohana L, Backx P, Gepstein L, et al. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker. Nat Biotechnol. 2017;35:56-68 pubmed publisher
  12. Bener G, J Félix A, Sánchez de Diego C, Pascual Fabregat I, Ciudad C, Noe V. Silencing of CD47 and SIRP? by Polypurine reverse Hoogsteen hairpins to promote MCF-7 breast cancer cells death by PMA-differentiated THP-1 cells. BMC Immunol. 2016;17:32 pubmed
  13. Josowitz R, Mulero Navarro S, Rodriguez N, Falce C, Cohen N, Ullian E, et al. Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes. Stem Cell Reports. 2016;7:355-369 pubmed publisher
  14. Sauter K, Waddell L, Lisowski Z, Young R, Lefèvre L, Davis G, et al. Macrophage colony-stimulating factor (CSF1) controls monocyte production and maturation and the steady-state size of the liver in pigs. Am J Physiol Gastrointest Liver Physiol. 2016;311:G533-47 pubmed publisher
  15. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  16. Birket M, Ribeiro M, Verkerk A, Ward D, Leitoguinho A, Den Hartogh S, et al. Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells. Nat Biotechnol. 2015;33:970-9 pubmed publisher
  17. Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
  18. Nagai J, Kitamura Y, Owada K, Yamashita N, Takei K, Goshima Y, et al. Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation. Sci Rep. 2015;5:8269 pubmed publisher
  19. van den Berg C, Elliott D, Braam S, Mummery C, Davis R. Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes Under Defined Conditions. Methods Mol Biol. 2016;1353:163-80 pubmed publisher
  20. Huichalaf C, Micheloni S, Ferri G, Caccia R, Gabellini D. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS ONE. 2014;9:e115278 pubmed publisher
  21. Balan S, Ollion V, Colletti N, Chelbi R, Montanana Sanchis F, Liu H, et al. Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J Immunol. 2014;193:1622-35 pubmed publisher