This is a Validated Antibody Database (VAD) review about human SIAH2, based on 5 published articles (read how Labome selects the articles), using SIAH2 antibody in all methods. It is aimed to help Labome visitors find the most suited SIAH2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIAH2 synonym: hSiah2; E3 ubiquitin-protein ligase SIAH2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia homolog 2; siah-2

Novus Biologicals
mouse monoclonal (24E6H3)
  • western blot; human; 1:500; fig 5
Novus Biologicals SIAH2 antibody (Novus Biologicals, NB110-88113) was used in western blot on human samples at 1:500 (fig 5). Nat Commun (2016) ncbi
mouse monoclonal (24E6H3)
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human; 1:40; fig 4
Novus Biologicals SIAH2 antibody (Novus biologicals, NB110-88113) was used in immunohistochemistry - paraffin section on human samples and in immunohistochemistry on human samples at 1:40 (fig 4). Nat Cell Biol (2015) ncbi
mouse monoclonal (24E6H3)
  • immunohistochemistry - paraffin section; human
In order to screen a panel of 33 proteins to establish a set of biomarkers that predict the agressiveness of prostate cancer, Novus Biologicals SIAH2 antibody (Novus, NB110-88113) was used in immunohistochemistry - paraffin section on human samples . BMC Cancer (2014) ncbi
Sigma-Aldrich
mouse monoclonal (Siah2-369)
  • western blot; human; fig 1
Sigma-Aldrich SIAH2 antibody (Sigma, S7945) was used in western blot on human samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (Siah2-369)
  • immunocytochemistry; human; 1:100-150
  • western blot; human; 1:300-500
In order to elucidate the role of Nrf2 at the blood brain barrier, Sigma-Aldrich SIAH2 antibody (Sigma, S7945) was used in immunocytochemistry on human samples at 1:100-150 and in western blot on human samples at 1:300-500. PLoS ONE (2015) ncbi
mouse monoclonal (Siah2-369)
  • western blot; human; 1:500; fig 2
Sigma-Aldrich SIAH2 antibody (Sigma, S7945) was used in western blot on human samples at 1:500 (fig 2). Nat Cell Biol (2015) ncbi
Articles Reviewed
  1. Ma B, Cheng H, Gao R, Mu C, Chen L, Wu S, et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-? signalling pathways. Nat Commun. 2016;7:11123 pubmed publisher
  2. Adam M, Matt S, Christian S, Hess Stumpp H, Haegebarth A, Hofmann T, et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle. 2015;14:3734-47 pubmed publisher
  3. Sajja R, Green K, Cucullo L. Altered Nrf2 signaling mediates hypoglycemia-induced blood-brain barrier endothelial dysfunction in vitro. PLoS ONE. 2015;10:e0122358 pubmed publisher
  4. Ma B, Chen Y, Chen L, Cheng H, Mu C, Li J, et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat Cell Biol. 2015;17:95-103 pubmed publisher
  5. Rizzardi A, Rosener N, Koopmeiners J, Isaksson Vogel R, Metzger G, Forster C, et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. BMC Cancer. 2014;14:244 pubmed publisher