This is a Validated Antibody Database (VAD) review about human SF3B1, based on 9 published articles (read how Labome selects the articles), using SF3B1 antibody in all methods. It is aimed to help Labome visitors find the most suited SF3B1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SF3B1 synonym: Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155

Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse
  • western blot; mouse; fig 4
In order to propose that a special mechanism of splicing occurs in the testis and brain whereby H2A.B.3 recruits RNA processing factors from splicing speckles, Abcam SF3B1 antibody (Abcam, ab66774) was used in immunocytochemistry on mouse samples and in western blot on mouse samples (fig 4). PLoS Genet (2017) ncbi
domestic rabbit monoclonal (EPR11987(B))
  • western blot; human; 1:1000; loading ...; fig s1c
Abcam SF3B1 antibody (ABCAM, ab170854) was used in western blot on human samples at 1:1000 (fig s1c). Oncogene (2017) ncbi
domestic rabbit monoclonal (EPR11987(B))
  • western blot; human; fig 2d
In order to learn about vulnerability in Ewing sarcoma by splicing of the EWS-FLI1 fusion transcript discovered thrugh functional genomic screening, Abcam SF3B1 antibody (Abcam, ab170854) was used in western blot on human samples (fig 2d). Cell Rep (2016) ncbi
Invitrogen
domestic rabbit polyclonal
In order to report that PRMT1 regulates alternative RNA splicing by reducing RBM15, Invitrogen SF3B1 antibody (Thermo Scientific, PA5-19679) was used . elife (2015) ncbi
MBL International
monoclonal
  • western blot; human; 1:1000; loading ...; fig 7d
MBL International SF3B1 antibody (MBL, D221-3) was used in western blot on human samples at 1:1000 (fig 7d). Oncogenesis (2020) ncbi
monoclonal
  • western blot; human; loading ...; fig e2d
MBL International SF3B1 antibody (MBL, D221-3) was used in western blot on human samples (fig e2d). Nature (2019) ncbi
monoclonal
  • western blot; human; loading ...; fig 5a
MBL International SF3B1 antibody (Medical & Biological Laboratories Co, D221-3) was used in western blot on human samples (fig 5a). Mol Cell (2019) ncbi
monoclonal
  • western blot; human; 1:250; fig 4j
MBL International SF3B1 antibody (MBL International, D221-3) was used in western blot on human samples at 1:250 (fig 4j). PLoS Pathog (2015) ncbi
monoclonal
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 4
MBL International SF3B1 antibody (MBL International, p221-3) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 4). Cancer Res (2015) ncbi
Articles Reviewed
  1. Ailiken G, Kitamura K, Hoshino T, Satoh M, Tanaka N, Minamoto T, et al. Post-transcriptional regulation of BRG1 by FIRΔexon2 in gastric cancer. Oncogenesis. 2020;9:26 pubmed publisher
  2. Inoue D, Chew G, Liu B, Michel B, Pangallo J, D Avino A, et al. Spliceosomal disruption of the non-canonical BAF complex in cancer. Nature. 2019;574:432-436 pubmed publisher
  3. Lee S, Mayr C. Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation. Mol Cell. 2019;: pubmed publisher
  4. Soboleva T, Parker B, Nekrasov M, Hart Smith G, Tay Y, Tng W, et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet. 2017;13:e1006633 pubmed publisher
  5. Kesarwani A, Ramirez O, Gupta A, Yang X, Murthy T, Minella A, et al. Cancer-associated SF3B1 mutants recognize otherwise inaccessible cryptic 3' splice sites within RNA secondary structures. Oncogene. 2017;36:1123-1133 pubmed publisher
  6. Grohar P, Kim S, Rangel Rivera G, Sen N, Haddock S, Harlow M, et al. Functional Genomic Screening Reveals Splicing of the EWS-FLI1 Fusion Transcript as a Vulnerability in Ewing Sarcoma. Cell Rep. 2016;14:598-610 pubmed publisher
  7. Zhang L, Tran N, Su H, Wang R, Lu Y, Tang H, et al. Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. elife. 2015;4: pubmed publisher
  8. Schreiber C, Sakuma T, Izumiya Y, Holditch S, Hickey R, Bressin R, et al. An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. PLoS Pathog. 2015;11:e1005082 pubmed publisher
  9. Zhou Q, Derti A, Ruddy D, Rakiec D, Kao I, Lira M, et al. A chemical genetics approach for the functional assessment of novel cancer genes. Cancer Res. 2015;75:1949-58 pubmed publisher