This is a Validated Antibody Database (VAD) review about human SIGMAR1, based on 11 published articles (read how Labome selects the articles), using SIGMAR1 antibody in all methods. It is aimed to help Labome visitors find the most suited SIGMAR1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIGMAR1 synonym: ALS16; DSMA2; OPRS1; SIG-1R; SR-BP; SR-BP1; SRBP; hSigmaR1; sigma1R

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (F-5)
  • western blot; human; 1:1000; fig 1
  • western blot; rat; 1:1000; fig 1
  • immunohistochemistry knockout validation; mouse; fig 2
In order to utilize a highly specific antibody to monitor sigma-1 receptor expression in the dorsal root ganglion, Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz, SC-166392) was used in western blot on human samples at 1:1000 (fig 1), in western blot on rat samples at 1:1000 (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 2). Neuroscience (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-5)
  • immunohistochemistry knockout validation; mouse; fig 2
In order to utilize a highly specific antibody to monitor sigma-1 receptor expression in the dorsal root ganglion, Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz, SC-137075) was used in immunohistochemistry knockout validation on mouse samples (fig 2). Neuroscience (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 4b
  • western blot knockout validation; mouse; 1:250; loading ...; fig 4c
Abcam SIGMAR1 antibody (Abcam, 223702) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 4b) and in western blot knockout validation on mouse samples at 1:250 (fig 4c). Int J Mol Sci (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-5)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s5a
Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz, sc-137075) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig s5a). Cell (2019) ncbi
mouse monoclonal (B-5)
  • immunocytochemistry; human; 1:100; loading ...; fig 5c
Santa Cruz Biotechnology SIGMAR1 antibody (Santa, sc-137075) was used in immunocytochemistry on human samples at 1:100 (fig 5c). J Cell Biol (2019) ncbi
mouse monoclonal (F-5)
  • western blot; human; 1:1000; fig 1
  • western blot; rat; 1:1000; fig 1
  • immunohistochemistry knockout validation; mouse; fig 2
In order to utilize a highly specific antibody to monitor sigma-1 receptor expression in the dorsal root ganglion, Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz, SC-166392) was used in western blot on human samples at 1:1000 (fig 1), in western blot on rat samples at 1:1000 (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 2). Neuroscience (2016) ncbi
mouse monoclonal (B-5)
  • immunohistochemistry knockout validation; mouse; fig 2
In order to utilize a highly specific antibody to monitor sigma-1 receptor expression in the dorsal root ganglion, Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz, SC-137075) was used in immunohistochemistry knockout validation on mouse samples (fig 2). Neuroscience (2016) ncbi
mouse monoclonal (F-5)
  • western blot; human; 1:1000; fig 4a
Santa Cruz Biotechnology SIGMAR1 antibody (santa cruz, sc-166392) was used in western blot on human samples at 1:1000 (fig 4a). PLoS ONE (2016) ncbi
mouse monoclonal (B-5)
  • western blot; mouse; 1:1000
Santa Cruz Biotechnology SIGMAR1 antibody (Santa Cruz Biotechnology, sc-137075) was used in western blot on mouse samples at 1:1000. J Pharmacol Exp Ther (2014) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 4b
  • western blot knockout validation; mouse; 1:250; loading ...; fig 4c
Abcam SIGMAR1 antibody (Abcam, 223702) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 4b) and in western blot knockout validation on mouse samples at 1:250 (fig 4c). Int J Mol Sci (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 1a
  • immunohistochemistry; mouse; loading ...; fig 3a
  • western blot; mouse; 1:1000; loading ...; fig 1a
Abcam SIGMAR1 antibody (Abcam, ab53852) was used in immunocytochemistry on mouse samples (fig 1a), in immunohistochemistry on mouse samples (fig 3a) and in western blot on mouse samples at 1:1000 (fig 1a). Transl Psychiatry (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3i
Abcam SIGMAR1 antibody (Abcam, ab53852) was used in western blot on mouse samples (fig 3i). Cell (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 2e
  • immunoprecipitation; mouse; fig 5b
  • western blot; mouse; 1:1000; fig 2a
In order to assess the contributions of Sig-1Rs and connexin 43 to the induction of central neuropathic pain during spinal cord injury, Abcam SIGMAR1 antibody (Abcam, ab53852) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 2e), in immunoprecipitation on mouse samples (fig 5b) and in western blot on mouse samples at 1:1000 (fig 2a). Neuropharmacology (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4J2E)
  • western blot; mouse; loading ...; fig s3a
Cell Signaling Technology SIGMAR1 antibody (Cell Signaling, 61994) was used in western blot on mouse samples (fig s3a). Sci Adv (2019) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4a
MilliporeSigma SIGMAR1 antibody (Sigma-Aldrich, HPA018002) was used in western blot on mouse samples (fig 4a). Cell Rep (2018) ncbi
Articles Reviewed
  1. Gaja Capdevila N, Hernández N, Zamanillo D, Vela J, Merlos M, Navarro X, et al. Neuroprotective Effects of Sigma 1 Receptor Ligands on Motoneuron Death after Spinal Root Injury in Mice. Int J Mol Sci. 2021;22: pubmed publisher
  2. Morales Garcia J, Calleja Conde J, Lopez Moreno J, Alonso Gil S, Sanz Sancristobal M, Riba J, et al. N,N-dimethyltryptamine compound found in the hallucinogenic tea ayahuasca, regulates adult neurogenesis in vitro and in vivo. Transl Psychiatry. 2020;10:331 pubmed publisher
  3. Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
  4. Hernández Alvarez M, Sebastian D, Vives S, Ivanova S, Bartoccioni P, Kakimoto P, et al. Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease. Cell. 2019;177:881-895.e17 pubmed publisher
  5. Wheeler M, Jaronen M, Covacu R, Zandee S, Scalisi G, Rothhammer V, et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation. Cell. 2019;176:581-596.e18 pubmed publisher
  6. Niu F, Liao K, Hu G, Sil S, Callen S, Guo M, et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J Cell Biol. 2019;218:700-721 pubmed publisher
  7. D Eletto M, Rossin F, Occhigrossi L, Farrace M, Faccenda D, Desai R, et al. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Rep. 2018;25:3573-3581.e4 pubmed publisher
  8. Choi S, Roh D, Yoon S, Kwon S, Choi H, Han H, et al. Astrocyte sigma-1 receptors modulate connexin 43 expression leading to the induction of below-level mechanical allodynia in spinal cord injured mice. Neuropharmacology. 2016;111:34-46 pubmed publisher
  9. Mavlyutov T, Duellman T, Kim H, Epstein M, Leese C, Davletov B, et al. Sigma-1 receptor expression in the dorsal root ganglion: Reexamination using a highly specific antibody. Neuroscience. 2016;331:148-57 pubmed publisher
  10. Martínez Pizarro A, Desviat L, Ugarte M, Perez B, Richard E. Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects. PLoS ONE. 2016;11:e0150357 pubmed publisher
  11. Sánchez Fernández C, Montilla García Á, González Cano R, Nieto F, Romero L, Artacho Cordón A, et al. Modulation of peripheral ?-opioid analgesia by ?1 receptors. J Pharmacol Exp Ther. 2014;348:32-45 pubmed publisher