This is a Validated Antibody Database (VAD) review about human SIGLEC5, based on 6 published articles (read how Labome selects the articles), using SIGLEC5 antibody in all methods. It is aimed to help Labome visitors find the most suited SIGLEC5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIGLEC5 synonym: CD170; CD33L2; OB-BP2; OBBP2; SIGLEC-5; sialic acid-binding Ig-like lectin 5; CD33 antigen-like 2; OB-binding protein 2; obesity-binding protein 2; sialic acid-binding immunoglobulin-like lectin 5

R&D Systems
mouse monoclonal (194128)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1e
R&D Systems SIGLEC5 antibody (R&D Systems, MAB10721) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1e). Am J Respir Crit Care Med (2018) ncbi
goat polyclonal
  • immunoprecipitation; human; loading ...; fig 3b
R&D Systems SIGLEC5 antibody (R&D Systems, AF5806) was used in immunoprecipitation on human samples (fig 3b). EMBO J (2017) ncbi
mouse monoclonal (194128)
  • immunocytochemistry; human; loading ...; fig 3b
In order to show that PSGL1 is a ligand for Siglec-5, R&D Systems SIGLEC5 antibody (R&D Systems, MAB10721) was used in immunocytochemistry on human samples (fig 3b). Sci Rep (2016) ncbi
mouse monoclonal (194128)
  • flow cytometry; human; loading ...; tbl 1
R&D Systems SIGLEC5 antibody (R&D Systems, 194128) was used in flow cytometry on human samples (tbl 1). Sci Rep (2016) ncbi
Miltenyi Biotec
mouse monoclonal (1A5)
  • flow cytometry; human; tbl 1
In order to compare methods of isolating skin mononuclear phagocytes, Miltenyi Biotec SIGLEC5 antibody (Miltenyi Biotech, 1A5) was used in flow cytometry on human samples (tbl 1). J Leukoc Biol (2017) ncbi
mouse monoclonal (1A5)
  • flow cytometry; human; loading ...; tbl 1
Miltenyi Biotec SIGLEC5 antibody (Miltenyi Biotech, 1A5) was used in flow cytometry on human samples (tbl 1). Sci Rep (2016) ncbi
BioLegend
mouse monoclonal (1A5)
  • flow cytometry; human; loading ...; fig 4a
In order to assess the effects of sialic acid blockade on dendritic cells, BioLegend SIGLEC5 antibody (Biolegend, 1A5) was used in flow cytometry on human samples (fig 4a). Immunol Cell Biol (2017) ncbi
Articles Reviewed
  1. Förster K, Sass S, Ehrhardt H, Mous D, Rottier R, Oak P, et al. Early Identification of Bronchopulmonary Dysplasia Using Novel Biomarkers by Proteomic Screening. Am J Respir Crit Care Med. 2018;197:1076-1080 pubmed publisher
  2. Botting R, Bertram K, Baharlou H, Sandgren K, Fletcher J, Rhodes J, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes. J Leukoc Biol. 2017;101:1393-1403 pubmed publisher
  3. Schwarz F, Landig C, Siddiqui S, Secundino I, Olson J, Varki N, et al. Paired Siglec receptors generate opposite inflammatory responses to a human-specific pathogen. EMBO J. 2017;36:751-760 pubmed publisher
  4. Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
  5. Bull C, Collado Camps E, Kers Rebel E, Heise T, Søndergaard J, den Brok M, et al. Metabolic sialic acid blockade lowers the activation threshold of moDCs for TLR stimulation. Immunol Cell Biol. 2017;95:408-415 pubmed publisher
  6. Madge P, Maggioni A, Pascolutti M, Amin M, Waespy M, Bellette B, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy. Sci Rep. 2016;6:36012 pubmed publisher