This is a Validated Antibody Database (VAD) review about human SETDB1, based on 13 published articles (read how Labome selects the articles), using SETDB1 antibody in all methods. It is aimed to help Labome visitors find the most suited SETDB1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SETDB1 synonym: ESET; H3-K9-HMTase4; KG1T; KMT1E; TDRD21; histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; ERG-associated protein with a SET domain, ESET; H3-K9-HMTase 4; histone H3-K9 methyltransferase 4; histone-lysine N-methyltransferase, H3lysine-9 specific 4; lysine N-methyltransferase 1E; tudor domain containing 21

Knockout validation
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2h
  • chromatin immunoprecipitation; human; loading ...; fig 3f
Proteintech Group SETDB1 antibody (Proteintech, 11231-1-AP) was used in western blot knockout validation on human samples at 1:1000 (fig 2h) and in chromatin immunoprecipitation on human samples (fig 3f). Nature (2018) ncbi
Invitrogen
mouse monoclonal (5H6A12)
  • immunohistochemistry; mouse; loading ...; fig s16d
  • western blot; mouse; loading ...; fig s20a
Invitrogen SETDB1 antibody (Thermo Scientific, MA5-15722) was used in immunohistochemistry on mouse samples (fig s16d) and in western blot on mouse samples (fig s20a). Science (2019) ncbi
mouse monoclonal (5H6A12)
  • western blot; mouse; 1:1000; fig 2
In order to characterize TRIM28 as an epigenetic barrier to generate reprogramming of pluripotent stem cells, Invitrogen SETDB1 antibody (Thermo Fisher, MA5-15722) was used in western blot on mouse samples at 1:1000 (fig 2). Stem Cells (2017) ncbi
mouse monoclonal (5H6D4)
  • immunoprecipitation; human; 1:150; fig 1
  • western blot; human; 1:1000; fig s3
In order to investigate p63 and SETDB1 expression in breast cancer cells, Invitrogen SETDB1 antibody (Thermo Scientific, 5H6D4) was used in immunoprecipitation on human samples at 1:150 (fig 1) and in western blot on human samples at 1:1000 (fig s3). Oncotarget (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; human; fig 1
Abcam SETDB1 antibody (Abcam, ab12317) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:1000; fig 5,6
In order to protect preimplantation mouse embryos from endogenous retrotransposons by histone chaperone CAF-1 mediating repressive histone modifications, Abcam SETDB1 antibody (Abcam, ab12317) was used in immunocytochemistry on mouse samples at 1:1000 (fig 5,6). Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
Abcam SETDB1 antibody (Abcam, ab12317) was used in immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 4). Nucleic Acids Res (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam SETDB1 antibody (Abcam, ab12317) was used in western blot on human samples . Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
Abcam SETDB1 antibody (Abcam, ab12317) was used in chromatin immunoprecipitation on mouse samples and in western blot on mouse samples . J Virol (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam SETDB1 antibody (Abcam, ab12317) was used in western blot on human samples . Oncogene (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam SETDB1 antibody (Abcam, ab12317) was used in western blot on human samples . Nucleic Acids Res (2012) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2h
  • chromatin immunoprecipitation; human; loading ...; fig 3f
Proteintech Group SETDB1 antibody (Proteintech, 11231-1-AP) was used in western blot knockout validation on human samples at 1:1000 (fig 2h) and in chromatin immunoprecipitation on human samples (fig 3f). Nature (2018) ncbi
rabbit polyclonal
  • western blot; human
  • western blot; mouse
Proteintech Group SETDB1 antibody (Protein Group, 11231-1-AP) was used in western blot on human samples and in western blot on mouse samples . J Biol Chem (2013) ncbi
Cell Signaling Technology
rabbit monoclonal (C1C12)
  • immunocytochemistry; mouse
Cell Signaling Technology SETDB1 antibody (Cell Signaling Technology, 2196) was used in immunocytochemistry on mouse samples . Blood (2014) ncbi
Articles Reviewed
  1. Nicetto D, Donahue G, Jain T, Peng T, Sidoli S, Sheng L, et al. H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification. Science. 2019;363:294-297 pubmed publisher
  2. Zhu Y, Wang G, Cingoz O, Goff S. NP220 mediates silencing of unintegrated retroviral DNA. Nature. 2018;564:278-282 pubmed publisher
  3. Miles D, de Vries N, Gisler S, Lieftink C, Akhtar W, Gogola E, et al. TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming. Stem Cells. 2017;35:147-157 pubmed publisher
  4. Alarcón V, Hernández S, Rubio L, Alvarez F, Flores Y, Varas Godoy M, et al. The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state. Sci Rep. 2016;6:25901 pubmed publisher
  5. Regina C, Compagnone M, Peschiaroli A, Lena A, Annicchiarico Petruzzelli M, Piro M, et al. Setdb1, a novel interactor of ΔNp63, is involved in breast tumorigenesis. Oncotarget. 2016;7:28836-48 pubmed publisher
  6. Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama E, et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 2015;112:14641-6 pubmed publisher
  7. Rivera C, Saavedra F, Alvarez F, Díaz Celis C, Ugalde V, Li J, et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res. 2015;43:9097-106 pubmed publisher
  8. Cho S, Park J, Kang Y. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res. 2014;42:13545-56 pubmed publisher
  9. Hattangadi S, Martinez Morilla S, Patterson H, Shi J, Burke K, Avila Figueroa A, et al. Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood. 2014;124:1931-40 pubmed
  10. Kao T, Liao H, Wolf D, Tai K, Chuang C, Lee H, et al. Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells. J Virol. 2014;88:10680-95 pubmed publisher
  11. Rodríguez Paredes M, Martinez de Paz A, Simo Riudalbas L, Sayols S, Moutinho C, Moran S, et al. Gene amplification of the histone methyltransferase SETDB1 contributes to human lung tumorigenesis. Oncogene. 2014;33:2807-13 pubmed publisher
  12. Kera Y, Katoh Y, Ohta M, Matsumoto M, Takano Yamamoto T, Igarashi K. Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus. J Biol Chem. 2013;288:13592-601 pubmed publisher
  13. Mund A, Schubert T, Staege H, Kinkley S, Reumann K, Kriegs M, et al. SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response. Nucleic Acids Res. 2012;40:11363-79 pubmed publisher