This is a Validated Antibody Database (VAD) review about human SIGLEC7, based on 8 published articles (read how Labome selects the articles), using SIGLEC7 antibody in all methods. It is aimed to help Labome visitors find the most suited SIGLEC7 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIGLEC7 synonym: AIRM1; CD328; CDw328; D-siglec; QA79; SIGLEC-7; SIGLEC19P; SIGLECP2; p75; p75/AIRM1

BioLegend
mouse monoclonal (6-434)
  • flow cytometry; human; 2 ug/ml; loading ...; fig 1b
BioLegend SIGLEC7 antibody (Biolegend, 6-434) was used in flow cytometry on human samples at 2 ug/ml (fig 1b). J Clin Invest (2019) ncbi
mouse monoclonal (6-434)
  • flow cytometry; human; loading ...; fig 5a
In order to detail MAIT cell responses to various microorganisms and cytokines, BioLegend SIGLEC7 antibody (BioLegend, 6-434) was used in flow cytometry on human samples (fig 5a). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (6-434)
  • flow cytometry; human; loading ...; fig 4a
In order to assess the effects of sialic acid blockade on dendritic cells, BioLegend SIGLEC7 antibody (Biolegend, 6-434) was used in flow cytometry on human samples (fig 4a). Immunol Cell Biol (2017) ncbi
mouse monoclonal (6-434)
  • flow cytometry; human; 1:100; fig 1j
In order to investigate the impact of a cancer-specific MUC1 glycoform, BioLegend SIGLEC7 antibody (Biolegend, 6-434) was used in flow cytometry on human samples at 1:100 (fig 1j). Nat Immunol (2016) ncbi
mouse monoclonal (S7.7)
  • blocking or activating experiments; human
BioLegend SIGLEC7 antibody (BioLegend, S7.7) was used in blocking or activating experiments on human samples . J Immunol (2014) ncbi
Miltenyi Biotec
human monoclonal (REA214)
  • flow cytometry; human; fig 2a
  • immunocytochemistry; human; fig 2g
  • ELISA; human; fig 2b
Miltenyi Biotec SIGLEC7 antibody (Miltenyi, 130-100-979) was used in flow cytometry on human samples (fig 2a), in immunocytochemistry on human samples (fig 2g) and in ELISA on human samples (fig 2b). Nat Commun (2021) ncbi
human monoclonal (REA214)
  • flow cytometry; human; loading ...; tbl 1
Miltenyi Biotec SIGLEC7 antibody (Miltenyi Biotech, REA214) was used in flow cytometry on human samples (tbl 1). Sci Rep (2016) ncbi
Invitrogen
mouse monoclonal (eBioQA79 (QA79))
  • flow cytometry; human
Invitrogen SIGLEC7 antibody (eBioscience, QA79) was used in flow cytometry on human samples . J Immunol (2014) ncbi
BD Biosciences
mouse monoclonal (F023-420)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences SIGLEC7 antibody (BD, 558372) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Articles Reviewed
  1. Rodriguez E, Boelaars K, Brown K, Eveline Li R, Kruijssen L, Bruijns S, et al. Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9. Nat Commun. 2021;12:1270 pubmed publisher
  2. Duan S, Koziol White C, Jester W, Nycholat C, Macauley M, Panettieri R, et al. CD33 recruitment inhibits IgE-mediated anaphylaxis and desensitizes mast cells to allergen. J Clin Invest. 2019;129:1387-1401 pubmed publisher
  3. Dias J, Leeansyah E, Sandberg J. Multiple layers of heterogeneity and subset diversity in human MAIT cell responses to distinct microorganisms and to innate cytokines. Proc Natl Acad Sci U S A. 2017;114:E5434-E5443 pubmed publisher
  4. Bull C, Collado Camps E, Kers Rebel E, Heise T, Søndergaard J, den Brok M, et al. Metabolic sialic acid blockade lowers the activation threshold of moDCs for TLR stimulation. Immunol Cell Biol. 2017;95:408-415 pubmed publisher
  5. Madge P, Maggioni A, Pascolutti M, Amin M, Waespy M, Bellette B, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy. Sci Rep. 2016;6:36012 pubmed publisher
  6. Beatson R, Tajadura Ortega V, Achkova D, Picco G, Tsourouktsoglou T, Klausing S, et al. The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9. Nat Immunol. 2016;17:1273-1281 pubmed publisher
  7. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  8. Kawasaki N, Rillahan C, Cheng T, Van Rhijn I, Macauley M, Moody D, et al. Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation. J Immunol. 2014;193:1560-6 pubmed publisher